海岸带环境工程技术研究与发展中心知识库

(1) 烟台大学药学院分子药理和药物评价教育部重点实验室新型制剂与生物技术药物研究山东省高校协同创新中心

Ginsenosides are an important kind of active ingredients in Panax genus and can be classified into four main types: the protopanaxadiols (PPD),protopanaxatriols (PPT),oleanolic acids (OA) and ocotillol type (OT). Recently,more than 620 kinds of these compounds have been isolated. Their chemical structures are similar but have quite different medicinal functions. It is of vital importance to develop simple and facile sample pretreatment methods and detection techniques to detect the content of ginsenosides in complex matrix. This review includes many kinds of sample pretreatment methods(such as liquid phase extraction,and solid phase extraction) and detection methods(such as high performance liquid chromatography,ultra-performance liquid chromatography,thin layer chromatography,and gas chromatography),etc.,summarizes the sensitivity and recovery of various methods,and reviews the advantages and disadvantages of each method and its research progress.

人参皂苷类化合物是参属类植物中的一类重要活性成分,主要包括原人参二醇、原人参三醇、齐墩果酸型、奥克梯隆型四类。最近,已发现的人参皂苷类化合物化学结构多达620余种,它们具有相似的化学结构,但药理活性具有明显差异。该类化合物所在基质复杂多样,选择简便高效的样品前处理方法及检测技术对于有效检测样品中人参皂苷含量至关重要。本文综述了测定各类样品中多种人参皂苷含量的样品前处理技术(溶剂提取、固相萃取等)及常用检测方法(高效液相色谱法、超高效液相色谱法、薄层色谱法、气相色谱法等),对各种方法的灵敏度及回收率等参数进行了总结,并评述了每种方法的优缺点及研究进展。