| Separation of vitexin-4 ''-O-glucoside and vitexin-2 ''-O-rhamnoside from hawthorn leaves extracts using macroporous resins |
| Li, HJ; Liu, Y ; Jin, HZ; Liu, SJ; Fang, ST; Wang, CH; Xia, CH; Xia, CH (reprint author), Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China. [email protected]; [email protected]
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| 发表期刊 | JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
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| ISSN | 1570-0232
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| 2015-12-15
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| 卷号 | 1007页码:23-29 |
| 关键词 | Macroporous Resins
Vitexin-4 ''-o-glucoside
Vitexin-2 ''-o-rhamnoside
Purification
Hawthorn Leaves
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| DOI | 10.1016/j.jchromb.2015.10.043
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| 产权排序 | [Li, Hongjuan; Liu, Ying; Liu, Sujing; Fang, Shengtao; Xia, Chuanhai] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Biol & Biol Resources Utilizat, Yantai 264003, Peoples R China; [Li, Hongjuan; Wang, Chunhua] Binzhou Med Univ, Sch Pharm, Yantai 264003, Peoples R China; [Jin, Haizhu] Yantai Univ, Sch Life Sci, Yantai 264005, Peoples R China; [Li, Hongjuan] Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 作者部门 | 海岸带生物学与生物资源利用所重点实验室
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| 英文摘要 | Vitexin-4 ''-O-glucoside and vitexin-2 ''-O-rhamnoside are the major flavonoids of hawthorn leaves. In this work, the adsorption and desorption characteristics of vitexin-4 ''-O-glucoside and vitexin-2 ''-O-rhamnoside on seven macroporous resins were evaluated. Among the tested resins, the HPD-400 resin showed the best adsorption and desorption capacities. Adsorption isotherms were constructed for the HPD-400 resin and well fitted to Langmuir and Freundlich models. Dynamic adsorption and desorption tests were performed on column packed with the HPD-400 resin to optimize the chromatographic parameters. After one run treatment with the HPD-400 resin, the contents of vitexin-4 ''-O-glucoside and vitexin-2 ''-O-rhamnoside in the product were increased 8.44-fold and 8.43-fold from 0.720% and 2.63% to 6.08% and 22.2% with recovery yields of 79.1% and 81.2%, respectively. These results show that the developed method is a promising basis for the large-scale purification of vitexin-4 ''-O-glucoside and vitexin-2 ''-O-rhamnoside from hawthorn leaves and other plant materials. (C) 2015 Elsevier B.V. All rights reserved.; Vitexin-4 ''-O-glucoside and vitexin-2 ''-O-rhamnoside are the major flavonoids of hawthorn leaves. In this work, the adsorption and desorption characteristics of vitexin-4 ''-O-glucoside and vitexin-2 ''-O-rhamnoside on seven macroporous resins were evaluated. Among the tested resins, the HPD-400 resin showed the best adsorption and desorption capacities. Adsorption isotherms were constructed for the HPD-400 resin and well fitted to Langmuir and Freundlich models. Dynamic adsorption and desorption tests were performed on column packed with the HPD-400 resin to optimize the chromatographic parameters. After one run treatment with the HPD-400 resin, the contents of vitexin-4 ''-O-glucoside and vitexin-2 ''-O-rhamnoside in the product were increased 8.44-fold and 8.43-fold from 0.720% and 2.63% to 6.08% and 22.2% with recovery yields of 79.1% and 81.2%, respectively. These results show that the developed method is a promising basis for the large-scale purification of vitexin-4 ''-O-glucoside and vitexin-2 ''-O-rhamnoside from hawthorn leaves and other plant materials. (C) 2015 Elsevier B.V. All rights reserved. |
| 文章类型 | Article
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| 资助机构 | National Key Technology Research and Development Program of Ministry of Science and Technology of China(2011BAC02B04)
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| 收录类别 | SCI
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| 语种 | 英语
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| 关键词[WOS] | UPLC-ESI-MS/MS
; PREPARATIVE SEPARATION
; CHLOROGENIC ACID
; FLAVONOIDS
; PURIFICATION
; ENRICHMENT
; HPLC
; VITEXIN-2-O-RHAMNOSIDE
; ADSORPTION
; VITEXIN
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| 研究领域[WOS] | Biochemistry & Molecular Biology
; Chemistry
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| WOS记录号 | WOS:000366772600004
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| 引用统计 |
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| 文献类型 | 期刊论文
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| 条目标识符 | http://ir.yic.ac.cn/handle/133337/9308
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| 专题 | 海岸带生物学与生物资源利用重点实验室_海岸带生物学与生物资源保护实验室
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| 通讯作者 | Xia, CH (reprint author), Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China. [email protected]; [email protected] |
推荐引用方式
GB/T 7714 |
Li, HJ,Liu, Y,Jin, HZ,et al. Separation of vitexin-4 ''-O-glucoside and vitexin-2 ''-O-rhamnoside from hawthorn leaves extracts using macroporous resins[J]. JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,2015,1007:23-29.
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| APA |
Li, HJ.,Liu, Y.,Jin, HZ.,Liu, SJ.,Fang, ST.,[email protected].(2015).Separation of vitexin-4 ''-O-glucoside and vitexin-2 ''-O-rhamnoside from hawthorn leaves extracts using macroporous resins.JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,1007,23-29.
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| MLA |
Li, HJ,et al."Separation of vitexin-4 ''-O-glucoside and vitexin-2 ''-O-rhamnoside from hawthorn leaves extracts using macroporous resins".JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES 1007(2015):23-29.
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