The invention belongs to the technical field of material science and engineering and micro-fluidic chips, and particularly relates to a micro-fluidic paper chip of for detecting a liquid sample based on ion imprinting-coated quantum dots and a preparation method thereof. The micro-fluidic paper chip for detecting the liquid sample is characterized in that the structure of the ion imprinting layer-coated quantum dots is fixedly arranged on a glass fiber surface with a wax-spraying Y-shaped micro-fluidic channel by an amido bond forming method and a sol-gel method, so as to obtain the micro-fluidic paper chip of the detection liquid sample. The micro-fluidic paper chip for detecting the liquid sample has the advantages that the rapidity, high efficiency, portability, economy, high sensitivity and the like are realized; a new strategy for simultaneously detecting multiple heavy metal ions is provided, and the related study of the micro-fluidic chips is enriched.
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