Molecular characterization, expression and antibacterial function of a macin, HdMac, from <i>Haliotis discus hannai</i>

doi:10.1016/j.jip.2024.108113

	Molecular characterization, expression and antibacterial function of a macin, HdMac, from Haliotis discus hannai
	Jiao, Chunli 1; Ruan, Jian 1; Sun, Wei 2; Zhang, Xinze 2; Liu, Xiaobo 2; Sun, Guodong 4; Liu, Caili 2; Sun, Chunxiao 2; Tian, Xiuhui 2; Yang, Dinglong3 ; Chen, Lizhu 2; Wang, Zhongquan 2
发表期刊	JOURNAL OF INVERTEBRATE PATHOLOGY
ISSN	0022-2011
	2024-06-01
卷号	204 页码:8
关键词	Haliotis discus hannai Macin Antibacterial peptides Immune responses
DOI	10.1016/j.jip.2024.108113
通讯作者	Chen, Lizhu([email protected]) ; Wang, Zhongquan([email protected])
英文摘要	Macins are a family of antimicrobial peptides, which play multiple roles in the elimination of invading pathogens. In the present study, a macin was cloned and characterized from Pacific abalone Haliotis discus hannai (Designated as HdMac). Analysis of the conserved domain suggested that HdMac was a new member of the macin family. In non-stimulated abalones, HdMac transcripts were constitutively expressed in all five tested tissues, especially in hemocytes. After Vibrio harveyi stimulation, the expression of HdMac mRNA in hemocytes was significantly up-regulated at 12 hr (P < 0.01). RNAi-mediated knockdown of HdMac transcripts affected the survival rates of abalone against V. harveyi. Moreover, recombinant protein of HdMac (rHdMac) exhibited high antibacterial activities against invading bacteria, especially for Vibrio anguillarum. In addition, rHdMac possessed binding activities towards glucan, lipopolysaccharides (LPS), and peptidoglycan (PGN), but not chitin in vitro. Membrane integrity analysis revealed that rHdMac could increase the membrane permeability of bacteria. Meanwhile, both the phagocytosis and chemotaxis ability of hemocytes could be significantly enhanced by rHdMac. Overall, the results showed that HdMac could function as a versatile molecule involved in immune responses of H. discus hannai.
资助机构	Natural Science Foundation of Shandong Province ; Shandong Key Laboratory of Marine Ecological Restoration Open Fund ; National Natural Science Foundation of China
收录类别	SCI
语种	英语
关键词[WOS]	ANTIMICROBIAL PEPTIDES ; INNATE IMMUNITY ; MECHANISMS ; CATHELICIDINS ; DEFENSINS
研究领域[WOS]	Zoology
WOS记录号	WOS:001232050100001
引用统计
文献类型	期刊论文
条目标识符	http://ir.yic.ac.cn/handle/133337/36033
专题	海岸带生物学与生物资源利用重点实验室海岸带生物学与生物资源利用重点实验室_海岸带生物资源高效利用研究与发展中心
通讯作者	Chen, Lizhu; Wang, Zhongquan
作者单位	1.Yantai Ctr Food & Drug Control, Yantai 264003, Peoples R China 2.Shandong Marine Resource & Environm Res Inst, Shandong Key Lab Marine Ecol Restorat, Yantai 264006, Peoples R China 3.Chinese Acad Sci, Muping Coastal Environm Res Stn, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China 4.Tianjin Xiqing Dist Agr & Rural Dev Serv Ctr, Tianjin 300380, Peoples R China
推荐引用方式 GB/T 7714	Jiao, Chunli,Ruan, Jian,Sun, Wei,et al. Molecular characterization, expression and antibacterial function of a macin, HdMac, from Haliotis discus hannai[J]. JOURNAL OF INVERTEBRATE PATHOLOGY,2024,204:8.
APA	Jiao, Chunli.,Ruan, Jian.,Sun, Wei.,Zhang, Xinze.,Liu, Xiaobo.,...&Wang, Zhongquan.(2024).Molecular characterization, expression and antibacterial function of a macin, HdMac, from Haliotis discus hannai.JOURNAL OF INVERTEBRATE PATHOLOGY,204,8.
MLA	Jiao, Chunli,et al."Molecular characterization, expression and antibacterial function of a macin, HdMac, from Haliotis discus hannai".JOURNAL OF INVERTEBRATE PATHOLOGY 204(2024):8.