The invention relates to the technical field of in-situ advanced treatment purification of sewage, and in particular relates to a method for in-situ aerobic denitrification treatment purification of aquaculture sewage. The method comprises the following steps : fecal residual bait in a culture pond is taken out and treated, the treated bait, composite strains, antibiotics, a high-carbon alcohol fatty acid ester complex and a microbial flocculating agent are mixed for fermentation cultivation, and a precipitate obtained by the cultivation is a microbial flocculation precipitate with a heterotrophic nitrification-aerobic denitrification function; an organic carbon source is added into the fermentation broth obtained by the cultivation, uniform mixing is performed, and the mixed material is sprayed to the surface of feed of aquaculture animals to obtain heterotrophic nitrification-aerobic denitrifying bacteria spraying feed; the obtained microbial flocculation precipitate and the sprayingfeed are mixed, and the mixed material is thrown into the culture pond to be treated for eating of the aquaculture animals in the culture pond; and a water body in the culture pond is regulated, composite strains are added into the culture water body, and therefore active bacterium cluster floc with the heterotrophic nitrification-aerobic denitrification function capable of purifying the water body is formed in the water body. According to the method provided by the invention, the above obtained substances are utilized, so that in-situ aerobic denitrification advanced treatment of the water body in the culture pond is realized, and organic matter, ammonia nitrogen, nitrite nitrogen and nitrate nitrogen are removed.
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