A near-infrared fluorescent probe for the selective detection of HNO in living cells and in vivo

	A near-infrared fluorescent probe for the selective detection of HNO in living cells and in vivo
	Liu, P; Jing, XT; Yu, FB; Lv, CJ; Chen, LX; Yu, FB (reprint author), Chinese Acad Sci, Yantai Inst Coastal Zone Res, Res Ctr Coastal Environm Engn & Technol, Key Lab Coastal Environm Proc & Ecol Remediat, Yantai 264003, Peoples R China. [email protected]; [email protected]
发表期刊	ANALYST
	2015
卷号	140 期号:13 页码:4576-4583
产权排序	[Liu, Ping; Jing, Xiaotong; Yu, Fabiao; Chen, Lingxin] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Res Ctr Coastal Environm Engn & Technol, Key Lab Coastal Environm Proc & Ecol Remediat, Yantai 264003, Peoples R China; [Yu, Fabiao; Lv, Changjun; Chen, Lingxin] Binzhou Med Univ, Med Res Ctr, Yantai 264003, Peoples R China
作者部门	中科院海岸带环境过程与生态修复重点实验室
英文摘要	Nitroxyl (HNO), the one-electron reduced and protonated analogue of nitric oxide (NO), demonstrates distinctive bio-pharmacological effects in the treatment of cardiovascular disorders. Herein, we design and synthesize a near-infrared (NIR) metal-free fluorescent probe Cyto-JN for the detection of nitroxyl (HNO) in living cells and in vivo. The metal-free Cyto-JN is composed of two moieties: the Aza-BODIPY fluorophore and the HNO recognition unit, the diphenylphosphinobenzoyl group. Cyto-JN can react with HNO in a 1 : 1 stoichiometry, which may bring great benefit to the detection efficiency of bioassays. Cyto-JN shows high sensitivity toward HNO and exhibits low cytotoxic effect on cells. Moreover, the probe displays good selectivity for the detection of HNO in the presence of various biologically related species. Cyto-JN can be applied successfully to bio-imaging of HNO in living cells and in mice. The results of flow cytometry confirm that the probe Cyto-JN can be used to detect intracellular HNO qualitatively and quantitatively.
文章类型	Article
收录类别	SCI
语种	英语
关键词[WOS]	NITRIC-OXIDE ; NITROXYL HNO ; AQUEOUS-SOLUTION ; HYDROGEN POLYSULFIDES ; REDUCTANT-RESISTANT ; ANGELIS SALT ; NO ; PEROXYNITRITE ; QUANTITATION ; CHEMISTRY
研究领域[WOS]	Chemistry
WOS记录号	WOS:000356171400030
引用统计	被引频次：64[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.yic.ac.cn/handle/133337/9258
专题	中国科学院海岸带环境过程与生态修复重点实验室
通讯作者	Yu, FB (reprint author), Chinese Acad Sci, Yantai Inst Coastal Zone Res, Res Ctr Coastal Environm Engn & Technol, Key Lab Coastal Environm Proc & Ecol Remediat, Yantai 264003, Peoples R China. [email protected]; [email protected]
推荐引用方式 GB/T 7714	Liu, P,Jing, XT,Yu, FB,et al. A near-infrared fluorescent probe for the selective detection of HNO in living cells and in vivo[J]. ANALYST,2015,140(13):4576-4583.
APA	Liu, P.,Jing, XT.,Yu, FB.,Lv, CJ.,Chen, LX.,[email protected].(2015).A near-infrared fluorescent probe for the selective detection of HNO in living cells and in vivo.ANALYST,140(13),4576-4583.
MLA	Liu, P,et al."A near-infrared fluorescent probe for the selective detection of HNO in living cells and in vivo".ANALYST 140.13(2015):4576-4583.

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