Reference Gene Selection for qPCR Normalization of Kosteletzkya virginica under Salt Stress

doi:10.1155/2015/823806

	Reference Gene Selection for qPCR Normalization of Kosteletzkya virginica under Salt Stress
	Tang, XL; Wang, HY; Shao, CY; Shao, HB; Shao, HB (reprint author), Chinese Acad Sci, Yantai Inst Coastal Zone Res YIC, Key Lab Coastal Biol & Bioresources Utilizat, Yantai 264003, Peoples R China. [email protected]
发表期刊	BIOMED RESEARCH INTERNATIONAL
ISSN	2314-6133
	2015
DOI	10.1155/2015/823806
产权排序	[Tang, Xiaoli; Wang, Hongyan; Shao, Hongbo] Chinese Acad Sci, Yantai Inst Coastal Zone Res YIC, Key Lab Coastal Biol & Bioresources Utilizat, Yantai 264003, Peoples R China; [Tang, Xiaoli; Wang, Hongyan] Univ Chinese Acad Sci, Beijing 100039, Peoples R China; [Wang, Hongyan] China Agr Univ, Yantai Acad, Yantai 264670, Peoples R China; [Shao, Chuyang] Shandong Agr Univ, Coll Life Sci, Tai An 271018, Shandong, Peoples R China; [Shao, Hongbo] Jiangsu Acad Agr Sci, Inst Agrobiotechnol, Nanjing 210014, Jiangsu, Peoples R China
作者部门	海岸带生物学与生物资源利用所重点实验室
英文摘要	Kosteletzkya virginica (L.) is a newly introduced perennial halophytic plant. Presently, reverse transcription quantitative real-time PCR (qPCR) is regarded as the best choice for analyzing gene expression and its accuracy mainly depends on the reference genes which are used for gene expression normalization. In this study, we employed qPCR to select the most stable reference gene in K. virginica which showed stable expression profiles under our experimental conditions. The candidate reference genes were 18S ribosomal RNA (18SrRNA), beta-actin (ACT), alpha-tubulin (TUA), and elongation factor (EF). We tracked the gene expression profiles of the candidate genes and analyzed their stabilities through BestKeeper, geNorm, and NormFinder software programs. The results of the three programs were identical and 18SrRNA was assessed to be the most stable reference gene in this study. However, TUA was identified to be the most unstable. Our study proved again that the traditional reference genes indeed displayed a certain degree of variations under given experimental conditions. Importantly, our research also provides guidance for selecting most suitable reference genes and lays the foundation for further studies in K. virginica.; Kosteletzkya virginica (L.) is a newly introduced perennial halophytic plant. Presently, reverse transcription quantitative real-time PCR (qPCR) is regarded as the best choice for analyzing gene expression and its accuracy mainly depends on the reference genes which are used for gene expression normalization. In this study, we employed qPCR to select the most stable reference gene in K. virginica which showed stable expression profiles under our experimental conditions. The candidate reference genes were 18S ribosomal RNA (18SrRNA), beta-actin (ACT), alpha-tubulin (TUA), and elongation factor (EF). We tracked the gene expression profiles of the candidate genes and analyzed their stabilities through BestKeeper, geNorm, and NormFinder software programs. The results of the three programs were identical and 18SrRNA was assessed to be the most stable reference gene in this study. However, TUA was identified to be the most unstable. Our study proved again that the traditional reference genes indeed displayed a certain degree of variations under given experimental conditions. Importantly, our research also provides guidance for selecting most suitable reference genes and lays the foundation for further studies in K. virginica.
文章类型	Article
资助机构	National Natural Science Foundation of China(41171216) ; National Basic Research Program of China(2013CB430403) ; Jiangsu Autonomous Innovation of Agricultural Science Technology(CX(15)1005) ; Yantai Double-Hundred Talent Plan(XY-003-02) ; 135 Development Plan of YIC-CAS ; Science & Technology Development Plan of Shandong Province(010GSF10208)
收录类别	SCI
语种	英语
关键词[WOS]	REAL-TIME PCR ; QUANTITATIVE RT-PCR ; HOUSEKEEPING GENES ; INTERNAL CONTROL ; ABIOTIC STRESS ; EXPRESSION ; VALIDATION ; IDENTIFICATION ; SALINITY ; ARABIDOPSIS
研究领域[WOS]	Biotechnology & Applied Microbiology ; Research & Experimental Medicine
WOS记录号	WOS:000364685200001
引用统计	被引频次：10[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.yic.ac.cn/handle/133337/9536
专题	中国科学院海岸带环境过程与生态修复重点实验室
通讯作者	Shao, HB (reprint author), Chinese Acad Sci, Yantai Inst Coastal Zone Res YIC, Key Lab Coastal Biol & Bioresources Utilizat, Yantai 264003, Peoples R China. [email protected]
推荐引用方式 GB/T 7714	Tang, XL,Wang, HY,Shao, CY,et al. Reference Gene Selection for qPCR Normalization of Kosteletzkya virginica under Salt Stress[J]. BIOMED RESEARCH INTERNATIONAL,2015.
APA	Tang, XL,Wang, HY,Shao, CY,Shao, HB,&Shao, HB .(2015).Reference Gene Selection for qPCR Normalization of Kosteletzkya virginica under Salt Stress.BIOMED RESEARCH INTERNATIONAL.
MLA	Tang, XL,et al."Reference Gene Selection for qPCR Normalization of Kosteletzkya virginica under Salt Stress".BIOMED RESEARCH INTERNATIONAL (2015).

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