| Inactivation of the Phosphatase CheZ Alters Cell-Surface Properties of Azorhizobium caulinodans ORS571 and Symbiotic Association with Sesbania rostrata | |
| Liu, XL; Xie, ZH | |
| 发表期刊 | MOLECULAR PLANT-MICROBE INTERACTIONS
 |
| ISSN | 0894-0282 |
| 2019-11 | |
| 卷号 | 32期号:11页码:1547-1556 |
| 关键词 | genetics and gene regulation rhizobium-legume symbiosis |
| 研究领域 | Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology ; Plant Sciences |
| DOI | 10.1094/MPMI-05-19-0143-R |
| 产权排序 | [Liu, Xiaolin; Xie, Zhihong] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Biol & Bioresource Utilizat, Yantai, Peoples R China; [Liu, Xiaolin] Univ Chinese Acad Sci, Beijing, Peoples R China; [Liu, Xiaolin; Xie, Zhihong] Chinese Acad Sci, Ctr Ocean Mag Sci, Qingdao, Shandong, Peoples R China |
| 通讯作者 | Xie, Zhihong([email protected]) |
| 作者部门 | 海岸带生物学与生物资源保护实验室 |
| 英文摘要 | Azorhizobium caulinodans can form root and stem nodules with the host plant Sesbania rostrata. The role of the CheZ phosphatase in the A. caulinodans chemotaxis pathway was previously explored using the nonchemotactic cheZ mutant strain (AC601). This mutant displayed stronger attachment to the root surface, enhancing early colonization; however, this did not result in increased nodulation efficiency. In this study, we further investigated the role of CheZ in the interaction between strain ORS571 and the roots of its host plant. By tracking long-term colonization dynamic of cheZ mutant marked with LacZ, we found a decrease of colonization of the cheZ mutant during this process. Furthermore, the cheZ mutant could not spread on the root surface freely and was gradually outcompeted by the wild type in original colonization sites. Quantitative reverse-transcription PCR analyses showed that exp genes encoding exopolysaccharides synthesis, including oac3, were highly expressed in the cheZ mutant. Construction of a strain carrying a deletion of both cheZ and oac3 resulted in a mutant strain defective in the colonization process to the same extent as found with the oac3 single-mutant strain. This result suggested that the enhanced colonization of the cheZ mutant may be achieved through regulating the formation of exopolysaccharides. This shows the importance of the chemotactic proteins in the interaction between rhizobia and host plants, and expands our understanding of the symbiosis interaction between rhizobium and host plant. |
| 文章类型 | Article |
| 资助机构 | National Natural Science Foundation of ChinaNational Natural Science Foundation of China [31570063, 31870020] ; National Natural Science Foundation of China (NSFC-Shandong Joint Fund Key Project) [U1806206] ; Key Deployment Project of the Chinese Academy of Sciences [KFZD-SW-112] ; Agricultural Scientific and Technological Innovation Project of Shandong Academy of Agricultural Sciences [+CXGC2016B10] |
| 收录类别 | SCI |
| 语种 | 英语 |
| 关键词[WOS] | SIGNAL-TRANSDUCTION CASCADE ; NODULE DEVELOPMENT ; ROOT COLONIZATION ; BIOFILM FORMATION ; CHEMOTAXIS ; LIPOPOLYSACCHARIDE ; NODULATION ; SYSTEM ; EXOPOLYSACCHARIDE ; POLYSACCHARIDE |
| 研究领域[WOS] | Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology ; Plant Sciences |
| WOS记录号 | WOS:000494258900011 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.yic.ac.cn/handle/133337/24825 |
| 专题 | 海岸带生物学与生物资源利用重点实验室_海岸带生物学与生物资源保护实验室 |
| 作者单位 | 1.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Biol & Bioresource Utilizat, Yantai, Peoples R China; 2.Univ Chinese Acad Sci, Beijing, Peoples R China; 3.Chinese Acad Sci, Ctr Ocean Mag Sci, Qingdao, Shandong, Peoples R China |
| 推荐引用方式 GB/T 7714 | Liu, XL,Xie, ZH. Inactivation of the Phosphatase CheZ Alters Cell-Surface Properties of Azorhizobium caulinodans ORS571 and Symbiotic Association with Sesbania rostrata[J]. MOLECULAR PLANT-MICROBE INTERACTIONS,2019,32(11):1547-1556. |
| APA | Liu, XL,&Xie, ZH.(2019).Inactivation of the Phosphatase CheZ Alters Cell-Surface Properties of Azorhizobium caulinodans ORS571 and Symbiotic Association with Sesbania rostrata.MOLECULAR PLANT-MICROBE INTERACTIONS,32(11),1547-1556. |
| MLA | Liu, XL,et al."Inactivation of the Phosphatase CheZ Alters Cell-Surface Properties of Azorhizobium caulinodans ORS571 and Symbiotic Association with Sesbania rostrata".MOLECULAR PLANT-MICROBE INTERACTIONS 32.11(2019):1547-1556. |
| 条目包含的文件 | ||||||
| 文件名称/大小 | 文献类型 | 版本类型 | 开放类型 | 使用许可 | ||
| Inactivation of the （1870KB） | 期刊论文 | 出版稿 | 开放获取 | CC BY-NC-SA | 浏览 请求全文 | |
| 个性服务 |
| 推荐该条目 |
| 保存到收藏夹 |
| 查看访问统计 |
| 导出为Endnote文件 |
| 谷歌学术 |
| 谷歌学术中相似的文章 |
| [Liu, XL]的文章 |
| [Xie, ZH]的文章 |
| 百度学术 |
| 百度学术中相似的文章 |
| [Liu, XL]的文章 |
| [Xie, ZH]的文章 |
| 必应学术 |
| 必应学术中相似的文章 |
| [Liu, XL]的文章 |
| [Xie, ZH]的文章 |
| 相关权益政策 |
| 暂无数据 |
| 收藏/分享 |
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。
修改评论