| An electrochemical amplification immunoassay using biocatalytic metal deposition coupled with anodic stripping voltammetric detection | |
Chen, Zhao-Peng1,2 ; Peng, Zhao-Feng1; Jiang, Jian-Hui1; Zhang, Xiao-Bing1; Shen, Guo-Li1; Yu, Ru-Qin1
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| 发表期刊 | SENSORS AND ACTUATORS B-CHEMICAL
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| ISSN | 0925-4005 |
| 2008-01-29 | |
| 卷号 | 129期号:1页码:146-151 |
| 关键词 | Electrochemical Immunoassay Anodic Stripping Voltammmetry Biocatalytic Metal Deposition |
| 产权排序 | Hunan Univ, Coll Chem & Chem Engn, State Key Lab Chemo Biosensing & Chemometr; Chinese Acad Sci, Dept Environm Chem, Yantai Inst Coastal Zone Res Sustainable Dev |
| 通讯作者 | Yu, RQ, Hunan Univ, Coll Chem & Chem Engn, State Key Lab Chemo Biosensing & Chemometr, Changsha 410082, Peoples R China |
| 作者部门 | 环境化学实验室 |
| 英文摘要 | An electrochemical amplification immunoassay is reported using biocatalytic metal deposition coupled with anodic stripping voltammetric detection. In this method, the captured antibody was first immobilized onto a gold electrode via a self-assembled layer. After a sandwich immunoreaction, alkaline phosphatase-labeled antibody was bound to the gold electrode. The alkaline phosphatase on the electrode catalyzes the hydrolysis of ascorbic acid 2-phosphate to produce ascorbic acid. The latter, in turn, reduced silver ions on the electrode surface, leading to the deposition of silver onto the protein-modified electrode surface. The deposited metal was electrochemically stripped into solution and then measured by anodic stripping voltammmetry. Compared with the direct voltammetric detection of ascorbic acid, anodic stripping voltammetric detection of metal ions is more sensitive. For the amount of deposited silver relates to the amount of enzyme-generated ascorbic acid, which was controlled by the amount of enzyme bound on the electrode surface, the stripping current signal reflects the amount of target protein, achieving a linearly relationship in the range from 5 to 1000 ng mL(-1) in a logarithmic plot with a detection limit of 2.2 ng mL(-1). The utilization of the high biocatalytic activity of enzyme and the sensitive anodic stripping voltammetry to detect metal ions dramatically enhanced the sensitivity in immunoassay. (C) 2007 Elsevier B.V. All rights reserved. |
| 文章类型 | Article |
| 收录类别 | SCI |
| 语种 | 英语 |
| 关键词[WOS] | GOLD NANOPARTICLE LABEL ; DNA HYBRIDIZATION ; AMPEROMETRIC IMMUNOSENSORS ; HORSERADISH-PEROXIDASE ; ENZYME-IMMUNOASSAY ; LIPOSOMES ; TRANSDUCTION ; ELECTRODES |
| 研究领域[WOS] | Chemistry ; Electrochemistry ; Instruments & Instrumentation |
| WOS记录号 | WOS:000253172500023 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.yic.ac.cn/handle/133337/3455 |
| 专题 | 中国科学院海岸带环境过程与生态修复重点实验室_海岸带环境工程技术研究与发展中心 中国科学院海岸带环境过程与生态修复重点实验室_海岸带环境过程实验室 |
| 作者单位 | 1.Hunan Univ, Coll Chem & Chem Engn, State Key Lab Chemo Biosensing & Chemometr, Changsha 410082, Peoples R China 2.Chinese Acad Sci, Dept Environm Chem, Yantai Inst Coastal Zone Res Sustainable Dev, Yantai 264003, Peoples R China |
| 推荐引用方式 GB/T 7714 | Chen, Zhao-Peng,Peng, Zhao-Feng,Jiang, Jian-Hui,et al. An electrochemical amplification immunoassay using biocatalytic metal deposition coupled with anodic stripping voltammetric detection[J]. SENSORS AND ACTUATORS B-CHEMICAL,2008,129(1):146-151. |
| APA | Chen, Zhao-Peng,Peng, Zhao-Feng,Jiang, Jian-Hui,Zhang, Xiao-Bing,Shen, Guo-Li,&Yu, Ru-Qin.(2008).An electrochemical amplification immunoassay using biocatalytic metal deposition coupled with anodic stripping voltammetric detection.SENSORS AND ACTUATORS B-CHEMICAL,129(1),146-151. |
| MLA | Chen, Zhao-Peng,et al."An electrochemical amplification immunoassay using biocatalytic metal deposition coupled with anodic stripping voltammetric detection".SENSORS AND ACTUATORS B-CHEMICAL 129.1(2008):146-151. |
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