A Global View of Gene Expression of Aspergillus nidulans on Responding to the Deficiency in Soluble Potassium

doi:10.1007/s00284-015-0963-y

	A Global View of Gene Expression of Aspergillus nidulans on Responding to the Deficiency in Soluble Potassium
	Xiao, LL; Sun, QBA ; Lian, B ; Lian, B (reprint author), Nanjing Normal Univ, Coll Life Sci, Jiangsu Engn & Technol Res Ctr Microbiol, Jiangsu Key Lab Microbes & Funct Genom, Nanjing 210023, Jiangsu, Peoples R China. [email protected]; [email protected]; [email protected]
发表期刊	CURRENT MICROBIOLOGY
ISSN	0343-8651
	2016-04-01
卷号	72 期号:4 页码:410-419
DOI	10.1007/s00284-015-0963-y
产权排序	[Xiao, Leilei; Sun, Qibiao; Lian, Bin] Nanjing Normal Univ, Coll Life Sci, Jiangsu Engn & Technol Res Ctr Microbiol, Jiangsu Key Lab Microbes & Funct Genom, Nanjing 210023, Jiangsu, Peoples R China; [Xiao, Leilei] Chinese Acad Sci, Inst Coastal Zone Res, Key Lab Coastal Biol & Utilizat, Yantai 264003, Shandong, Peoples R China
作者部门	海岸带生物学与生物资源利用所重点实验室
英文摘要	Many researchers have suggested that microbes can accelerate the weathering of silicate minerals. However, many genes and metabolic pathways related to microorganisms obtaining potassium (K) from silicate remain undiscovered. It is feasible to detect the gene expression within the scope of the whole genome through high-throughput sequencing. Surprisingly, only a few reports have shown fungal weathering of silicate using this technology. This study explored differences in gene expression of Aspergillus nidulans, which was cultured with different K sources, KCl, and K-feldspar. A. nidulans RNA was extracted by the construction of a cDNA library. Identification of K-acquisition-related genes with GO and KEGG pathway analysis revealed that primarily differentially expressed genes were related to the biosynthesis of amino acids. When these genes were grouped in accordance with the physiological functions, the genes involved in the synthesis of protease, ribosome, and mitochondria, trans-membrane transport, and oxidative phosphorylation were significantly different. Moreover, 20 genes selected were further tested using RT-qPCR. One half (10 genes) exhibited differential expression, which was consistent with the results of RNA-seq. Combining the results of RNA-seq and RT-qPCR, we summarised a possible way to obtain mineral K in A. nidulans as well. The differentially expressed genes and their associated metabolic pathways revealed will improve the understanding of the molecular mechanisms of microbial weathering of silicate minerals.
文章类型	Article
资助机构	National Natural Science Foundation of China(41173091 ; 41373078)
收录类别	SCI
语种	英语
关键词[WOS]	CARBONIC-ANHYDRASE ; BEARING MINERALS ; ARABIDOPSIS ; STRESS ; ECTOMYCORRHIZAL ; TRANSCRIPTOME ; SYSTEM ; EUKARYOTES ; PATHWAYS ; BIOTITE
研究领域[WOS]	Microbiology
WOS记录号	WOS:000371250500008
引用统计	被引频次：3[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.yic.ac.cn/handle/133337/10841
专题	中国科学院烟台海岸带研究所知识产出海岸带生物学与生物资源利用重点实验室_海岸带生物学与生物资源保护实验室
通讯作者	Lian, B (reprint author), Nanjing Normal Univ, Coll Life Sci, Jiangsu Engn & Technol Res Ctr Microbiol, Jiangsu Key Lab Microbes & Funct Genom, Nanjing 210023, Jiangsu, Peoples R China. [email protected]; [email protected]; [email protected]
作者单位	1.Nanjing Normal Univ, Coll Life Sci, Jiangsu Engn & Technol Res Ctr Microbiol, Jiangsu Key Lab Microbes & Funct Genom 2.Chinese Acad Sci, Inst Coastal Zone Res, Key Lab Coastal Biol & Utilizat
推荐引用方式 GB/T 7714	Xiao, LL,Sun, QBA,Lian, B,et al. A Global View of Gene Expression of Aspergillus nidulans on Responding to the Deficiency in Soluble Potassium[J]. CURRENT MICROBIOLOGY,2016,72(4):410-419.
APA	Xiao, LL,Sun, QBA,Lian, B,Lian, B ,[email protected],[email protected].(2016).A Global View of Gene Expression of Aspergillus nidulans on Responding to the Deficiency in Soluble Potassium.CURRENT MICROBIOLOGY,72(4),410-419.
MLA	Xiao, LL,et al."A Global View of Gene Expression of Aspergillus nidulans on Responding to the Deficiency in Soluble Potassium".CURRENT MICROBIOLOGY 72.4(2016):410-419.

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