N 2 O emission in temperate seagrass meadows: Fluxes, pathway and molecular mechanism

doi:10.1016/j.marenvres.2024.106542

	N 2 O emission in temperate seagrass meadows: Fluxes, pathway and molecular mechanism
	He, Qianling 1; Qin, Huawei 3; Yang, Lin 1; Tan, Wenwen 2; Ji, Daode 1; Zhang, Jianbai 4; Zhang, Xiaoli2
发表期刊	MARINE ENVIRONMENTAL RESEARCH
ISSN	0141-1136
	2024-06-01
卷号	198 页码:13
关键词	N2O emission Seagrass meadow Heterotrophic nitrification Metagenomic analysis Isotopic tracing
DOI	10.1016/j.marenvres.2024.106542
通讯作者	Zhang, Jianbai([email protected]) ; Zhang, Xiaoli([email protected])
英文摘要	Seagrass meadows act as filters for nitrogen in coastal areas, but whether they are a source or sink for N 2 O has been still controversy. Additionally, the production pathways of N 2 O as well as the microbial driving mechanism in seagrass meadows are seldom reported. In this study, the air-sea fluxes, sediment release potential, and production pathway of N 2 O in a temperate Zostera marina and Z. japonica mixed meadow were investigated by using gas chromatography and 15 N isotopic tracing methods. The qPCR and metagenome sequencing were used to compare the difference in functional gene abundance and expression between seagrass vegetated and nongrass sediments. The results showed that the N 2 O air-sea fluxes in the meadow ranged from -1.97 to -1.77 nmol m - 2 h - 1 , which was slightly lower in the seagrass region than in the adjacent bare region. Seagrass sediment N 2 O release potential dramatically increased after warming and nitrogen enrichment treatments. Heterotrophic nitrification was firstly investigated in seagrass meadows, and the process (26.80% -62.41%) and denitrification (37.55% -72.83%) contributed significantly to N 2 O production in the meadow, affected deeply by sediment organic content, while the contribution of autotrophic nitrification can be neglected. Compared with the bare sediments, the ammonia monooxygenase genes amoA , amoB and amoC , and nitrite oxidoreductase genes nxrA and nxrB , as well as nitrite reductase gene nirS and nitric oxide reductase gene norB were down-regulated, while the nitrous oxide reductase gene nosZ was up-regulated in the seagrass sediments, explaining less N 2 O emission in seagrass regions from the perspective of molecular. The nosZII -bearing bacteria like Bacteroidia , Polyangia , Anaerolineae , and Verrucomicrobiae could play important roles in N 2 O reduction in the seagrass meadow. The result is of great significance for highlighting the ability of seagrass meadows to mitigate climate changes.
资助机构	National Key R&D Program of China ; National Natural Science Foundation of China ; Science and Technology Development Program of Yantai ; Seed Project of Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences ; Innovation Youth Fund Project of the Marine Telemetry Technology Innovation Center of the Ministry of Natural Resources
收录类别	SCI
语种	英语
关键词[WOS]	EELGRASS ZOSTERA-MARINA ; NITROUS-OXIDE ; HETEROTROPHIC NITRIFICATION ; SHANDONG PENINSULA ; N2O EMISSION ; GAS-EXCHANGE ; FOREST SOIL ; FRESH-WATER ; WIND-SPEED ; DENITRIFICATION
研究领域[WOS]	Environmental Sciences & Ecology ; Marine & Freshwater Biology ; Toxicology
WOS记录号	WOS:001246444200001
引用统计
文献类型	期刊论文
条目标识符	http://ir.yic.ac.cn/handle/133337/35918
专题	海岸带生物学与生物资源利用重点实验室海岸带生物学与生物资源利用重点实验室_海岸带生物资源高效利用研究与发展中心
通讯作者	Zhang, Jianbai; Zhang, Xiaoli
作者单位	1.Yantai Univ, Sch Ocean, Yantai 264005, Peoples R China 2.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Environm Proc & Ecol Remediat, Yantai 264003, Peoples R China 3.Shandong Marine Resource & Environm Res Inst, Yantai 264006, Peoples R China 4.Yantai Marine Econ Res Inst, Yantai 264000, Peoples R China
推荐引用方式 GB/T 7714	He, Qianling,Qin, Huawei,Yang, Lin,et al. N 2 O emission in temperate seagrass meadows: Fluxes, pathway and molecular mechanism[J]. MARINE ENVIRONMENTAL RESEARCH,2024,198:13.
APA	He, Qianling.,Qin, Huawei.,Yang, Lin.,Tan, Wenwen.,Ji, Daode.,...&Zhang, Xiaoli.(2024).N 2 O emission in temperate seagrass meadows: Fluxes, pathway and molecular mechanism.MARINE ENVIRONMENTAL RESEARCH,198,13.
MLA	He, Qianling,et al."N 2 O emission in temperate seagrass meadows: Fluxes, pathway and molecular mechanism".MARINE ENVIRONMENTAL RESEARCH 198(2024):13.