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小清河口邻近海域渔业资源结构及三种常见经济种遗传多样性特征 学位论文
工程硕士, 中国科学院烟台海岸带研究所: 中国科学院烟台海岸带研究所, 2022
作者:  刘英
Adobe PDF(3608Kb)  |  收藏  |  浏览/下载：808/0  |  提交时间：2022/06/27
小清河口邻近海域  渔业资源  群落结构  经济种  遗传多样性  Xiaoqing River Estuary and its adjacent waters  Fishery resources  Community structure  Economic species  Genetic diversity  
2018年烟台四十里湾及其邻近海域网采浮游植物群落结构的时空变化 期刊论文
海洋与湖沼, 2020, 卷号: 51, 期号: 5, 页码: 1151-1161
作者:  张文静;  董志军;  孙西艳;  张晨;  刘永亮;  侯朝伟;  赵建民
Adobe PDF(2077Kb)  |  收藏  |  浏览/下载：316/27  |  提交时间：2021/06/16
Sishili Bay and its adjacent water  phytoplankton community  spatial and temporal distribution  environmental factors  四十里湾及其邻近海域  浮游植物群落结构  时空变化  环境因子  
入侵植物互花米草的资源化利用研究进展 期刊论文
中国生态农业学报(中英文), 2019, 卷号: 27, 期号: 12, 页码: 1870-1879
作者:  谢宝华, 路峰, 韩广轩
收藏  |  浏览/下载：453/0  |  提交时间：2020/01/09
入侵植物  互花米草  资源化利用  秸秆资源  药用价值  耐盐基因  Invasive plant  Spartina alterniflora  Resource utilization  Straw resource  Medicinal value  Salt tolerant gene  In China, Spartina alterniflora, an invasive alien plant species, covers an area of approximately 54 600 hectares. The total dry matter of the aboveground part of S. alterniflora is 7.5*10~5~1.15*10~6 tons per year. The control and utilization of S. alterniflora have received increasing attention. A combined utilization and control approach could produce simultaneous economic and ecological benefits. Studies of the utilization of S. alterniflora in China tended to focus on the direct utilization of straw, including its use as fuel, feed, and raw material. These methods of utilization were generally low-value. Possible high-value utilization includes the medicinal value of S. alterniflora and its salt-tolerant genes. In all the studies, the largest proportion (43%) of straw was utilized as fuel, whereas the smallest proportion (approximately 8%) was utilized for other purposes, such as fertilizer and for its salt-tolerant genes. Previous studies have focused on utilization technologies, but they have not looked at S. alterniflora collection technology and market promotion. In the future, attention should be paid to every link of the industry chain of S. alterniflora utilization, and cooperation between research institutions and enterprises is essential for the synchronous development of industry, education, and research.  
食用蔬菜能吸收和积累微塑料 期刊论文
科学通报, 2019, 卷号: 64, 期号: 9, 页码: 928-934
作者:  李连祯;  周倩;  尹娜;  涂晨;  骆永明
收藏  |  浏览/下载：536/0  |  提交时间：2020/06/17
微塑料  生菜  聚苯乙烯微球  吸收  积累  健康风险  microplastics  lettuce  polystyrene microbeads  uptake  accumulation  human health risk  Microplastic (MP, 100 nm-5 mm) may present an attributable risk to ecosystem and human health, and its pollution has become a global environmental concern. Despite a wealth of information on the accumulation of MPs in aquatic species, there is no information on the uptake and accumulation of MPs by higher plants. Terrestrial edible plants are directly exposed to MPs when agricultural soil was applied with organic manure, sewage sludge as fertilizer or plastic mulching. In this paper, the uptake of two sizes of polystyrene (PS) microbeads (0.2 and 1.0 mum) and then their distribution and migration in an edible plant lettuce were firstly investigated based on laboratory experiments. We used fluorescent markers to track PS microbeads in plant tissues and found fluorescence to be a sensitive and reliable detection method. Sections from untreated control lettuce showed no autofluorescence. When roots were treated with fluorescently labeled PS microbeads, the microbeads could be identified by its fluorescence. Our main study investigated the uptake of 0.2 mum beads, as few luminescence signals were observed in lettuce roots for 1.0 mum beads in our experiment. We observed that 0.2 mum fluorescent microbeads were extracellularly trapped in the root cap mucilage (which is a highly hydrated polysaccharide) and a dark green tip (which was typical of lettuce roots exposed to label PS beads) was usually visible to the naked eye. Confocal images revealed that the PS luminescence signals were mainly located in the vascular system and on the cell walls of the cortex tissue of the roots, indicated that the beads passed through the intercellular space via the apoplastic transport system. Once inside the central cylinder, the 0.2 mum PS beads were transferred from the roots to the stems and leaves via the vascular system following the transpiration stream. We also observed that the PS beads adhered to one another and self-assembled systematically into grape-like and (chain) string-like clusters in the intercellular space of the root and stem vascular tissue of lettuce plant. In contrast to the root and stem, PS beads were dispersed in the leaf tissue. Here, for the first time we provide evidence of the adherence, uptake, accumulation, and translocation of submicrometer MPs within an edible plant. Our findings highlight the previously underappreciated human exposure pathway to MPs through the consumption of contaminated crops and emphasize the need for new management strategies to control the release of MPs waste products into the terrestrial environment. Ultimately, the potential impacts of low range sized MPs on food safety of crop plants and human health need to be urgently considered.  
养殖水体三种大型爆发性海洋藻类孢子/配子实时荧光定量PCR检测方法的建立与应用初探 期刊论文
中国海洋大学学报(自然科学版), 2018, 卷号: 48, 期号: 08, 页码: 68-76
作者:  缪栋;  苏蕾;  张倩倩;  包卫洋;  龚骏
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藻类爆发  石莼  萱藻  特异性引物  孢子  配子  ITS rDNA  实时荧光定量PCR  
Development and evaluation of specific PCR primers targeting the ribosomal DNA-internal transcribed spacer (ITS) region of peritrich ciliates in environmental samples 期刊论文
JOURNAL OF OCEANOLOGY AND LIMNOLOGY, 2018, 卷号: 36, 期号: 3, 页码: 818-826
作者:  Su Lei;  Zhang Qianqian;  Gong Jun
Adobe PDF(639Kb)  |  收藏  |  浏览/下载：306/67  |  提交时间：2020/07/08
Ciliophora  Peritrichia  clone library  internal transcribed spacer (ITS)  rDNA  specific PCR primers  
ITS克隆测序揭示中国沿海表层水中真核浮游生物多样性 期刊论文
海洋科学进展, 2018, 卷号: 36, 期号: 01, 页码: 108-116
作者:  于淑贤;  李文军;  李楠;  李富超;  王寅初;  李佳霖;  秦松
Adobe PDF(6597Kb)  |  收藏  |  浏览/下载：339/57  |  提交时间：2020/07/08
真核浮游生物  中国沿海  ITS  生物多样性  
海草床底栖原生生物及黄渤海沉积物中纤毛虫的分子生态学研究 学位论文
, 北京: 中国科学院大学, 2017
作者:  苏蕾
Adobe PDF(7589Kb)  |  收藏  |  浏览/下载：466/2  |  提交时间：2017/06/12
微型真核生物  顶复门  纤毛虫  缘毛类纤毛虫  高通量测序  Its  特异性引物  分子多样性  群落结构  生物地理学  
绿潮暴发期间我国青岛漂浮铜藻的分子鉴定 期刊论文
生物学杂志, 2016, 卷号: 33, 期号: 1, 页码: 39-42
作者:  陈军;  王寅初;  余秋瑢;  毕燕会;  何培民;  刘正一;  秦松;  秦松,中国科学院烟台海岸带研究所,E-mail：[email protected]
Adobe PDF(1840Kb)  |  收藏  |  浏览/下载：534/130  |  提交时间：2016/12/09
漂浮铜藻  5.8s r Dna-its  分子鉴定  青岛  
Molecular diversity of Enteromorpha from the coast of Yantai: a dual-marker assessment 期刊论文
CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY, 2013, 卷号: 31, 期号: 6, 页码: 1210-1215
作者:  Liu Haiyan;  Liu Zhengyi;  Wang Yinchu;  Zhao Yushan;  Qin Song
收藏  |  浏览/下载：257/0  |  提交时间：2016/04/12
Enteromorpha  Internal Transcribed Spacer (Its)  5srdna Non-transcribed Spacer (Nts)  Molecular Diversity