| Effects of heavy metals on the expression of a zinc-inducible metallothionein-III gene and antioxidant enzyme activities in Crassostrea gigas | |
Cong, Ming1 ; Wu, Huifeng1; Liu, Xiaoli1,2; Zhao, Jianmin1; Wang, Xuan3; Lv, Jiasen4; Hou, Lin4
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| 发表期刊 | ECOTOXICOLOGY
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| ISSN | 0963-9292 |
| 2012-10-01 | |
| 卷号 | 21期号:7页码:1928-1936 |
| 关键词 | Crassostrea Gigas Metallothionein Cadmium Zinc Antioxidant Enzyme |
| 产权排序 | [Cong, Ming; Wu, Huifeng; Liu, Xiaoli; Zhao, Jianmin] Chinese Acad Sci, Key Lab Coastal Zone Environm Proc, Yantai Inst Coastal Zone Res YIC,YICCAS, Shandong Prov Key Lab Coastal Zone Environm Proc, Yantai 264003, Shandong, Peoples R China; [Liu, Xiaoli] Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China; [Wang, Xuan] Ocean Univ China, Qingdao 266100, Shandong, Peoples R China; [Lv, Jiasen; Hou, Lin] Yantai Univ, Ocean Sch, Yantai 264005, Shandong, Peoples R China |
| 通讯作者 | Wu, HF (reprint author), Chinese Acad Sci, Key Lab Coastal Zone Environm Proc, Yantai Inst Coastal Zone Res YIC,YICCAS, Shandong Prov Key Lab Coastal Zone Environm Proc, Yantai 264003, Shandong, Peoples R China. |
| 作者部门 | 中科院海岸带环境过程与生态修复重点实验室 |
| 英文摘要 | Sequestration by metallothioneins and antioxidant defense are two kinds of important defense mechanisms employed by mollusks to minimize adverse effects caused by heavy metal contaminants in marine environment. In the present study, a novel metallothionein gene, CgMT-III, was cloned from Crassostrea gigas, consisting of eighteen conserved cysteine residues and encoding a MT III-like protein with two tandem beta domains. The expression level of CgMT-III transcript induced by zinc was much higher than that induced by cadmium exposure. It suggested that CgMT-III was perhaps mainly involved in homeostatic control of zinc metabolism, which was distinct from previously identified MTs in C. gigas. Among the tested antioxidant enzymes including superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), SOD and GPx showed varying up-regulations in a tissue-specific manner, while CAT activities were inhibited in both gill and hepatopancreas from C. gigas exposed to heavy metals. It can be inferred that CgMT-III was mainly involved in zinc homeostasis, and CgMT-III gene together with CAT enzyme could be potential biomarkers to indicate heavy metal, especially zinc pollution in marine organisms.; Sequestration by metallothioneins and antioxidant defense are two kinds of important defense mechanisms employed by mollusks to minimize adverse effects caused by heavy metal contaminants in marine environment. In the present study, a novel metallothionein gene, CgMT-III, was cloned from Crassostrea gigas, consisting of eighteen conserved cysteine residues and encoding a MT III-like protein with two tandem beta domains. The expression level of CgMT-III transcript induced by zinc was much higher than that induced by cadmium exposure. It suggested that CgMT-III was perhaps mainly involved in homeostatic control of zinc metabolism, which was distinct from previously identified MTs in C. gigas. Among the tested antioxidant enzymes including superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), SOD and GPx showed varying up-regulations in a tissue-specific manner, while CAT activities were inhibited in both gill and hepatopancreas from C. gigas exposed to heavy metals. It can be inferred that CgMT-III was mainly involved in zinc homeostasis, and CgMT-III gene together with CAT enzyme could be potential biomarkers to indicate heavy metal, especially zinc pollution in marine organisms. |
| 文章类型 | Article |
| 资助机构 | Chinese Academy of Sciences; NSFC [31172388]; SDSFC [ZR2009CZ008] |
| 收录类别 | SCI |
| 语种 | 英语 |
| 关键词[WOS] | MESSENGER-RNA EXPRESSION ; PACIFIC OYSTER ; CADMIUM EXPOSURE ; RUDITAPES-DECUSSATUS ; SUPEROXIDE DISMUTASE ; LIPID-PEROXIDATION ; VIRGINICA GMELIN ; STRESS-PROTEINS ; IN-VITRO ; COPPER |
| 研究领域[WOS] | Environmental Sciences & Ecology ; Toxicology |
| WOS记录号 | WOS:000308235500016 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.yic.ac.cn/handle/133337/5984 |
| 专题 | 中国科学院海岸带环境过程与生态修复重点实验室_污染过程与控制实验室 中国科学院海岸带环境过程与生态修复重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Key Lab Coastal Zone Environm Proc, Yantai Inst Coastal Zone Res YIC,YICCAS, Shandong Prov Key Lab Coastal Zone Environm Proc, Yantai 264003, Shandong, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China 3.Ocean Univ China, Qingdao 266100, Shandong, Peoples R China 4.Yantai Univ, Ocean Sch, Yantai 264005, Shandong, Peoples R China |
| 推荐引用方式 GB/T 7714 | Cong, Ming,Wu, Huifeng,Liu, Xiaoli,et al. Effects of heavy metals on the expression of a zinc-inducible metallothionein-III gene and antioxidant enzyme activities in Crassostrea gigas[J]. ECOTOXICOLOGY,2012,21(7):1928-1936. |
| APA | Cong, Ming.,Wu, Huifeng.,Liu, Xiaoli.,Zhao, Jianmin.,Wang, Xuan.,...&Hou, Lin.(2012).Effects of heavy metals on the expression of a zinc-inducible metallothionein-III gene and antioxidant enzyme activities in Crassostrea gigas.ECOTOXICOLOGY,21(7),1928-1936. |
| MLA | Cong, Ming,et al."Effects of heavy metals on the expression of a zinc-inducible metallothionein-III gene and antioxidant enzyme activities in Crassostrea gigas".ECOTOXICOLOGY 21.7(2012):1928-1936. |
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