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| A potentiometric biosensing system based on an isolated degrading bacterium Klebsiella sp MP-6 for the determination of methyl parathion | |
Geng, Fangfang1,2; Ding, Jiawang2; Jia, Cuiying1; Ding, Baojun1; Qin, Wei2 ; Ding, BJ (reprint author), Dalian Univ Technol, Sch Chem, Dalian 116024, Liaoning, Peoples R China. [email protected]; [email protected]
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| 发表期刊 | RSC ADVANCES
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| ISSN | 2046-2069 |
| 2015 | |
| 卷号 | 5期号:43页码:34475-34480 |
| 关键词 | Molecularly Imprinted Polymer Organophosphate Nerve Agents Solid-phase Extraction Multiwalled Carbon Nanotubes Liquid-chromatography Microbial Biosensor P-nitrophenol Pesticides Sensor Electrode |
| DOI | 10.1039/c5ra00263j |
| 产权排序 | [Geng, Fangfang; Jia, Cuiying; Ding, Baojun] Dalian Univ Technol, Sch Chem, Dalian 116024, Liaoning, Peoples R China; [Geng, Fangfang; Ding, Jiawang; Qin, Wei] Chinese Acad Sci, Yantai Inst Coastal Zone Res YIC, Key Lab Coastal Environm Proc & Ecol Remediat, Shandong Prov Key Lab Coastal Environm Proc,YICCA, Yantai 264003, Shandong, Peoples R China |
| 作者部门 | 中科院海岸带环境过程与生态修复重点实验室 |
| 英文摘要 | A potentiometric sensing system for the sensitive and selective detection of methyl parathion (MP) is described in this paper. The system is based on a degrading bacterium Klebsiella sp. MP-6 as recognition element and a polymeric membrane anion-sensitive electrode as a transducer. Klebsiella sp. MP-6 can be isolated from long-term organophosphorus pesticide contaminated soils, which is capable of biodegrading MP to produce p-nitrophenol. The product can be deprotonated under basic conditions and thus detected by using the anion exchanger based membrane electrode. The bioreactor is prepared by packing the bacterial cells between two polyether sulfone membranes placed in a holder. Molecularly imprinted solid-phase extraction using the MP imprinted polymer as a sorbent enables accumulation and separation of MP from real samples. Under the optimized experimental conditions, the potential response of the biosensing system is linear with the MP concentration in the range of 5-100 nM. The detection limit is 1 nM. The electrode exhibits an excellent selectivity towards other organophosphorus pesticides. The sensing system has been evaluated with spiked water samples and shows good recovery and high accuracy. This methodology is promising to develop potentiometric sensors for detecting organophosphorus pesticides at trace levels in the environment.; A potentiometric sensing system for the sensitive and selective detection of methyl parathion (MP) is described in this paper. The system is based on a degrading bacterium Klebsiella sp. MP-6 as recognition element and a polymeric membrane anion-sensitive electrode as a transducer. Klebsiella sp. MP-6 can be isolated from long-term organophosphorus pesticide contaminated soils, which is capable of biodegrading MP to produce p-nitrophenol. The product can be deprotonated under basic conditions and thus detected by using the anion exchanger based membrane electrode. The bioreactor is prepared by packing the bacterial cells between two polyether sulfone membranes placed in a holder. Molecularly imprinted solid-phase extraction using the MP imprinted polymer as a sorbent enables accumulation and separation of MP from real samples. Under the optimized experimental conditions, the potential response of the biosensing system is linear with the MP concentration in the range of 5-100 nM. The detection limit is 1 nM. The electrode exhibits an excellent selectivity towards other organophosphorus pesticides. The sensing system has been evaluated with spiked water samples and shows good recovery and high accuracy. This methodology is promising to develop potentiometric sensors for detecting organophosphorus pesticides at trace levels in the environment. |
| 文章类型 | Article |
| 资助机构 | Chemistry |
| 收录类别 | SCI |
| 语种 | 英语 |
| 关键词[WOS] | MOLECULARLY IMPRINTED POLYMER ; ORGANOPHOSPHATE NERVE AGENTS ; SOLID-PHASE EXTRACTION ; MULTIWALLED CARBON NANOTUBES ; LIQUID-CHROMATOGRAPHY ; MICROBIAL BIOSENSOR ; P-NITROPHENOL ; PESTICIDES ; SENSOR ; ELECTRODE |
| 研究领域[WOS] | Chemistry |
| WOS记录号 | WOS:000353167500094 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.yic.ac.cn/handle/133337/8667 |
| 专题 | 中国科学院海岸带环境过程与生态修复重点实验室 |
| 通讯作者 | Ding, BJ (reprint author), Dalian Univ Technol, Sch Chem, Dalian 116024, Liaoning, Peoples R China. [email protected]; [email protected] |
| 作者单位 | 1.Dalian Univ Technol, Sch Chem, Dalian 116024, Liaoning, Peoples R China 2.Chinese Acad Sci, Yantai Inst Coastal Zone Res YIC, Key Lab Coastal Environm Proc & Ecol Remediat, Shandong Prov Key Lab Coastal Environm Proc,YICCA, Yantai 264003, Shandong, Peoples R China |
| 推荐引用方式 GB/T 7714 | Geng, Fangfang,Ding, Jiawang,Jia, Cuiying,et al. A potentiometric biosensing system based on an isolated degrading bacterium Klebsiella sp MP-6 for the determination of methyl parathion[J]. RSC ADVANCES,2015,5(43):34475-34480. |
| APA | Geng, Fangfang.,Ding, Jiawang.,Jia, Cuiying.,Ding, Baojun.,Qin, Wei.,[email protected].(2015).A potentiometric biosensing system based on an isolated degrading bacterium Klebsiella sp MP-6 for the determination of methyl parathion.RSC ADVANCES,5(43),34475-34480. |
| MLA | Geng, Fangfang,et al."A potentiometric biosensing system based on an isolated degrading bacterium Klebsiella sp MP-6 for the determination of methyl parathion".RSC ADVANCES 5.43(2015):34475-34480. |
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