Institutional Repository of Key Laboratory of Coastal Zone Environmental Processes, Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences (KLCEP)
| Highly sensitive fluorescence detection of copper ion based on its catalytic oxidation to cysteine indicated by fluorescein isothiocyanate functionalized gold nanoparticles | |
Wang, Shasha1,3; Wang, Xiaokun1,2; Zhang, Zhiyang1,3 ; Chen, Lingxin1; Chen, LX (reprint author), Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Environm Proc & Ecol Remediat, Yantai 264003, Peoples R China. [email protected]
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| 发表期刊 | COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
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| ISSN | 0927-7757 |
| 2015-03-05 | |
| 卷号 | 468页码:333-338 |
| 关键词 | Gold Nanoparticles Fluorescein Isothiocyanate Fluorescence Resonance Energy Transfer Copper Ions Fluorescence |
| DOI | 10.1016/j.colsurfa.2014.12.050 |
| 产权排序 | [Wang, Shasha; Wang, Xiaokun; Zhang, Zhiyang; Chen, Lingxin] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Environm Proc & Ecol Remediat, Yantai 264003, Peoples R China; [Wang, Xiaokun] Qufu Normal Univ, Coll Chem & Chem Engn, Qufu 273165, Peoples R China; [Wang, Shasha; Zhang, Zhiyang] Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 作者部门 | 中科院海岸带环境过程与生态修复重点实验室 |
| 英文摘要 | An innovative fluorescence method for sensitive detection of copper ion (Cu2+) was developed based on fluorescein isothiocyanate functionalized gold nanoparticles (FITC-AuNPs). Due to the stronger binding affinity of isothiocyanate functional group to gold, FITC molecules could adsorb on the surface of AuNPs, forming a simple fluorescence resonance energy transfer (FRET) system, and the fluorescence intensity of FITC was remarkably quenched. Upon adding cysteine, FITC could be displaced from the surface of AuNPs because the formation constant (K-f) of Au-S linkage (K-f(AuS-) = 4 x 10(35)) was much higher than AuSCN linkage (K-f(Au(SCN)(2-)) = 10(23)), leading to the recovery of fluorescence intensity. However, Cu2+ could catalyze O-2 oxidation of cysteine, and the generated disulfide cystine could not remove FITC from AuNPs' surface. Therefore, the recovery of fluorescence intensity was much weaker when compared with that of in the absence of Cu2+. And on the basis of this principle the concentration of Cu2+ could be detected quantitatively. Under optimal conditions, our method exhibited high selectivity toward Cu2+ and provided a good linear relationship in the range of 1.0-17.0 nM with the detection limit of 0.37 nM calculated by 3 sigma/S. Furthermore, complicated synthetic procedures and poor water solubility could be ignored in this proposed fluorescent sensor. (C) 2015 Elsevier B. V. All rights reserved.; An innovative fluorescence method for sensitive detection of copper ion (Cu2+) was developed based on fluorescein isothiocyanate functionalized gold nanoparticles (FITC-AuNPs). Due to the stronger binding affinity of isothiocyanate functional group to gold, FITC molecules could adsorb on the surface of AuNPs, forming a simple fluorescence resonance energy transfer (FRET) system, and the fluorescence intensity of FITC was remarkably quenched. Upon adding cysteine, FITC could be displaced from the surface of AuNPs because the formation constant (K-f) of Au-S linkage (K-f(AuS-) = 4 x 10(35)) was much higher than AuSCN linkage (K-f(Au(SCN)(2-)) = 10(23)), leading to the recovery of fluorescence intensity. However, Cu2+ could catalyze O-2 oxidation of cysteine, and the generated disulfide cystine could not remove FITC from AuNPs' surface. Therefore, the recovery of fluorescence intensity was much weaker when compared with that of in the absence of Cu2+. And on the basis of this principle the concentration of Cu2+ could be detected quantitatively. Under optimal conditions, our method exhibited high selectivity toward Cu2+ and provided a good linear relationship in the range of 1.0-17.0 nM with the detection limit of 0.37 nM calculated by 3 sigma/S. Furthermore, complicated synthetic procedures and poor water solubility could be ignored in this proposed fluorescent sensor. (C) 2015 Elsevier B. V. All rights reserved. |
| 文章类型 | Article |
| 资助机构 | Chemistry |
| 收录类别 | SCI |
| 语种 | 英语 |
| 关键词[WOS] | ULTRASENSITIVE DETECTION ; SPECTROMETRY ; NANORODS ; TRANSPORT ; GRAPHENE ; PROBES ; SENSOR ; ASSAY ; DOTS |
| 研究领域[WOS] | Chemistry |
| WOS记录号 | WOS:000349446500041 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.yic.ac.cn/handle/133337/8642 |
| 专题 | 中国科学院海岸带环境过程与生态修复重点实验室 中国科学院海岸带环境过程与生态修复重点实验室_海岸带环境工程技术研究与发展中心 |
| 通讯作者 | Chen, LX (reprint author), Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Environm Proc & Ecol Remediat, Yantai 264003, Peoples R China. [email protected] |
| 作者单位 | 1.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Environm Proc & Ecol Remediat, Yantai 264003, Peoples R China 2.Qufu Normal Univ, Coll Chem & Chem Engn, Qufu 273165, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Shasha,Wang, Xiaokun,Zhang, Zhiyang,et al. Highly sensitive fluorescence detection of copper ion based on its catalytic oxidation to cysteine indicated by fluorescein isothiocyanate functionalized gold nanoparticles[J]. COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS,2015,468:333-338. |
| APA | Wang, Shasha,Wang, Xiaokun,Zhang, Zhiyang,Chen, Lingxin,&Chen, LX .(2015).Highly sensitive fluorescence detection of copper ion based on its catalytic oxidation to cysteine indicated by fluorescein isothiocyanate functionalized gold nanoparticles.COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS,468,333-338. |
| MLA | Wang, Shasha,et al."Highly sensitive fluorescence detection of copper ion based on its catalytic oxidation to cysteine indicated by fluorescein isothiocyanate functionalized gold nanoparticles".COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS 468(2015):333-338. |
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