Global characterization of dose-dependent effects of cadmium in clam Ruditapes philippinarum

doi:10.1016/j.envpol.2021.116443

	Global characterization of dose-dependent effects of cadmium in clam Ruditapes philippinarum
	Zhan, Junfei 1,2,5; Wang, Shuang 1,2,5; Li, Fei1,2,4 ; Ji, Chenglong1,2,3,4 ; Wu, Huifeng1,2,3,4
发表期刊	ENVIRONMENTAL POLLUTION
ISSN	0269-7491
	2021-03-15
卷号	273 页码:9
关键词	Benchmark dose Cadmium Dose-dependent effects Ruditapes philippinarum Transcriptomics
DOI	10.1016/j.envpol.2021.116443
通讯作者	Wu, Huifeng([email protected])
英文摘要	Cadmium (Cd) is being frequently detected in marine organisms. However, dose-dependent effects of Cd challenged unraveling the toxicological mechanisms of Cd to marine organisms and developing biomarkers. Here, the dose-dependent effects of Cd on clams Ruditapes philippinarum following exposure to 5 doses of Cd (3, 9, 27, 81, 243 mg/L) were investigated using benchmark dose (BMD) method. By model fitting, calculation of BMD values was performed on transcriptomic profiles, metals concentrations, and antioxidant indices. Cd exposure induced not only significant Cd accumulation in clams, but also marked alterations of essential metals such as Ca, Cu, Zn, Mn, and Fe. Gene regulation posed little influence on essential metal homeostasis, indicated by poor enrichment of differentially expressed genes (DEGs) associated with metal binding and metal transport in lower concentrations of Cd-treated groups. BMD analysis on biological processes and pathways showed that peptide cross-linking was the most sensitive biological process to Cd exposure, followed by focal adhesion, ubiquitin mediated proteolysis, and apoptosis. Occurrence of apoptosis was also confirmed by TUENL-positive staining in gills and hepatopancreas of clams treated with Cd. Furthermore, many DEGs, such as transglutaminases (TGs), metallothionein (MT), STEAP2-like and laccase, which presented linear or monotonic curves and relatively low BMD values, were potentially preferable biomarkers in clams to Cd. Overall, BMD analysis on transcriptomic profiles, metals concentrations and biochemical endpoints unraveled the sensitiveness of key events in response to Cd treatments, which provided new insights in exploring the toxicological mechanisms of Cd in clams as well as biomarker selection. (C) 2021 Elsevier Ltd. All rights reserved.
资助机构	National Natural Science Foundation of China ; Young Taishan Scholars Program of Shandong Province
收录类别	SCI
语种	英语
研究领域[WOS]	Environmental Sciences & Ecology
WOS记录号	WOS:000625376600033
引用统计	被引频次：10[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.yic.ac.cn/handle/133337/27239
专题	中国科学院海岸带环境过程与生态修复重点实验室
通讯作者	Wu, Huifeng
作者单位	1.Chinese Acad Sci, CAS Key Lab Coastal Environm Proc & Ecol Remediat, Yantai Inst Coastal Zone Res YIC, Yantai 264003, Peoples R China 2.YICCAS, Shandong Key Lab Coastal Environm Proc, Yantai 264003, Peoples R China 3.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Fisheries Sci & Food Prod Proc, Qingdao 266237, Peoples R China 4.Chinese Acad Sci, Ctr Ocean Mega Sci, Qingdao 266071, Peoples R China 5.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Zhan, Junfei,Wang, Shuang,Li, Fei,et al. Global characterization of dose-dependent effects of cadmium in clam Ruditapes philippinarum[J]. ENVIRONMENTAL POLLUTION,2021,273:9.
APA	Zhan, Junfei,Wang, Shuang,Li, Fei,Ji, Chenglong,&Wu, Huifeng.(2021).Global characterization of dose-dependent effects of cadmium in clam Ruditapes philippinarum.ENVIRONMENTAL POLLUTION,273,9.
MLA	Zhan, Junfei,et al."Global characterization of dose-dependent effects of cadmium in clam Ruditapes philippinarum".ENVIRONMENTAL POLLUTION 273(2021):9.

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