Simultaneous extraction of four classes of antibiotics in soil, manure and sewage sludge and analysis by liquid chromatography-tandem mass spectrometry with the isotope-labelled internal standard method

	Simultaneous extraction of four classes of antibiotics in soil, manure and sewage sludge and analysis by liquid chromatography-tandem mass spectrometry with the isotope-labelled internal standard method
	Huang, Yujuan 1; Cheng, Miaomiao 1; Li, Wenhong 1; Wu, Longhua 1; Chen, Yongshan 3; Luo, Yongming1,2 ; Christie, Peter 4; Zhang, Haibo2
发表期刊	ANALYTICAL METHODS
	2013
卷号	5 期号:15 页码:3721-3731
英文摘要	An analytical method for the analysis of four classes of antibiotics (tetracyclines, sulfonamides, fluoroquinolones and macrolides) has been developed and validated in this work. After optimizing the extraction and purification, the solvent consisting of a (EDTA-sodium phosphate buffer with acetonitrile : Mg(NO3)(2)-NH3 center dot H2O ,v/v, 3 : 1) was used as the extraction buffer, these antibiotics were extracted from agricultural soil, manure, and sewage sludge using ultrasonic-assisted extraction aided by mechanical shaking and followed by solid phase extraction (SPE) clean-up with hydrophilic-lipophilic balance (HLB) cartridges. The chromatographic separation was optimized, the analytes were separated and detected by liquid chromatography-electrospray ionization tandem mass spectrometry (LC-ESI-MS/MS) and quantified by the isotope-labelled internal standard method. Mass spectral acquisition was done in the positive ion mode by applying multiple reaction monitoring (MRM) of two fragmentation transitions per analyte to provide a high degree of sensitivity and specificity. The calibration range used for all the antibiotics was 5.0-300.0 mu g L-1 and each calibration curve was linear with a coefficient of determination (R-2) > 0.997, the instrument detection limits (IDLs) and quantification limits (IQLs) ranged from 0.09 to 3.57 and 0.49 to 11.9 mu g L-1, respectively. Spiked recoveries were obtained in the range 60-140% for these antibiotics from three different matrices, with the exception of NFC and OFC in soil (52-55%). Lower relative standard deviation (RSD < 17%) for seven replicates from each matrix at 50 mu g kg(-1) spiking level and the method detection limits (MDLs) obtained in the ranges 0.5-14.8 mu g kg(-1) from soil, 0.5-14.1 mu g kg(-1) from manure and 1.3-17.3 mu g kg(-1) from sludge for all the antibiotics studied indicate that the method was reliable and sensitive for extraction and determination of the target compounds in soil, manure and sludge matrices. Finally, the method was applied to analyse soil, manure and sewage sludge samples. Fluoroquinolones were the most dominant antibiotics and there were smaller amounts of sulfonamides in the soil, sludge and manure samples collected.
文章类型	Article
收录类别	SCI
语种	英语
关键词[WOS]	SOLID-PHASE EXTRACTION ; WASTE-WATER TREATMENT ; VETERINARY ANTIBIOTICS ; SULFONAMIDE ANTIBIOTICS ; SURFACE-WATER ; TETRACYCLINE ANTIBIOTICS ; FLUORESCENCE DETECTION ; ENVIRONMENTAL-SAMPLES ; MACROLIDE ANTIBIOTICS ; RESISTANT-BACTERIA
研究领域[WOS]	Chemistry ; Food Science & Technology ; Spectroscopy
WOS记录号	WOS:000321625900018
引用统计	被引频次：91[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.yic.ac.cn/handle/133337/9032
专题	中国科学院海岸带环境过程与生态修复重点实验室
作者单位	1.Chinese Acad Sci, Inst Soil Sci, Key Lab Soil Environm & Pollut Remediat, Nanjing 210008, Jiangsu, Peoples R China 2.Chinese Acad Sci, Yantai Inst Costal Zone Res, Yantai 264003, Peoples R China 3.Chinese Acad Sci, Inst Urban Environm, Xiamen 361021, Peoples R China 4.Agri Food & Biosci Inst, Agri Environm Branch, Belfast BT9 5PX, Antrim, North Ireland
推荐引用方式 GB/T 7714	Huang, Yujuan,Cheng, Miaomiao,Li, Wenhong,et al. Simultaneous extraction of four classes of antibiotics in soil, manure and sewage sludge and analysis by liquid chromatography-tandem mass spectrometry with the isotope-labelled internal standard method[J]. ANALYTICAL METHODS,2013,5(15):3721-3731.
APA	Huang, Yujuan.,Cheng, Miaomiao.,Li, Wenhong.,Wu, Longhua.,Chen, Yongshan.,...&Zhang, Haibo.(2013).Simultaneous extraction of four classes of antibiotics in soil, manure and sewage sludge and analysis by liquid chromatography-tandem mass spectrometry with the isotope-labelled internal standard method.ANALYTICAL METHODS,5(15),3721-3731.
MLA	Huang, Yujuan,et al."Simultaneous extraction of four classes of antibiotics in soil, manure and sewage sludge and analysis by liquid chromatography-tandem mass spectrometry with the isotope-labelled internal standard method".ANALYTICAL METHODS 5.15(2013):3721-3731.