| Comparative Proteomic Analysis of Brassica napus in Response to Drought Stress | |
| Koh, Jin1; Chen, Gang2,3; Yoo, Mi-Jeong2; Zhu, Ning2; Dufresne, Daniel4; Erickson, John E.5; Shao, Hongbo7; Chen, Sixue1,2,6 | |
| 发表期刊 | JOURNAL OF PROTEOME RESEARCH
 |
| ISSN | 1535-3893 |
| 2015-08-01 | |
| 卷号 | 14期号:8页码:3068-3081 |
| 关键词 | Drought Stress Brassica Napus Proteomics Itraq Ptms Transcription |
| DOI | 10.1021/pr501323d |
| 英文摘要 | Drought is one of the most widespread stresses leading to retardation of plant growth and development. We examined proteome changes of an important oil seed crop, canola (Brassica napus L.), under drought stress over a 14-day period. Using iTRAQ LC-MS/MS, we identified 1976 proteins expressed during drought stress. Among them, 417 proteins showed significant changes in abundance, and 136, 244, 286, and 213 proteins were differentially expressed in the third, seventh, 10th, and 14th day of stress, respectively. Functional analysis indicated that the number of proteins associated with metabolism, protein folding and degradation, and signaling decreased, while those related to energy (photosynthesis), protein synthesis, and stress and defense increased in response to drought stress. The seventh and 10th-day profiles were similar to each other but with more post-translational modifications (PTMs) at day 10. Interestingly, 181 proteins underwent PTMs; 49 of them were differentially changed in drought-stressed plants, and 33 were observed at the 10th day. Comparison of protein expression changes with those of gene transcription showed a positive correlation in B. napus, although different patterns between transcripts and proteins were observed at each time point. Under drought stress, most protein abundance changes may be attributed to gene transcription, and PTMs clearly contribute to protein diversity and functions. |
| 文章类型 | Article |
| 收录类别 | SCI |
| 语种 | 英语 |
| 关键词[WOS] | WATER-DEFICIT CONDITIONS ; BARLEY LEAF PROTEOME ; TRITICUM-AESTIVUM L. ; ARABIDOPSIS-THALIANA ; ABSCISIC-ACID ; ABIOTIC STRESS ; HEAT-STRESS ; PHOSPHOPROTEOME ANALYSIS ; QUANTITATIVE PROTEOMICS ; SIGNAL-TRANSDUCTION |
| 研究领域[WOS] | Biochemistry & Molecular Biology |
| WOS记录号 | WOS:000359394000006 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.yic.ac.cn/handle/133337/9287 |
| 专题 | 海岸带生物学与生物资源利用重点实验室_海岸带生物学与生物资源保护实验室 |
| 作者单位 | 1.Univ Florida, Genet Inst, Prote & Mass Spectrometry, Interdisciplinary Ctr Biotechnol Res, Gainesville, FL 32610 USA 2.Univ Florida, Genet Inst, Dept Biol, Gainesville, FL 32610 USA 3.Yangzhou Univ, Yangzhou 225009, Jiangsu, Peoples R China 4.Palm Beach Cent High Sch, Wellington, FL 33411 USA 5.Univ Florida, Dept Agron, Gainesville, FL 32611 USA 6.Univ Florida, Plant Mol & Cellular Biol, Gainesville, FL 32611 USA 7.Chinese Acad Sci, Yantai Inst Costal Zone Res, Yantai 264003, Shandong, Peoples R China |
| 推荐引用方式 GB/T 7714 | Koh, Jin,Chen, Gang,Yoo, Mi-Jeong,et al. Comparative Proteomic Analysis of Brassica napus in Response to Drought Stress[J]. JOURNAL OF PROTEOME RESEARCH,2015,14(8):3068-3081. |
| APA | Koh, Jin.,Chen, Gang.,Yoo, Mi-Jeong.,Zhu, Ning.,Dufresne, Daniel.,...&Chen, Sixue.(2015).Comparative Proteomic Analysis of Brassica napus in Response to Drought Stress.JOURNAL OF PROTEOME RESEARCH,14(8),3068-3081. |
| MLA | Koh, Jin,et al."Comparative Proteomic Analysis of Brassica napus in Response to Drought Stress".JOURNAL OF PROTEOME RESEARCH 14.8(2015):3068-3081. |
| 条目包含的文件 | 条目无相关文件。 | |||||
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。
修改评论