| Identification of a LPS-induced TNF-alpha factor (LITAF) from mollusk Solen grandis and its expression pattern towards PAMPs stimulation | |
Yang, Dinglong1,2 ; Wei, Xiumei1; Yang, Jianmin1; Yang, Jialong3; Xu, Jie1,2; Fang, Jinghui1; Wang, Sheng1,2; Liu, Xiangquan1; Wei, XM (reprint author), Shandong Marine Fisheries Res Inst, Shandong Prov Key Lab Restorat Marine Ecol, Yantai 264006, Peoples R China. [email protected]; [email protected]
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| 发表期刊 | FISH & SHELLFISH IMMUNOLOGY
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| ISSN | 1050-4648 |
| 2013-10-01 | |
| 卷号 | 35期号:4页码:1325-1328 |
| 关键词 | Solen Grandis Litaf Innate Immunity Transcription Factor Real-time Pcr |
| 产权排序 | [Yang, Dinglong; Wei, Xiumei; Yang, Jianmin; Xu, Jie; Fang, Jinghui; Wang, Sheng; Liu, Xiangquan] Shandong Marine Fisheries Res Inst, Shandong Prov Key Lab Restorat Marine Ecol, Yantai 264006, Peoples R China; [Yang, Dinglong; Xu, Jie; Wang, Sheng] Shanghai Ocean Univ, Sch Fisheries & Life Sci, Shanghai 201306, Peoples R China; [Yang, Jialong] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China |
| 英文摘要 | Lipopolysaccharide-induced TNF-alpha factor (LITAF) is one of the most important transcription factors mediating TNF-alpha transcription. In the present study, a LITAF gene (designated as SgLITAF) was identified from razor clams Solen grandis. The full-length cDNA of SgLITAF was of 1476 bp, encoding a polypeptide of 130 amino acids showed high similarity to other known LITAFs. SgLITAF encoded a LITAF domain and the Zn2+-binding motifs in the domain were well conserved. The mRNA transcripts of SgLITAF were detected in all tested tissues of healthy razor clams, including mantle, gill, gonad, hemocytes, muscle and hepatopancreas, and with the highest expression level in hepatopancreas. The expression level of SgLITAF in hemocytes was significantly up-regulated (P < 0.01) after razor clams were stimulated by LPS or beta-1, 3-glucan, but no obvious fluctuation of SgLITAF mRNA expression was observed after PGN stimulation. All the results indicated that there might be a LITAF-regulated TNF-alpha signaling pathway existing in S. grandis, which involved in the immune response not only against gram-negative bacteria but also towards fungi. (C) 2013 Elsevier Ltd. All rights reserved.; Lipopolysaccharide-induced TNF-alpha factor (LITAF) is one of the most important transcription factors mediating TNF-alpha transcription. In the present study, a LITAF gene (designated as SgLITAF) was identified from razor clams Solen grandis. The full-length cDNA of SgLITAF was of 1476 bp, encoding a polypeptide of 130 amino acids showed high similarity to other known LITAFs. SgLITAF encoded a LITAF domain and the Zn2+-binding motifs in the domain were well conserved. The mRNA transcripts of SgLITAF were detected in all tested tissues of healthy razor clams, including mantle, gill, gonad, hemocytes, muscle and hepatopancreas, and with the highest expression level in hepatopancreas. The expression level of SgLITAF in hemocytes was significantly up-regulated (P < 0.01) after razor clams were stimulated by LPS or beta-1, 3-glucan, but no obvious fluctuation of SgLITAF mRNA expression was observed after PGN stimulation. All the results indicated that there might be a LITAF-regulated TNF-alpha signaling pathway existing in S. grandis, which involved in the immune response not only against gram-negative bacteria but also towards fungi. (C) 2013 Elsevier Ltd. All rights reserved. |
| 文章类型 | Article |
| 资助机构 | NSFC [31202025]; Program of Agriculture Thoroughbred Project, Shandong Province, China; Taishan Scholar Position Funding of Aquatic Animal Nutrition and Feed |
| 收录类别 | SCI |
| 语种 | 英语 |
| 关键词[WOS] | MOLECULAR-CLONING ; HOMOLOG ; GENE |
| 研究领域[WOS] | Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences |
| WOS记录号 | WOS:000324849800031 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.yic.ac.cn/handle/133337/7064 |
| 专题 | 中国科学院烟台海岸带研究所知识产出 海岸带生物学与生物资源利用重点实验室_海岸带生物资源高效利用研究与发展中心 |
| 通讯作者 | Wei, XM (reprint author), Shandong Marine Fisheries Res Inst, Shandong Prov Key Lab Restorat Marine Ecol, Yantai 264006, Peoples R China. [email protected]; [email protected] |
| 作者单位 | 1.Shandong Marine Fisheries Res Inst, Shandong Prov Key Lab Restorat Marine Ecol, Yantai 264006, Peoples R China 2.Shanghai Ocean Univ, Sch Fisheries & Life Sci, Shanghai 201306, Peoples R China 3.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China |
| 推荐引用方式 GB/T 7714 | Yang, Dinglong,Wei, Xiumei,Yang, Jianmin,et al. Identification of a LPS-induced TNF-alpha factor (LITAF) from mollusk Solen grandis and its expression pattern towards PAMPs stimulation[J]. FISH & SHELLFISH IMMUNOLOGY,2013,35(4):1325-1328. |
| APA | Yang, Dinglong.,Wei, Xiumei.,Yang, Jianmin.,Yang, Jialong.,Xu, Jie.,[email protected].(2013).Identification of a LPS-induced TNF-alpha factor (LITAF) from mollusk Solen grandis and its expression pattern towards PAMPs stimulation.FISH & SHELLFISH IMMUNOLOGY,35(4),1325-1328. |
| MLA | Yang, Dinglong,et al."Identification of a LPS-induced TNF-alpha factor (LITAF) from mollusk Solen grandis and its expression pattern towards PAMPs stimulation".FISH & SHELLFISH IMMUNOLOGY 35.4(2013):1325-1328. |
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