Institutional Repository of Key Laboratory of Coastal Zone Environmental Processes, Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences (KLCEP)
| Proteomic analysis revealed gender-specific responses of mussels (Mytilus galloprovincialis) to trichloropropyl phosphate (TCPP) exposure | |
Zhong, Mingyu1; Wu, Huifeng1,2,3 ; Li, Fei1,3; Shan, Xiujuan2; Ji, Chenglong1,2,3
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| 发表期刊 | ENVIRONMENTAL POLLUTION
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| ISSN | 0269-7491 |
| 2020-12-01 | |
| 卷号 | 267页码:11 |
| 关键词 | Trichloropropyl phosphate (TCPP) Mytilus galloprovincialis Gender-specific effects iTRAQ Proteomics |
| DOI | 10.1016/j.envpol.2020.115537 |
| 通讯作者 | Wu, Huifeng([email protected]) |
| 英文摘要 | Trichloropropyl phosphate (TCPP) is a halogenated organophosphate ester that is widely used as flame retardants and plasticizers. In this study, gender-specific accumulation and responses in mussel Mytilus galloprovincialis to TCPP exposure were focused and highlighted. After TCPP (100 nmol L-1) exposure for 42 days, male mussels showed similar average bioaccumulation (37.14 6.09 nmol g(-1) fat weight (fw)) of TCPP with that in female mussels (32.28 +/- 4.49 nmol g(-1) fw). Proteomic analysis identified 219 differentially expressed proteins (DEPs) between male and female mussels in control group. There were 52 and 54 DEPs induced by TCPP in male and female mussels, respectively. Interestingly, gender-specific DEPs included 37 and 41 DEPs induced by TCPP in male and female mussels, respectively. The proteomic differences between male and female mussels were related to protein synthesis and degradation, energy metabolism, and functions of cytoskeleton and motor proteins. TCPP influenced protein synthesis, energy metabolism, cytoskeleton functions, immunity, and reproduction in both male and female mussels. Protein-protein interaction (PPI) networks indicated that protein synthesis and energy metabolism were the main biological processes influenced by TCPP. However, DEPs involved in these processes and their interaction patterns were quite different between male and female mussels. Basically, twelve ribosome DEPs which directly or indirectly interacted were found in protein synthesis in TCPPexposed male mussels, while only 3 ribosome DEPs (not interacted) in TCPP-exposed female mussels. In energy metabolism, only 4 DEPs (with the relatively simple interaction pattern) mainly resided in fatty acid metabolism, butanoate/propanoate metabolism and glucose metabolism were discovered in TCPPexposed male mussels, and more DEPs (with multiple interactions) functioned in TCA cycle and pyruvate/glyoxylate/dicarboxylate metabolism were found in TCCP-exposed female mussels. Taken together, TCPP induced gender-specific toxicological effects in mussels, which may shed new lights on further understanding the toxicological mechanisms of TCPP in aquatic organisms. (c) 2020 Elsevier Ltd. All rights reserved. |
| 资助机构 | National Natural Science Foundation of China ; Marine S&T Fund of Shandong Province for Pilot National Laboratory for Marine Science and Technology (Qingdao) ; Youth Innovation Promotion Association CAS |
| 收录类别 | SCI |
| 语种 | 英语 |
| 关键词[WOS] | ORGANOPHOSPHATE FLAME RETARDANTS ; PHYSICAL-CHEMICAL PROPERTIES ; MESSENGER-RNA EXPRESSION ; SPATIAL-DISTRIBUTION ; YELLOW SEAS ; BOHAI SEA ; PLASTICIZERS ; PROTEIN ; CHROMATOGRAPHY ; TOXICITY |
| 研究领域[WOS] | Environmental Sciences & Ecology |
| WOS记录号 | WOS:000593966400007 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.yic.ac.cn/handle/133337/27529 |
| 专题 | 中国科学院海岸带环境过程与生态修复重点实验室 中国科学院海岸带环境过程与生态修复重点实验室_海岸带环境过程实验室 |
| 通讯作者 | Wu, Huifeng |
| 作者单位 | 1.Chinese Acad Sci, Shandong Key Lab Coastal Environm Proc, CAS Key Lab Coastal Environm Proc & Ecol Remediat, Yantai Inst Coastal Zone Res YIC,YICCAS, Yantai 264003, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Fisheries Sci & Food Prod Proc, Qingdao 266237, Peoples R China 3.Chinese Acad Sci, Ctr Ocean Mega Sci, Qingdao 266071, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhong, Mingyu,Wu, Huifeng,Li, Fei,et al. Proteomic analysis revealed gender-specific responses of mussels (Mytilus galloprovincialis) to trichloropropyl phosphate (TCPP) exposure[J]. ENVIRONMENTAL POLLUTION,2020,267:11. |
| APA | Zhong, Mingyu,Wu, Huifeng,Li, Fei,Shan, Xiujuan,&Ji, Chenglong.(2020).Proteomic analysis revealed gender-specific responses of mussels (Mytilus galloprovincialis) to trichloropropyl phosphate (TCPP) exposure.ENVIRONMENTAL POLLUTION,267,11. |
| MLA | Zhong, Mingyu,et al."Proteomic analysis revealed gender-specific responses of mussels (Mytilus galloprovincialis) to trichloropropyl phosphate (TCPP) exposure".ENVIRONMENTAL POLLUTION 267(2020):11. |
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