The invention relates to agriculture, daily chemicals and food industries, in particular to a preparation method of several anionic polysaccharide derivatives and application of the prepared polysaccharide derivatives. Reacting carboxyl-containing saccharide compound with betaine solution or 2, 3-epoxypropyl trimethyl ammonium chloride solution to obtain anionic polysaccharide derivative; Or, the carboxyl-containing saccharide compound is used as a raw material to react with a betaine solution, and after the reaction, the carboxyl-containing saccharide compound reacts with a 2, 3-epoxypropyl trimethyl ammonium chloride solution reactant to obtain the anionic polysaccharide derivative. The preparation method of the anionic polysaccharide derivative comprises the following steps of : 1, preparing the anionic polysaccharide derivative; and 2, 3-epoxypropyl trimethyl ammonium chloride solution reactant. The method has the advantages that the raw materials are cheap and easy to obtain, the experiment operation process is simple, the reaction conditions are nontoxic, and large-scale industrialization is facilitated. Researches prove that the anionic polysaccharide derivatives have good moisture absorption and retention activities and certain regularity, and can be widely applied to the cosmetic and food industries.
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