iTRAQ-Based Quantitative Proteomic Analysis Reveals Toxicity Mechanisms in Chlamys farreri Exposed to Okadaic Acid

doi:10.3389/fmars.2021.792050

	iTRAQ-Based Quantitative Proteomic Analysis Reveals Toxicity Mechanisms in Chlamys farreri Exposed to Okadaic Acid
	Wang, Xin 1,2,3; Wang, Dan 1,2,3; Zhang, Tianyu 1,2,3; Zhang, Qianqian1,2,4 ; Zhao, Jianmin1,2,4
发表期刊	FRONTIERS IN MARINE SCIENCE
	2021-12-08
卷号	8 页码:12
关键词	okadaic acid Chlamys farreri oxidative stress detoxification genes iTRAQ
DOI	10.3389/fmars.2021.792050
通讯作者	Zhang, Qianqian([email protected]) ; Zhao, Jianmin([email protected])
英文摘要	Okadaic acid (OA), produced by dinoflagellates during harmful algal blooms, is a principal diarrhetic shellfish poisoning toxin. This toxin poses a potential threat to bivalves with economic values. To better understand the toxicity mechanism of OA to bivalves, in this study, oxidative stress biomarkers (superoxide dismutase, SOD; catalase, CAT; glutathione S-transferase, GST; malondialdehyde, MDA) and the expression of detoxification genes (heat shock protein 70, HSP70; heat shock protein 90, HSP90; cytochrome P450, CYP450) were assessed in the gills of scallops Chlamys farreri after 24 h, 48 h and 96 h exposure to OA. In addition, the digestive glands of scallops exposed to OA for 96 h were dissected for an iTRAQ based quantitative proteomic analysis. The results of OA exposure experiments showed that OA induces oxidative stress and significant enhancement of the expression of detoxification genes in scallops. The proteomics analysis revealed that 159 proteins altered remarkably in OA-treated scallops, and these proteins were involved in phagosomes, regulation of actin cytoskeleton, adherens junction, tight junction, and focal adhesion. Amino acid biosynthesis, carbon metabolism, pentose phosphate pathway, fructose and mannose metabolism in the digestive glands were also significantly impacted. Our data shed new insights on the molecular responses and toxicity mechanisms of C. farreri to OA.
资助机构	Strategic Priority Research Program of the Chinese Academy of Sciences ; National Key Research and Development Program of China ; Youth Innovation Promotion Association, Chinese Academy of Sciences
收录类别	SCI
语种	英语
关键词[WOS]	MYTILUS-GALLOPROVINCIALIS ; OXIDATIVE STRESS ; GENE-EXPRESSION ; ALGAL BLOOMS ; SUPEROXIDE-DISMUTASE ; ANTIOXIDANT ENZYMES ; PROROCENTRUM-LIMA ; MARINE TOXINS ; UNIO-TUMIDUS ; F-ACTIN
研究领域[WOS]	Environmental Sciences & Ecology ; Marine & Freshwater Biology
WOS记录号	WOS:000732816700001
引用统计	被引频次：3[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.yic.ac.cn/handle/133337/31532
专题	支撑部门_中国科学院牟平海岸带环境综合试验站海岸带生物学与生物资源利用重点实验室_海岸带生物学与生物资源保护实验室海岸带生物学与生物资源利用重点实验室_海岸带生物资源高效利用研究与发展中心海岸带生物学与生物资源利用重点实验室
通讯作者	Zhang, Qianqian; Zhao, Jianmin
作者单位	1.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Muping Coastal Environm Res Stn, Yantai, Peoples R China 2.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Biol & Biol Resources Utilizat, Yantai, Peoples R China 3.Univ Chinese Acad Sci, Coll Resources & Environm, Beijing, Peoples R China 4.Chinese Acad Sci, Ctr Ocean Mega Sci, Qingdao, Peoples R China
推荐引用方式 GB/T 7714	Wang, Xin,Wang, Dan,Zhang, Tianyu,et al. iTRAQ-Based Quantitative Proteomic Analysis Reveals Toxicity Mechanisms in Chlamys farreri Exposed to Okadaic Acid[J]. FRONTIERS IN MARINE SCIENCE,2021,8:12.
APA	Wang, Xin,Wang, Dan,Zhang, Tianyu,Zhang, Qianqian,&Zhao, Jianmin.(2021).iTRAQ-Based Quantitative Proteomic Analysis Reveals Toxicity Mechanisms in Chlamys farreri Exposed to Okadaic Acid.FRONTIERS IN MARINE SCIENCE,8,12.
MLA	Wang, Xin,et al."iTRAQ-Based Quantitative Proteomic Analysis Reveals Toxicity Mechanisms in Chlamys farreri Exposed to Okadaic Acid".FRONTIERS IN MARINE SCIENCE 8(2021):12.