| ZnO Nanoparticles Treatment Induces Apoptosis by Increasing Intracellular ROS Levels in LTEP-a-2 Cells |
| Wang, CX; Hu, XK ; Gao, Y; Ji, YL; Hu, XK (reprint author), Chinese Acad Sci, Yantal Inst Coastal Zone Res, Key Lab Coastal Biol & Bioresource Utilizat, 17 Chunhui Rd, Yantai 264003, Peoples R China. [email protected]
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| 发表期刊 | BIOMED RESEARCH INTERNATIONAL
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| ISSN | 2314-6133
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| 2015
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| DOI | 10.1155/2015/423287
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| 产权排序 | [Wang, Caixia; Hu, Xiaoke] Chinese Acad Sci, Yantal Inst Coastal Zone Res, Key Lab Coastal Biol & Bioresource Utilizat, Yantai 264003, Peoples R China; [Wang, Caixia] Univ Chinese Acad Sci, Beijing 100049, Peoples R China; [Gao, Yan] Natl Oceanog Ctr, Qingdao 266071, Peoples R China; [Ji, Yinglu] Ocean Univ China, Coll Marine Life Sci, Qingdao 266003, Peoples R China |
| 作者部门 | 海岸带生物学与生物资源利用所重点实验室
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| 英文摘要 | Owing to the wide use of novel nanoparticles (NPs) such as zinc oxide (ZnO) in all aspects of life, toxicological research on ZnO NPs is receiving increasing attention in these days. In this study, the toxicity of ZnO NPs in a human pulmonary adcnocarcinoma cell line 1_,TEP-a-2 was tested in vitro. Log-phase cells were exposed to different levels of ZnO NPs for hours, followed by colorimetric cell viability assay using tetrazoliwn salt and cell survival rate assay using trypan blue dye. Cell morphological changes were observed by Giemsa staining and light microscopy. Apoptosis was detected by using fluorescence microscopy and caspase-3 activity assay. Both intracellular reactive oxygen species (ROS) and reduced glutathione (GSH) were examined by a microplate-reader method. Results showed that ZnO NPs (0.011.1g/mL) significantly inhibited proliferation (P < 0.05) and induced substantial apoptosis in 1,TEP-a-2 cells after 4 h of exposure. The intracellular ROS level rose up to 30-40% corresponding to significant depletion (approximately 70-80%) in GSH content in LTEP-a-2 cells (P < 0.05), suggesting that ZnO NPs induced apoptosis mainly through increased ROS production. This study elucidates the toxicological mechanism of ZnO NPs in human pulmonary adenocarcinoma cells and provides reference data for application of nanomaterials in the environment.; Owing to the wide use of novel nanoparticles (NPs) such as zinc oxide (ZnO) in all aspects of life, toxicological research on ZnO NPs is receiving increasing attention in these days. In this study, the toxicity of ZnO NPs in a human pulmonary adcnocarcinoma cell line 1_,TEP-a-2 was tested in vitro. Log-phase cells were exposed to different levels of ZnO NPs for hours, followed by colorimetric cell viability assay using tetrazoliwn salt and cell survival rate assay using trypan blue dye. Cell morphological changes were observed by Giemsa staining and light microscopy. Apoptosis was detected by using fluorescence microscopy and caspase-3 activity assay. Both intracellular reactive oxygen species (ROS) and reduced glutathione (GSH) were examined by a microplate-reader method. Results showed that ZnO NPs (0.011.1g/mL) significantly inhibited proliferation (P < 0.05) and induced substantial apoptosis in 1,TEP-a-2 cells after 4 h of exposure. The intracellular ROS level rose up to 30-40% corresponding to significant depletion (approximately 70-80%) in GSH content in LTEP-a-2 cells (P < 0.05), suggesting that ZnO NPs induced apoptosis mainly through increased ROS production. This study elucidates the toxicological mechanism of ZnO NPs in human pulmonary adenocarcinoma cells and provides reference data for application of nanomaterials in the environment. |
| 文章类型 | Article
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| 收录类别 | SCI
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| 语种 | 英语
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| 关键词[WOS] | OXIDATIVE STRESS
; PULMONARY TOXICITY
; IN-VITRO
; PARTICLES
; PATHWAYS
; BACTERIA
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| 研究领域[WOS] | Biotechnology & Applied Microbiology
; Research & Experimental Medicine
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| WOS记录号 | WOS:000359551100001
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| 引用统计 |
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| 文献类型 | 期刊论文
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| 条目标识符 | http://ir.yic.ac.cn/handle/133337/9284
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| 专题 | 海岸带生物学与生物资源利用重点实验室_海岸带生物学与生物资源保护实验室
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| 通讯作者 | Hu, XK (reprint author), Chinese Acad Sci, Yantal Inst Coastal Zone Res, Key Lab Coastal Biol & Bioresource Utilizat, 17 Chunhui Rd, Yantai 264003, Peoples R China. [email protected] |
推荐引用方式
GB/T 7714 |
Wang, CX,Hu, XK,Gao, Y,et al. ZnO Nanoparticles Treatment Induces Apoptosis by Increasing Intracellular ROS Levels in LTEP-a-2 Cells[J]. BIOMED RESEARCH INTERNATIONAL,2015.
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| APA |
Wang, CX,Hu, XK,Gao, Y,Ji, YL,&Hu, XK .(2015).ZnO Nanoparticles Treatment Induces Apoptosis by Increasing Intracellular ROS Levels in LTEP-a-2 Cells.BIOMED RESEARCH INTERNATIONAL.
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| MLA |
Wang, CX,et al."ZnO Nanoparticles Treatment Induces Apoptosis by Increasing Intracellular ROS Levels in LTEP-a-2 Cells".BIOMED RESEARCH INTERNATIONAL (2015).
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