| Toxicological responses to acute mercury exposure for three species of Manila clam Ruditapes philippinarum by NMR-based metabolomics | |
Liu, Xiaoli1,2; Zhang, Linbao1,2; You, Liping1,2; Cong, Ming1 ; Zhao, Jianmin1; Wu, Huifeng1; Li, Chenghua1; Liu, Dongyan1; Yu, Junbao1
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| 发表期刊 | ENVIRONMENTAL TOXICOLOGY AND PHARMACOLOGY
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| ISSN | 1382-6689 |
| 2011-03-01 | |
| 卷号 | 31期号:2页码:323-332 |
| 关键词 | Manila Clam Bioindicator Heavy Metal Nmr Metabolomics |
| 产权排序 | [Liu, Xiaoli; Zhang, Linbao; You, Liping; Cong, Ming; Zhao, Jianmin; Wu, Huifeng; Li, Chenghua; Liu, Dongyan; Yu, Junbao] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Zone Environm Proc, CAS,Shandong Prov Key Lab Coastal Zone Environm P, Yantai 264003, Shandong, Peoples R China; [Liu, Xiaoli; Zhang, Linbao; You, Liping] Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China |
| 通讯作者 | Wu, HF, Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Zone Environm Proc, CAS,Shandong Prov Key Lab Coastal Zone Environm P, 17 Chunhui Rd, Yantai 264003, Shandong, Peoples R [email protected] |
| 作者部门 | 污染过程与控制实验室 |
| 英文摘要 | The Manila clam (Ruditapes philippinarum) has been considered a good sentinel species for metal pollution monitoring in estuarine tidal flats. Along the Bohai coast of China, there are dominantly distributed three species of clams (White, Liangdao Red and Zebra in Yantai population) endowed with distinct tolerances to environmental stressors. In this study, adductor muscle samples were collected from both control and acute mercury exposed White, Liangdao Red and Zebra clams, and the extracts were analyzed by NMR-based metabolomics to compare the metabolic profiles and responses to the acute mercury exposure to determine the most sensitive clam species capable of acting as abioindicator for heavy metal pollution monitoring. The major abundant metabolites in the White clam sample were branched-chain amino acids (leucine, isoleucine and valine), lactate, arginine, aspartate, acetylcholine, homarine and ATP/ADP, while the metabolite profile of Zebra clam sample comprised high levels of glutamine, acetoacetate, betaine, taurine and one unidentified metabolite. For the Liangdao Red clam sample, the metabolite profile relatively exhibited high amount of branched-chain amino acids, arginine, glutamate, succinate, acetylcholine, homarine and two unassigned metabolites. After 48h exposure of 20 mu g L-1 Hg2+, the metabolic profiles showed significant differences between three clam species, which included increased lactate, succinate, taurine, acetylcholine, betaine and homarine and decreased alanine, arginine, glutamine, glutamate, acetoacetate, glycine and ATP/ADP in White clam samples, and elevated succinate, taurine and acetylcholine, and declined glutamine, glycine, and aspartate in Liangdao Red clam samples, while the increased branched-chain amino acids, lactate, succinate, acetylcholine and homarine, and reduced alanine, acetoacetate, glycine and taurine were observed in the Zebra clam samples. Overall, our findings showed that White clams could be a preferable bioindicator for the metal pollution monitoring based on the more sensitive metabolic changes in the adductor muscle compared with other two (Liangdao Red and Zebra) clam species. (C) 2010 Elsevier B.V. All rights reserved.; The Manila clam (Ruditapes philippinarum) has been considered a good sentinel species for metal pollution monitoring in estuarine tidal flats. Along the Bohai coast of China, there are dominantly distributed three species of clams (White, Liangdao Red and Zebra in Yantai population) endowed with distinct tolerances to environmental stressors. In this study, adductor muscle samples were collected from both control and acute mercury exposed White, Liangdao Red and Zebra clams, and the extracts were analyzed by NMR-based metabolomics to compare the metabolic profiles and responses to the acute mercury exposure to determine the most sensitive clam species capable of acting as abioindicator for heavy metal pollution monitoring. The major abundant metabolites in the White clam sample were branched-chain amino acids (leucine, isoleucine and valine), lactate, arginine, aspartate, acetylcholine, homarine and ATP/ADP, while the metabolite profile of Zebra clam sample comprised high levels of glutamine, acetoacetate, betaine, taurine and one unidentified metabolite. For the Liangdao Red clam sample, the metabolite profile relatively exhibited high amount of branched-chain amino acids, arginine, glutamate, succinate, acetylcholine, homarine and two unassigned metabolites. After 48h exposure of 20 mu g L-1 Hg2+, the metabolic profiles showed significant differences between three clam species, which included increased lactate, succinate, taurine, acetylcholine, betaine and homarine and decreased alanine, arginine, glutamine, glutamate, acetoacetate, glycine and ATP/ADP in White clam samples, and elevated succinate, taurine and acetylcholine, and declined glutamine, glycine, and aspartate in Liangdao Red clam samples, while the increased branched-chain amino acids, lactate, succinate, acetylcholine and homarine, and reduced alanine, acetoacetate, glycine and taurine were observed in the Zebra clam samples. Overall, our findings showed that White clams could be a preferable bioindicator for the metal pollution monitoring based on the more sensitive metabolic changes in the adductor muscle compared with other two (Liangdao Red and Zebra) clam species. (C) 2010 Elsevier B.V. All rights reserved. |
| 文章类型 | Article |
| 收录类别 | SCI |
| 语种 | 英语 |
| 关键词[WOS] | MAGNETIC-RESONANCE-SPECTROSCOPY ; PATTERN-RECOGNITION ; H-1-NMR METABOLOMICS ; TIDAL FLATS ; METABONOMICS ; EXTRACTION ; TISSUE ; PERFORMANCE ; LA(NO3)(3) ; BIOMARKER |
| 研究领域[WOS] | Environmental Sciences & Ecology ; Pharmacology & Pharmacy ; Toxicology |
| WOS记录号 | WOS:000288636200008 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.yic.ac.cn/handle/133337/4909 |
| 专题 | 中国科学院海岸带环境过程与生态修复重点实验室_污染过程与控制实验室 中国科学院海岸带环境过程与生态修复重点实验室_海岸带环境过程实验室 中国科学院海岸带环境过程与生态修复重点实验室_滨海湿地实验室 中国科学院海岸带环境过程与生态修复重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Zone Environm Proc, CAS,Shandong Prov Key Lab Coastal Zone Environm P, Yantai 264003, Shandong, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Liu, Xiaoli,Zhang, Linbao,You, Liping,et al. Toxicological responses to acute mercury exposure for three species of Manila clam Ruditapes philippinarum by NMR-based metabolomics[J]. ENVIRONMENTAL TOXICOLOGY AND PHARMACOLOGY,2011,31(2):323-332. |
| APA | Liu, Xiaoli.,Zhang, Linbao.,You, Liping.,Cong, Ming.,Zhao, Jianmin.,...&Yu, Junbao.(2011).Toxicological responses to acute mercury exposure for three species of Manila clam Ruditapes philippinarum by NMR-based metabolomics.ENVIRONMENTAL TOXICOLOGY AND PHARMACOLOGY,31(2),323-332. |
| MLA | Liu, Xiaoli,et al."Toxicological responses to acute mercury exposure for three species of Manila clam Ruditapes philippinarum by NMR-based metabolomics".ENVIRONMENTAL TOXICOLOGY AND PHARMACOLOGY 31.2(2011):323-332. |
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