Seagrass Colonization Alters Diversity, Abundance, Taxonomic, and Functional Community Structure of Benthic Microbial Eukaryotes

doi:10.3389/fmicb.2022.901741

	Seagrass Colonization Alters Diversity, Abundance, Taxonomic, and Functional Community Structure of Benthic Microbial Eukaryotes
	Pan, Ying 1; Li, Guihao 2; Su, Lei3 ; Zheng, Pengfei 3; Wang, Yaping 2; Shen, Zhuo 2; Chen, Zigui 4; Han, Qiuying5 ; Gong, Jun2,6,7
发表期刊	FRONTIERS IN MICROBIOLOGY
	2022-06-13
卷号	13 页码:15
关键词	community structure belowground diversity protist parasite high throughput sequencing functional composition
DOI	10.3389/fmicb.2022.901741
通讯作者	Shen, Zhuo([email protected]) ; Gong, Jun([email protected])
英文摘要	Seagrass form high productive ecosystems in coastal environments. However, the effects of these coastal plants on the structure and function of the belowground eukaryotic microbiome remain elusive. In this study, we characterized the community of microbial eukaryotes (microeukaryotes) in both vegetated and unvegetated sediments using 18S rRNA gene amplicon sequencing and quantitative PCR. Analysis of sequencing data showed that the eelgrass (Zostera marina) colonization decreased the alpha diversity indices of benthic microeukaryotes. Apicomplexa represented an average of 83% of reads across all samples, with a higher proportion at the vegetated sites. The taxonomic community structure was significantly different between these two types of sediments, for which the concentration of NH4+ in sediment porewater and salinity could account. Phylogenetic analyses of long 18S rRNA genes (around 1,030 bp) indicated these apicomplexan parasites are closely related to gregarine Lecudina polymorpha. Determination of 18S rRNA gene abundances provided evidence that the eelgrass markedly promoted the biomass of the gregarine and all microeukaryotes in the seagrass-colonized sediments and confirmed that the gregarine was hosted by a polychaete species. Significantly higher gene abundances of heterotrophs and mixotrophs were found at the vegetated sites, which could be explained by the finer sediments and short supply of dissolved inorganic nitrogen, respectively. The pigmented protists were more abundant in 18S rRNA gene copies at the lower and higher pH levels than at the intermediate. Nevertheless, the fractions of heterotrophs and phototrophs in the community were significantly related to porewater N:P ratio. These results indicate that seagrass colonization significantly induces an increase in overall biomass and a decrease in diversity of benthic microeukaryotes, making them more heterotrophic. This study also highlights that the hotspot of eukaryotic parasites could be linked with the high productivity of a natural ecosystem.
资助机构	National Natural Science Foundation of China ; Natural Science Foundation of Jiangsu Province ; Innovation Group Project of Southern Marine Science and Engineering Guangdong Laboratory (Zhuhai) ; CAS Frontiers in Scientific Research Programs, Projects for Young Top-notch Talents
收录类别	SCI
语种	英语
关键词[WOS]	SWAN LAKE ; MARINE ; MICROPHYTOBENTHOS ; SEQUENCES ; SEDIMENTS ; AMMONIUM ; HABITAT ; COAST
研究领域[WOS]	Microbiology
WOS记录号	WOS:000816863300001
引用统计	被引频次：6[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.yic.ac.cn/handle/133337/37388
专题	中国科学院烟台海岸带研究所知识产出
通讯作者	Shen, Zhuo; Gong, Jun
作者单位	1.Sun Yat Sen Univ, Sch Ecol, Shenzhen, Peoples R China 2.Sun Yat Sen Univ, Sch Marine Sci, Lab Microbial Ecol & Matter Cycle, Zhuhai, Peoples R China 3.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai, Peoples R China 4.Chinese Univ Hong Kong, Dept Microbiol, Hong Kong, Peoples R China 5.Hainan Trop Ocean Univ, Coll Ecol & Environm, Sanya, Peoples R China 6.Southern Marine Sci & Engn Guangdong Lab, Zhuhai, Peoples R China 7.Guangdong Prov Key Lab Marine Resources & Coastal, Guangzhou, Peoples R China
推荐引用方式 GB/T 7714	Pan, Ying,Li, Guihao,Su, Lei,et al. Seagrass Colonization Alters Diversity, Abundance, Taxonomic, and Functional Community Structure of Benthic Microbial Eukaryotes[J]. FRONTIERS IN MICROBIOLOGY,2022,13:15.
APA	Pan, Ying.,Li, Guihao.,Su, Lei.,Zheng, Pengfei.,Wang, Yaping.,...&Gong, Jun.(2022).Seagrass Colonization Alters Diversity, Abundance, Taxonomic, and Functional Community Structure of Benthic Microbial Eukaryotes.FRONTIERS IN MICROBIOLOGY,13,15.
MLA	Pan, Ying,et al."Seagrass Colonization Alters Diversity, Abundance, Taxonomic, and Functional Community Structure of Benthic Microbial Eukaryotes".FRONTIERS IN MICROBIOLOGY 13(2022):15.