水分条件对滨海芦苇湿地土壤微生物多样性的影响

doi:DOI: 10.11759//hykx20161018001

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	水分条件对滨海芦苇湿地土壤微生物多样性的影响
其他题名	Effects of water conditions on the diversity of soil microbial communities in the coastal reed wetlands
	张洪霞 ; 郑世玲 ; 魏文超; 王炳臣 ; 王欧美 ; 刘芳华
发表期刊	海洋科学
ISSN	1000-3096
	2017-05-15
卷号	41 期号:5 页码:144-152
关键词	water condition reed wetlands microbial community iron-reducing bacteria diversity 水分条件芦苇湿地微生物群落铁还原菌多样性
DOI	DOI: 10.11759//hykx20161018001
产权排序	(1) 中国科学院烟台海岸带研究所海岸带生物学与生物资源利用所重点实验室; (2) 中国科学院大学; (3) 滨州医学院
作者部门	海岸带生物学与生物资源保护实验室
英文摘要	River-sea water interactions in coastal wetlands dramatically affect soil redox potential and cause variations in nutrient elements. In this study, we analyzed the changes in physicochemical properties of soil, diversity in the microbial community, and the Fe(III) reduction activity of microorganisms in reed wetlands of the Yellow River Delta under different water conditions. The results showed that the salinity, pH value, and concentrations of organic carbon, total nitrogen, and Fe(II) in the soil were higher in the flooded reed wetlands as compared with the ammonium concentration. The genera Alishewanella, Cellulomonas, Sphingomonas and Pseudomonas were the dominated bacteria in flooded reed wetlands, whereas Methanosarcina and Methanobacterium were the dominant archaea. Further, the dominated bacteria and archaea in the non-flooded reed wetlands were Cellulomonas, Acidovorax, Geobacter, Thiobacillus, and Methanosarcina, respectively. A shift in the iron-reducing bacterial community structure was also observed in the enrichment samples from flooded and non-flooded reed wetlands. Paracoccus, Geobacter, Alishewanella, and Thiomonas were the dominant iron-reducing bacteria in the flooded reed wetlands, and the dominant genera of iron-reducing bacteria in the non-flooded reed wetlands were Geobacter and Azospira. The diversity of soil bacteria, archaea, and iron-reducing bacteria was higher in the flooded reed wetlands. The Fe(III) reduction activity of iron-reducing bacteria in the reed wetlands was lessened to a certain extent by perennial flooded conditions.
中文摘要	滨海湿地因受河流和海水的交互作用,其土壤氧化还原电位及营养元素等条件复杂且处于不断变化之中,为了阐明此种环境下不同水分条件对滨海芦苇湿地土壤微生物多样性的影响,尤其是对铁还原菌群落结构的影响,作者以黄河三角洲滨海湿地为对象,研究了淹水和非淹水芦苇湿地表层(0~30 cm)土壤理化特性、微生物多样性(采用T-RFLP技术)和培养条件下湿地土壤微生物的Fe(III)还原能力的变化。结果表明,淹水芦苇湿地土壤有机碳、全氮、盐度、Fe(II)含量和pH值显著(P<0.05)高于非淹水芦苇湿地土壤,而淹水芦苇湿地土壤铵态氮含量显著(P<0.05)低于非淹水芦苇湿地。淹水芦苇湿地土壤主要优势细菌和古菌分别为铬还原菌属(Alishewanella)、纤维单胞菌属(Cellulomonas)、鞘脂单胞菌属(Sphingomonas)、假单胞菌属(Pseudomonas)和甲烷杆菌属(Methanobacterium)、甲烷八叠球菌属(Methanosarcina);而纤维单胞菌属、食酸菌属(Acidovorax)、地杆菌属(Geobacter)、硫杆菌属(Thiobacillus)和甲烷八叠球菌属分别为非淹水芦苇湿地土壤主要优势细菌和古菌。淹水芦苇湿地土壤主要优势铁还原菌为副球菌属(Paracoccus)、地杆菌属、铬还原菌属和硫单胞菌属(Thiomonas),而非淹水芦苇湿地土壤为地杆菌属和固氮螺菌属(Azospira)。说明淹水能增加芦苇湿地土壤细菌、古菌和铁还原菌的多样性。培养条件下,淹水芦苇湿地土壤铁还原菌的还原Fe(III)能力在培养后期(25d后)低于非淹水芦苇湿地,说明淹水条件在一定程度上抑制了芦苇湿地土壤铁还原菌的铁还原能力。
文章类型	Article
收录类别	CSCD
语种	中文
关键词[WOS]	Microbiology
研究领域[WOS]	Microbiology ; 微生物学
CSCD记录号	CSCD:6058840
引用统计
文献类型	期刊论文
条目标识符	http://ir.yic.ac.cn/handle/133337/24295
专题	海岸带生物学与生物资源利用重点实验室_海岸带生物学与生物资源保护实验室
作者单位	1.中国科学院烟台海岸带研究所海岸带生物学与生物资源利用所重点实验室; 2.中国科学院大学; 3.滨州医学院
推荐引用方式 GB/T 7714	张洪霞,郑世玲,魏文超,等. 水分条件对滨海芦苇湿地土壤微生物多样性的影响[J]. 海洋科学,2017,41(5):144-152.
APA	张洪霞,郑世玲,魏文超,王炳臣,王欧美,&刘芳华.(2017).水分条件对滨海芦苇湿地土壤微生物多样性的影响.海洋科学,41(5),144-152.
MLA	张洪霞,et al."水分条件对滨海芦苇湿地土壤微生物多样性的影响".海洋科学 41.5(2017):144-152.