本发明涉及基因工程技术领域，具体涉及一种菊芋抗虫基因及其植物表达载体构建方法和应用。菊芋抗虫(JaSPI)基因碱基序列如SEQ ID NO：1所示；或，与SEQ ID NO：1所示碱基序列同源性在85％以上的基因。JaSPI编码的氨基酸序列如SEQ ID NO：2所示。本发明提供的JaSPI基因及其表达载体能用于植物遗传转化，提高植物抗虫性及抗逆性。另外本发明提供的JaSPI基因能够用于体外表达，为获得新的生物杀虫剂、辅助Bt等生物制剂共同预防农业害虫提供了新的途径。
其他摘要
The invention specifically relates to construction methods and application of an insect-resistant gene of jerusalem artichoke and an expression vector thereof, belonging to the field of genetic engineering technology. The base sequence of the insect-resistant gene (JaSPI) of jerusalem artichoke is as shown in SEQ ID No. 1; or the insect-resistant gene (JaSPI) is a gene with a base sequence 85% or more homological to the base sequence as shown in SEQ ID No. 1. The amino acid sequence coded by JaSPI is as shown in SEQ ID No. 2. The JaSPI gene and the expression vector thereof can be applied to genetic transformation of plants for improvement of insect resistance and stress resistance of plants. Moreover, the JaSPI gene provided by the invention can be used for in-vitro expression, so a novel approach is provided for development of novel biological preparations like biopesticides and auxiliary Bt for co-prevention of agricultural insects.
; 秦松
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