| amoA gene abundances and nitrification potential rates suggest that benthic ammonia-oxidizing bacteria and not archaea dominate N cycling in the Colne estuary, United Kingdom | |
| Li, Jialin(1,2,3); Nedwell, David B.(1); Beddow, Jessica(1); Dumbrell, Alex J.(1); McKew, Boyd A.(1); Thorpe, Emma L.(1); Whitby, Corinne(1); Whitby, Corinne | |
| 发表期刊 | Applied and Environmental Microbiology
 |
| ISSN | 0099-2240 |
| 2015 | |
| 卷号 | 81期号:1页码:159-165 |
| 关键词 | Gradient Gel-electrophoresis Ribosomal-rna Salinity Gradient Sediments Diversity Community River Denitrification Quantification Proteobacteria |
| DOI | 10.1128/AEM.02654-14 |
| 产权排序 | (1) School of Biological Sciences, University of Essex, Colchester, United Kingdom; (2) College of Environmental Science and Engineering, Ocean University of China, Qingdao, China; (3) Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, Yantai, China |
| 作者部门 | 海岸带生物学与生物资源利用所重点实验室 |
| 英文摘要 | Nitrification, mediated by ammonia-oxidizing bacteria (AOB) and ammonia-oxidizing archaea (AOA), is important in global nitrogen cycling. In estuaries where gradients of salinity and ammonia concentrations occur, there may be differential selections for ammonia-oxidizer populations. The aim of this study was to examine the activity, abundance, and diversity of AOA and AOB in surface oxic sediments of a highly nutrified estuary that exhibits gradients of salinity and ammonium. AOB and AOA communities were investigated by measuring ammonia monooxygenase (amoA) gene abundance and nitrification potentials both spatially and temporally. Nitrification potentials differed along the estuary and over time, with the greatest nitrification potentials occurring mid-estuary (8.2 mu mol N grams dry weight [gdw](-1) day(-1) in June, increasing to 37.4 mu mol N gdw(-1) day(-1) in January). At the estuary head, the nitrification potential was 4.3 mu mol N gdw(-1) day(-1) in June, increasing to 11.7 mu mol N gdw(-1) day(-1) in January. At the estuary head and mouth, nitrification potentials fluctuated throughout the year. AOB amoA gene abundances were significantly greater (by 100-fold) than those of AOA both spatially and temporally. Nitrosomonas spp. were detected along the estuary by denaturing gradient gel electrophoresis (DGGE) band sequence analysis. In conclusion, AOB dominated over AOA in the estuarine sediments, with the ratio of AOB/AOA amoA gene abundance increasing from the upper (freshwater) to lower (marine) regions of the Colne estuary. These findings suggest that in this nutrified estuary, AOB (possibly Nitrosomonas spp.) were of major significance in nitrification. |
| 文章类型 | 期刊论文 |
| 资助机构 | Biotechnology & Applied Microbiology ; Microbiology |
| 收录类别 | SCI |
| 语种 | 英语 |
| 研究领域[WOS] | Chinese Scholarship Council Fund, Natural Environment Research Council (NERC) [NE/H525289/1] ; University of Essex |
| WOS记录号 | WOS:000347376200019 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.yic.ac.cn/handle/133337/8847 |
| 专题 | 海岸带生物学与生物资源利用重点实验室_海岸带生物学与生物资源保护实验室 |
| 通讯作者 | Whitby, Corinne |
| 推荐引用方式 GB/T 7714 | Li, Jialin,Nedwell, David B.,Beddow, Jessica,et al. amoA gene abundances and nitrification potential rates suggest that benthic ammonia-oxidizing bacteria and not archaea dominate N cycling in the Colne estuary, United Kingdom[J]. Applied and Environmental Microbiology,2015,81(1):159-165. |
| APA | Li, Jialin.,Nedwell, David B..,Beddow, Jessica.,Dumbrell, Alex J..,McKew, Boyd A..,...&Whitby, Corinne.(2015).amoA gene abundances and nitrification potential rates suggest that benthic ammonia-oxidizing bacteria and not archaea dominate N cycling in the Colne estuary, United Kingdom.Applied and Environmental Microbiology,81(1),159-165. |
| MLA | Li, Jialin,et al."amoA gene abundances and nitrification potential rates suggest that benthic ammonia-oxidizing bacteria and not archaea dominate N cycling in the Colne estuary, United Kingdom".Applied and Environmental Microbiology 81.1(2015):159-165. |
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