| Genetic transformation of marine cyanobacterium Synechococcus sp CC9311 (Cyanophyceae) by electroporation | |
Chen Huaxin1; Lin Hanzhi1,2; Jiang Peng1; Li Fuchao1; Qin Song3
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| 发表期刊 | CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY
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| ISSN | 0254-4059 |
| 2013-03-01 | |
| 卷号 | 31期号:2页码:416-420 |
| 关键词 | Chromatic Acclimation Electroporation Genetic Transformation Synechococcus Sp Cc9311 |
| 产权排序 | [Chen Huaxin; Lin Hanzhi; Jiang Peng; Li Fuchao] Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China; [Lin Hanzhi] Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China; [Qin Song] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China |
| 通讯作者 | Jiang, P (reprint author), Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China. [email protected] |
| 作者部门 | 海岸带生物学与生物资源利用所重点实验室 |
| 英文摘要 | Synechococcus sp. CC9311 is a marine cyanobacterium characterized by type IV chromatic acclimation (CA). A genetic transformation system was developed as a first step to elucidate the molecular mechanism of CA. The results show that Synechococcus sp. CC9311 cells were sensitive to four commonly used antibiotics: ampicillin, kanamycin, spectinomycin, and chloramphenicol. An integrative plasmid to disrupt the putative phycoerythrin lyase gene mpeV, using a kanamycin resistance gene as selectable marker, was constructed by recombinant polymerase chain reaction. The plasmid was then transformed into Synechococcus sp. CC9311 via electroporation. High transformation efficiency was achieved at a field strength of 2 kV/cm. DNA analysis showed that mpeV was fully disrupted following challenge of the transformants with a high concentration of kanamycin. In addition, the transformants that displayed poor growth on agar SN medium could be successfully plated on agarose SN medium.; Synechococcus sp. CC9311 is a marine cyanobacterium characterized by type IV chromatic acclimation (CA). A genetic transformation system was developed as a first step to elucidate the molecular mechanism of CA. The results show that Synechococcus sp. CC9311 cells were sensitive to four commonly used antibiotics: ampicillin, kanamycin, spectinomycin, and chloramphenicol. An integrative plasmid to disrupt the putative phycoerythrin lyase gene mpeV, using a kanamycin resistance gene as selectable marker, was constructed by recombinant polymerase chain reaction. The plasmid was then transformed into Synechococcus sp. CC9311 via electroporation. High transformation efficiency was achieved at a field strength of 2 kV/cm. DNA analysis showed that mpeV was fully disrupted following challenge of the transformants with a high concentration of kanamycin. In addition, the transformants that displayed poor growth on agar SN medium could be successfully plated on agarose SN medium. |
| 文章类型 | Article |
| 资助机构 | Key Innovation Project of Institute of Oceanology, Chinese Academy of Sciences [2009-2]; Natural Science Foundation of Shandong Province [2009ZRB02542]; Foundation of Key Laboratory of Marine Bioactive Substance and Modern Analytical Techniques, SOA [MBSMAT-2010-03]; National Natural Science Foundation of China [41276164]; Natural Science Foundation of Jiangsu Province [BK2012650] |
| 收录类别 | SCI |
| 语种 | 英语 |
| 关键词[WOS] | CHROMATIC ADAPTATION ; MANIPULATION ; DIVERSITY ; MOTILITY ; STRAINS ; GENUS |
| 研究领域[WOS] | Marine & Freshwater Biology ; Oceanography |
| WOS记录号 | WOS:000316018300018 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.yic.ac.cn/handle/133337/6440 |
| 专题 | 海岸带生物学与生物资源利用重点实验室_海岸带生物学与生物资源保护实验室 |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China 3.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China |
| 推荐引用方式 GB/T 7714 | Chen Huaxin,Lin Hanzhi,Jiang Peng,et al. Genetic transformation of marine cyanobacterium Synechococcus sp CC9311 (Cyanophyceae) by electroporation[J]. CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY,2013,31(2):416-420. |
| APA | Chen Huaxin,Lin Hanzhi,Jiang Peng,Li Fuchao,&Qin Song.(2013).Genetic transformation of marine cyanobacterium Synechococcus sp CC9311 (Cyanophyceae) by electroporation.CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY,31(2),416-420. |
| MLA | Chen Huaxin,et al."Genetic transformation of marine cyanobacterium Synechococcus sp CC9311 (Cyanophyceae) by electroporation".CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY 31.2(2013):416-420. |
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