The invention relates to a pesticide field, and concretely relates to an alga endophytic fungi diterpenoid alkaloid compound, a preparation method thereof and an application thereof. The preparation method comprises the following steps : Aspergillus oryzae cf-2 is inoculated into a medium and ferments statically, a fermentation broth is extracted and condensed through ethyl acetate to obtain a first condensate, mycelium is extracted with an organic solvent and is extracted and condensed with ethyl acetate to obtain a second condensate which is combined with the first condensate to obtain a crude extract; the crude extract is treated with silica column chromatography and is treated with gradient elution by an organic solvent, and an eluent is collected and is detected through thin layer chromatography (TLC); and an eluent component obtained through gradient elution with an eluent volume ratio of 10 to 15 : 1 is collected, and is treated with gel column chromatography, silicate column chromatography and the TLC for separation and purification to obtain the target compound. The alga endophytic fungi diterpenoid alkaloid compound with a concentration of 100 g/ml has a fatality of 61.9% to Artemia species according to insecticidal activity tests.
; 乔明锋
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