| Proline accumulation and metabolism-related genes expression profiles in Kosteletzkya virginica seedlings under salt stress | |
| Wang, HY; Tang, XL; Wang, HL; Shao, HB; Shao, HB (reprint author), Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Biol & Bioresources Utilizat, Yantai 264003, Peoples R China. [email protected] | |
| 发表期刊 | FRONTIERS IN PLANT SCIENCE
 |
| ISSN | 1664-462X |
| 2015-09-29 | |
| 卷号 | 6 |
| 关键词 | Kosteletzlcya Virginica Salt Stress Proline Metabolism Proline Accumulation Expression Profiles |
| DOI | 10.3389/fpls.2015.00792 |
| 产权排序 | [Wang, Hongyan; Tang, Xiaoli; Shao, Hong-Bo] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Biol & Bioresources Utilizat, Yantai 264003, Peoples R China; [Wang, Hongyan; Wang, Honglei] China Agr Univ, Yantai Acad, Yantai, Peoples R China; [Wang, Hongyan; Tang, Xiaoli] Univ Chinese Acad Sci, Beijing, Peoples R China; [Shao, Hong-Bo] Jiangsu Acad Agr Sci, Inst Agrobiotechnol, Nanjing, Jiangsu, Peoples R China |
| 作者部门 | 海岸带生物学与生物资源利用所重点实验室 |
| 英文摘要 | Proline accumulation is a common response to salt stress in many plants. Salt stress also increased proline concentration in roots, stems, and leaves of Kosteletzkya virginica seedling treated with 300 mM NaCl for 24 h and reached 3.75-, 4.76-, and 6.83- fold higher than controls. Further study on proline content in leaves under salt stress showed that proline content increased with increasing NaCl concentrations or time. The proline level peaked at 300 mM NaCl for 24 h and reached more than sixfold higher than control, but at 400 mM NaCl for 24 h proline content fell back slightly along with wilting symptom. To explore the cause behind proline accumulation, we first cloned full length genes related to proline metabolism including KvP5CS1, KvOAT, KvPDH, and KvProT from K. virginica and investigated their expression profiles. The results revealed that the expressions of KvP5CS1 and KvProT were sharply up-regulated by salt stress and the expression of KvOAT showed a slight increase with increasing salt concentrations or time, while the expression of KvPDH was not changed much and slightly decreased before 12 h and then returned to the original level. As the key enzyme genes for proline biosynthesis, the up-regulated expression of KvP5CS1 played a more important role than KvOAT for proline accumulation in leaves under salt stress. The low expression of KvPDH for proline catabolism also made a contribution to proline accumulation before 12 h.; Proline accumulation is a common response to salt stress in many plants. Salt stress also increased proline concentration in roots, stems, and leaves of Kosteletzkya virginica seedling treated with 300 mM NaCl for 24 h and reached 3.75-, 4.76-, and 6.83- fold higher than controls. Further study on proline content in leaves under salt stress showed that proline content increased with increasing NaCl concentrations or time. The proline level peaked at 300 mM NaCl for 24 h and reached more than sixfold higher than control, but at 400 mM NaCl for 24 h proline content fell back slightly along with wilting symptom. To explore the cause behind proline accumulation, we first cloned full length genes related to proline metabolism including KvP5CS1, KvOAT, KvPDH, and KvProT from K. virginica and investigated their expression profiles. The results revealed that the expressions of KvP5CS1 and KvProT were sharply up-regulated by salt stress and the expression of KvOAT showed a slight increase with increasing salt concentrations or time, while the expression of KvPDH was not changed much and slightly decreased before 12 h and then returned to the original level. As the key enzyme genes for proline biosynthesis, the up-regulated expression of KvP5CS1 played a more important role than KvOAT for proline accumulation in leaves under salt stress. The low expression of KvPDH for proline catabolism also made a contribution to proline accumulation before 12 h. |
| 文章类型 | Article |
| 收录类别 | SCI |
| 语种 | 英语 |
| 关键词[WOS] | HIGH-YIELDING GENOTYPES ; MORUS-ALBA L. ; 1ST 2 STEPS ; DELTA(1)-PYRROLINE-5-CARBOXYLATE SYNTHETASE ; ARABIDOPSIS-THALIANA ; TRANSCRIPTIONAL REGULATION ; DEHYDROGENASE GENES ; PLANT DEVELOPMENT ; GLYCINE BETAINE ; OSMOTIC-STRESS |
| 研究领域[WOS] | Plant Sciences |
| WOS记录号 | WOS:000362372400001 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.yic.ac.cn/handle/133337/9754 |
| 专题 | 海岸带生物学与生物资源利用重点实验室_海岸带生物学与生物资源保护实验室 |
| 通讯作者 | Shao, HB (reprint author), Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Biol & Bioresources Utilizat, Yantai 264003, Peoples R China. [email protected] |
| 推荐引用方式 GB/T 7714 | Wang, HY,Tang, XL,Wang, HL,et al. Proline accumulation and metabolism-related genes expression profiles in Kosteletzkya virginica seedlings under salt stress[J]. FRONTIERS IN PLANT SCIENCE,2015,6. |
| APA | Wang, HY,Tang, XL,Wang, HL,Shao, HB,&Shao, HB .(2015).Proline accumulation and metabolism-related genes expression profiles in Kosteletzkya virginica seedlings under salt stress.FRONTIERS IN PLANT SCIENCE,6. |
| MLA | Wang, HY,et al."Proline accumulation and metabolism-related genes expression profiles in Kosteletzkya virginica seedlings under salt stress".FRONTIERS IN PLANT SCIENCE 6(2015). |
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