Agrobacterium salinitolerans sp nov., a saline-alkaline-tolerant bacterium isolated from root nodule of Sesbania cannabina

doi:10.1099/ijsem.0.001885

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	Agrobacterium salinitolerans sp nov., a saline-alkaline-tolerant bacterium isolated from root nodule of Sesbania cannabina
	Yan, Jun 1; Li, Yan2 ; Yan, Hui 3,4,5; Chen, Wen Feng 3,4,5; Zhang, Xiaoxia 6; Wang, En Tao 7; Han, Xiao Zeng 1; Xie, Zhi Hong2
发表期刊	INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY
ISSN	1466-5026
	2017-06-01
卷号	67 期号:6 页码:1906-1911
关键词	Agrobacterium Salinitolerans Sp Nov. Sesbania Cannabina Polyphasic Taxonomy Symbiosis
研究领域	Microbiology
DOI	10.1099/ijsem.0.001885
产权排序	[Yan, Jun; Han, Xiao Zeng] Chinese Acad Sci, Northeast Inst Geog & Agroecol, Key Lab Mollisols Agroecol, Harbin 150081, Peoples R China; [Li, Yan; Xie, Zhi Hong] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Biol & Utilizat, Yantai 264003, Peoples R China; [Yan, Hui; Chen, Wen Feng] State Key Lab Agrobiotechnol, Beijing 100193, Peoples R China; [Yan, Hui; Chen, Wen Feng] China Agr Univ, Rhizobium Res Ctr, Beijing 100193, Peoples R China; [Yan, Hui; Chen, Wen Feng] China Agr Univ, Coll Biol Sci, Beijing 100193, Peoples R China; [Zhang, Xiaoxia] Chinese Acad Agr Sci, Inst Agr Resources & Reg Planning, Minist Agr, Key Lab Microbial Resources Collect & Preservat, Beijing 100081, Peoples R China; [Wang, En Tao] Inst Politecn Nacl, Escuela Nacl Ciencias Biol, Dept Microbiol, Mexico City 11340, DF, Mexico
作者部门	海岸带生物学与生物资源保护实验室
英文摘要	Two Gram-staining-negative, aerobic bacteria (YIC 5082(T) and YIC4104) isolated from root nodules of Sesbania cannabina grown in a high-salt and alkaline environment were identified as a group in the genus Agrobacterium because they shared 100 and 99.7% sequence similarities of 16S rRNA and recA+ atpD genes, respectively. These two strains showed 99.2/100% and 93.9/95.4% 16S rRNA and recA+ atpD gene sequence similarities to Agrobacterium radiobacter LMG140 T and Agrobacterium. pusense NRCPB10 T, respectively. The average nucleotide identities (ANI) of genome sequences were 89.95% or lower between YIC 5082 T and the species of the genus Agrobacterium examined. Moreover, these two test strains formed a unique nifH lineage deeply separated from other rhizobia. Although the nodC gene was not detected in YIC 5082(T) and YIC4104, they could form effective root nodules on S. cannabina plants. The main cellular fatty acids in YIC 5082(T) were summed feature 8 (C-18 : 1 omega 7c/C-18 : 1 omega 6c), C-19 : 0 omega 8c, summed feature 2 (C-12 : 0 aldehyde/unknown equivalent chain length 10.9525) and C16 : 0. The DNA G+C content of YIC 5082 T was 59.3 mol%. The failure to utilize D-sorbitol as a carbon source distinguished YIC 5082(T) from the type strains of related species. YIC 5082 T could grow in presence of 5.0% (w/v) NaCl and at a pH of up to 10.0. Based on results regarding the genetic and phenotypic properties of YIC 5082(T) and YIC4104 the name Agrobacterium salinitolerans sp. nov. is proposed and YIC 5082 T (= HAMBI 3646 T = LMG 29287(T)) is designed as the type strain.
文章类型	Article
资助机构	National Natural Science Foundation of China(41671299 ; National Key Research and Development Program of China(2016YFD0200300) ; national research program of China(2016YFD0200300) ; Instituto Politecnico Nacional, Mexico(SIP 20150597 ; 41371296 ; 20140124) ; 31370108 ; 31570063)
收录类别	SCI
语种	英语
关键词[WOS]	CLOSE PHYLOGENETIC RELATIONSHIP ; GENETIC DIVERSITY ; RHIZOBIUM-GALEGAE ; SEQUENCES ; CLASSIFICATION ; IDENTIFICATION ; MESORHIZOBIUM ; SINORHIZOBIUM ; SYMBIONTS ; TAXONOMY
研究领域[WOS]	Microbiology
WOS记录号	WOS:000405503800046
引用统计	被引频次：20[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.yic.ac.cn/handle/133337/22516
专题	海岸带生物学与生物资源利用重点实验室_海岸带生物学与生物资源保护实验室
作者单位	1.Chinese Acad Sci, Northeast Inst Geog & Agroecol, Key Lab Mollisols Agroecol, Harbin 150081, Peoples R China 2.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Biol & Utilizat, Yantai 264003, Peoples R China 3.State Key Lab Agrobiotechnol, Beijing 100193, Peoples R China 4.China Agr Univ, Rhizobium Res Ctr, Beijing 100193, Peoples R China 5.China Agr Univ, Coll Biol Sci, Beijing 100193, Peoples R China 6.Chinese Acad Agr Sci, Inst Agr Resources & Reg Planning, Minist Agr, Key Lab Microbial Resources Collect & Preservat, Beijing 100081, Peoples R China 7.Inst Politecn Nacl, Escuela Nacl Ciencias Biol, Dept Microbiol, Mexico City 11340, DF, Mexico
推荐引用方式 GB/T 7714	Yan, Jun,Li, Yan,Yan, Hui,et al. Agrobacterium salinitolerans sp nov., a saline-alkaline-tolerant bacterium isolated from root nodule of Sesbania cannabina[J]. INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY,2017,67(6):1906-1911.
APA	Yan, Jun.,Li, Yan.,Yan, Hui.,Chen, Wen Feng.,Zhang, Xiaoxia.,...&Xie, Zhi Hong.(2017).Agrobacterium salinitolerans sp nov., a saline-alkaline-tolerant bacterium isolated from root nodule of Sesbania cannabina.INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY,67(6),1906-1911.
MLA	Yan, Jun,et al."Agrobacterium salinitolerans sp nov., a saline-alkaline-tolerant bacterium isolated from root nodule of Sesbania cannabina".INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY 67.6(2017):1906-1911.