Institutional Repository of Key Laboratory of Coastal Zone Environmental Processes, Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences (KLCEP)
| Degradation of furazolidone by bacteria Acinetobacter calcoaceticus T32, Pseudomonas putida SP1 and Proteus mirabilis V7 | |
Zhang, Weiwei1,2; Niu, Zongliang1,2; Yin, Kun1,2,3; Liu, Feng4; Chen, Lingxin1,2
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| 发表期刊 | INTERNATIONAL BIODETERIORATION & BIODEGRADATION
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| ISSN | 0964-8305 |
| 2013-02-01 | |
| 卷号 | 77页码:45-50 |
| 关键词 | Furazolidone Bacterial Strain Degradation Glutathione-s-transferase (Gst) Cytotoxicity |
| 产权排序 | [Zhang, Weiwei; Niu, Zongliang; Yin, Kun; Chen, Lingxin] Chinese Acad Sci, Yantai Inst Coastal Zone Res YIC, Key Lab Coastal Zone Environm Proc, Yantai 264003, Peoples R China; [Zhang, Weiwei; Niu, Zongliang; Yin, Kun; Chen, Lingxin] YICCAS, Shandong Prov Key Lab Coastal Zone Environm Proc, Yantai 264003, Shandong, Peoples R China; [Yin, Kun] Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China; [Liu, Feng] China Agr Univ Yantai, Dept Aquaculture, Yantai 264670, Peoples R China |
| 通讯作者 | Chen, LX (reprint author), Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Zone Environm Proc, 17 Chunhui Rd, Yantai 264003, Peoples R China. [email protected] ; [email protected] ; [email protected] ; [email protected] ; [email protected] |
| 作者部门 | 中科院海岸带环境过程与生态修复重点实验室 |
| 英文摘要 | Furazolidone (FZD) has been widely used as an antibacterial and antiprotozoal feed additive for poultry, cattle and farmed fish. Since FZD has been shown to have mutagenic, genotoxic and potentially carcinogenic properties when tested in a variety of systems, there is an increasing need to find a way to remove FZD from contaminated environments. In this report, three bacterial strains Acinetobacter calcoaceticus 132, Pseudomonas putida SP1 and Proteus mirabilis V7 capable of degrading FZD effectively were isolated, identified and characterized. The reduced FZD. concentration after degradation was determined by HPLC. After bacterial cells were grown in the media containing 5 mg l(-1) FZD for 5 days, almost all FZD was degraded by A. calcoaceticus T32, and more than 50% of FZD was degraded by P. putida SP1 and P. mirabilis V7, respectively. Bacterial GST activity of A. calcoaceticus T32, P. putida SP1 and P. mirabilis V7 was determined to be influenced by different FZD concentrations. Cytotoxicity analysis showed that FZD was degraded to the metabolites with far less cytotoxicity compared to FZD. The inoculation of bacterial strains A. calcoaceticus T32, P. putida SP1 and P. mirabilis V7 into FZD-contained media resulted in a higher degradation efficiency than natural degradation, which indicated the potential application of these strains in treatment of FZD-polluted freshwater or seawater environments. (C) 2012 Elsevier Ltd. All rights reserved.; Furazolidone (FZD) has been widely used as an antibacterial and antiprotozoal feed additive for poultry, cattle and farmed fish. Since FZD has been shown to have mutagenic, genotoxic and potentially carcinogenic properties when tested in a variety of systems, there is an increasing need to find a way to remove FZD from contaminated environments. In this report, three bacterial strains Acinetobacter calcoaceticus 132, Pseudomonas putida SP1 and Proteus mirabilis V7 capable of degrading FZD effectively were isolated, identified and characterized. The reduced FZD. concentration after degradation was determined by HPLC. After bacterial cells were grown in the media containing 5 mg l(-1) FZD for 5 days, almost all FZD was degraded by A. calcoaceticus T32, and more than 50% of FZD was degraded by P. putida SP1 and P. mirabilis V7, respectively. Bacterial GST activity of A. calcoaceticus T32, P. putida SP1 and P. mirabilis V7 was determined to be influenced by different FZD concentrations. Cytotoxicity analysis showed that FZD was degraded to the metabolites with far less cytotoxicity compared to FZD. The inoculation of bacterial strains A. calcoaceticus T32, P. putida SP1 and P. mirabilis V7 into FZD-contained media resulted in a higher degradation efficiency than natural degradation, which indicated the potential application of these strains in treatment of FZD-polluted freshwater or seawater environments. (C) 2012 Elsevier Ltd. All rights reserved. |
| 文章类型 | Article |
| 资助机构 | Chinese Academy of Sciences [KZCX2-EW-206]; National Natural Science Foundation of China (NSFC) [31200041]; Department of Science and Technology of Shandong Province of China [2008GG20005005]; Department of Science and Technology of Yantai City of China [2010235]; Chinese Academy of Sciences |
| 收录类别 | SCI |
| 语种 | 英语 |
| 关键词[WOS] | TRANSFERASES ; HPLC ; FATE |
| 研究领域[WOS] | Biotechnology & Applied Microbiology ; Environmental Sciences & Ecology |
| WOS记录号 | WOS:000316426700007 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.yic.ac.cn/handle/133337/6604 |
| 专题 | 中国科学院海岸带环境过程与生态修复重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Yantai Inst Coastal Zone Res YIC, Key Lab Coastal Zone Environm Proc, Yantai 264003, Peoples R China 2.YICCAS, Shandong Prov Key Lab Coastal Zone Environm Proc, Yantai 264003, Shandong, Peoples R China 3.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China 4.China Agr Univ Yantai, Dept Aquaculture, Yantai 264670, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, Weiwei,Niu, Zongliang,Yin, Kun,et al. Degradation of furazolidone by bacteria Acinetobacter calcoaceticus T32, Pseudomonas putida SP1 and Proteus mirabilis V7[J]. INTERNATIONAL BIODETERIORATION & BIODEGRADATION,2013,77:45-50. |
| APA | Zhang, Weiwei,Niu, Zongliang,Yin, Kun,Liu, Feng,&Chen, Lingxin.(2013).Degradation of furazolidone by bacteria Acinetobacter calcoaceticus T32, Pseudomonas putida SP1 and Proteus mirabilis V7.INTERNATIONAL BIODETERIORATION & BIODEGRADATION,77,45-50. |
| MLA | Zhang, Weiwei,et al."Degradation of furazolidone by bacteria Acinetobacter calcoaceticus T32, Pseudomonas putida SP1 and Proteus mirabilis V7".INTERNATIONAL BIODETERIORATION & BIODEGRADATION 77(2013):45-50. |
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