| Two macrophage migration inhibitory factors (MIFs) from the clam Ruditapes philippinarum: Molecular characterization, localization and enzymatic activities | |
Wang, Dan; Yang, Dinglong ; Wang, Qing; Zhao, Ye; Li, Chenghua; Wei, Qianyu; Han, Yijing; Zhao, Jianmin
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| 发表期刊 | FISH & SHELLFISH IMMUNOLOGY
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| ISSN | 1050-4648 |
| 2018-07 | |
| 卷号 | 78页码:158-168 |
| 关键词 | Manila clam Macrophage migration inhibitory factor Immune response Enzymatic activity |
| 研究领域 | Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences |
| DOI | 10.1016/j.fsi.2018.04.026 |
| 产权排序 | [Wang, Dan; Li, Chenghua; Wei, Qianyu] Ningbo Univ, Ningbo 315211, Zhejiang, Peoples R China; [Yang, Dinglong; Wang, Qing; Han, Yijing; Zhao, Jianmin] Chinese Acad Sci, Yantai Inst Coastal Zone Res YIC, Muping Coastal Environm Res Stn, Yantai 264003, Peoples R China; [Zhao, Ye] Yantai Univ, Ocean Sch, Yantai 264005, Peoples R China; [Yang, Dinglong; Wang, Qing; Han, Yijing; Zhao, Jianmin] Chinese Acad Sci, Res & Dev Ctr Efficient Utilizat Coastal Bioresou, Yantai Inst Coastal Zone Res, Yantai 264003, Shandong, Peoples R China; [Han, Yijing] Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 作者部门 | 中国科学院牟平海岸带环境综合试验站 |
| 英文摘要 | Macrophage migration inhibitory factor (MIF) is an evolutionarily ancient cytokine-like factor and plays a critical role in both innate and adaptive immunity. In the present study, two MIFs (designed as RpMIF-1 and RpMIF-2, respectively) were identified and characterized from the clam Ruditapes philippinarum by rapid amplification of cDNA ends (RACE) approaches. The full-length cDNA of RpMIF-1 and RpMFI-2 consisted of 531 and 722 nucleotides, encoding a polypeptide of 113 and 114 amino acid residues, respectively. Multiple alignments and phylogenetic analysis revealed that both RpMIF-1 and RpMIF-2 belonged to the MIF family. The conserved catalytic-site Pro(2) for tautomerase activity was identified in the deduced amino acid sequences of RpMIFs. Both RpMIF-1 and RpMIF-2 transcripts were constitutively expressed in examined tissues of R. philippinarum with dominant expression in hepatopancreas, gills and hemocytes. Immunolocalization analysis showed that RpMIF-1 and RpMIF-2 proteins were expressed in examined tissues with the exception of adductor muscle and foot. After Vibrio anguillarum and Micrococcus luteus challenge, the mRNA expression of RpMIFs was significantly modulated in hemocytes, gills and hepatopancreas. Recombinant RpMIF-1 and RpMIF-2 proteins possessed significant tautomerase activity and oxidoreductase activity, indicating that these two proteins was perhaps involved in inflammatory responses. In summary, our results suggested that RpMIF-1 and RpMIF-2 played an important role in the innate immunity of R. philippinarum. |
| 文章类型 | Article |
| 资助机构 | Strategic Priority Research Program of the Chinese Academy of Sciences [XDA11020702] ; National Natural Science Foundation of China [41576122] ; Youth Innovation Promotion Association CAS [2016196] ; K.C. Wong Magna Fund at Ningbo University |
| 收录类别 | SCI |
| 语种 | 英语 |
| 关键词[WOS] | SCALLOP CHLAMYS-FARRERI ; INNATE IMMUNE-RESPONSES ; TOLL-LIKE RECEPTOR-4 ; MARINE-INVERTEBRATES ; REGULATORY ROLE ; DANIO-RERIO ; CYTOKINE ; GENE ; EXPRESSION ; INVOLVEMENT |
| 研究领域[WOS] | Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences |
| WOS记录号 | WOS:000437385000019 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.yic.ac.cn/handle/133337/24444 |
| 专题 | 支撑部门_中国科学院牟平海岸带环境综合试验站 海岸带生物学与生物资源利用重点实验室_海岸带生物学与生物资源保护实验室 海岸带生物学与生物资源利用重点实验室_海岸带生物资源高效利用研究与发展中心 |
| 作者单位 | 1.Ningbo Univ, Ningbo 315211, Zhejiang, Peoples R China; 2.Chinese Acad Sci, Yantai Inst Coastal Zone Res YIC, Muping Coastal Environm Res Stn, Yantai 264003, Peoples R China; 3.Yantai Univ, Ocean Sch, Yantai 264005, Peoples R China; 4.Chinese Acad Sci, Res & Dev Ctr Efficient Utilizat Coastal Bioresou, Yantai Inst Coastal Zone Res, Yantai 264003, Shandong, Peoples R China; 5.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Dan,Yang, Dinglong,Wang, Qing,et al. Two macrophage migration inhibitory factors (MIFs) from the clam Ruditapes philippinarum: Molecular characterization, localization and enzymatic activities[J]. FISH & SHELLFISH IMMUNOLOGY,2018,78:158-168. |
| APA | Wang, Dan.,Yang, Dinglong.,Wang, Qing.,Zhao, Ye.,Li, Chenghua.,...&Zhao, Jianmin.(2018).Two macrophage migration inhibitory factors (MIFs) from the clam Ruditapes philippinarum: Molecular characterization, localization and enzymatic activities.FISH & SHELLFISH IMMUNOLOGY,78,158-168. |
| MLA | Wang, Dan,et al."Two macrophage migration inhibitory factors (MIFs) from the clam Ruditapes philippinarum: Molecular characterization, localization and enzymatic activities".FISH & SHELLFISH IMMUNOLOGY 78(2018):158-168. |
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