| Analysis of Phenolic Acids of Jerusalem Artichoke (Helianthus tuberosus L.) Responding to Salt-Stress by Liquid Chromatography/Tandem Mass Spectrometry | |
| Chen, Fujia1; Long, Xiaohua1; Liu, Zhaopu1; Shao, Hongbo2,3; Liu, Ling1; Shao, HB (reprint author), Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Biol & Bioresources Utilizat, Yantai 264003, Peoples R China. [email protected]; [email protected] | |
| 发表期刊 | SCIENTIFIC WORLD JOURNAL
 |
| ISSN | 1537-744X |
| 2014 | |
| 页码 | 568043 |
| 关键词 | Chlorogenic Acids Antioxidant Properties Rat Plasma Identification Hplc Constituents Dad Extract Fruit Ms |
| DOI | 10.1155/2014/568043 |
| 产权排序 | [Chen, Fujia; Long, Xiaohua; Liu, Zhaopu; Liu, Ling] Nanjing Agr Univ, Coll Resources & Environm Sci, Key Lab Marine Biol Jiangsu Prov, Nanjing 210095, Jiangsu, Peoples R China; [Shao, Hongbo] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Biol & Bioresources Utilizat, Yantai 264003, Peoples R China; [Shao, Hongbo] Jiangsu Acad Agr Sci, Nanjing 210014, Jiangsu, Peoples R China |
| 作者部门 | 海岸带生物学与生物资源利用所重点实验室 |
| 英文摘要 | Plant phenolics can have applications in pharmaceutical and other industries. To identify and quantify the phenolic compounds in Helianthus tuberosus leaves, qualitative analysis was performed by a reversed phase high-performance liquid chromatography coupled with tandem mass spectrometry (HPLC-MS/MS) and quantitative analysis by HPLC. Ten chlorogenic acids (CGAs) were identified (3-o-caffeoylquinic acid, two isomers of caffeoylquinic acid, caffeic acid, p-coumaroyl-quinic acid, feruloylquinic acid, 3,4-dicaffeoyquinic acid, 3,5-dicaffeoylquinic acid, 1,5-dicaffeoylquinic acid, and 4,5-dicaffeoylquinic acid) by comparing their retention times, UV-Vis absorption spectra, and MS/MS spectra with standards. In addition, four other phenolic compounds, including caffeoyl glucopyranose, isorhamnetin glucoside, kaempferol glucuronide, and kaempferol-3-o-glucoside, were tentatively identified in Helianthus tuberosus leaves for the first time. The 3-o-caffeoylquinic acid (7.752mg/g DW), 4,5-dicaffeoylquinic acid (5.633mg/g DW), and 3,5-dicaffeoylquinic acid (4.900mg/g DW) were the major phenolic compounds in leaves of Helianthus tuberosus cultivar NanYu in maturity. The variations in phenolic concentrations and proportions in Helianthus tuberosus leaves were influenced by genotype and plant growth stage. Cultivar NanYu had the highest concentration of phenolic compounds, in particular 3-o-caffeoylquinic acid and 4,5-dicaffeoylquinic acid compared with the other genotypes (wild accession and QingYu). Considering various growth stages, the concentration of total phenolics in cultivar NanYu was higher at flowering stage (5.270 mg/g DW) than at budding and tuber swelling stages. Cultivar NanYu of Helianthus tuberosus is a potential source of natural phenolics that may play an important role in the development of pharmaceuticals.; Plant phenolics can have applications in pharmaceutical and other industries. To identify and quantify the phenolic compounds in Helianthus tuberosus leaves, qualitative analysis was performed by a reversed phase high-performance liquid chromatography coupled with tandem mass spectrometry (HPLC-MS/MS) and quantitative analysis by HPLC. Ten chlorogenic acids (CGAs) were identified (3-o-caffeoylquinic acid, two isomers of caffeoylquinic acid, caffeic acid, p-coumaroyl-quinic acid, feruloylquinic acid, 3,4-dicaffeoyquinic acid, 3,5-dicaffeoylquinic acid, 1,5-dicaffeoylquinic acid, and 4,5-dicaffeoylquinic acid) by comparing their retention times, UV-Vis absorption spectra, and MS/MS spectra with standards. In addition, four other phenolic compounds, including caffeoyl glucopyranose, isorhamnetin glucoside, kaempferol glucuronide, and kaempferol-3-o-glucoside, were tentatively identified in Helianthus tuberosus leaves for the first time. The 3-o-caffeoylquinic acid (7.752mg/g DW), 4,5-dicaffeoylquinic acid (5.633mg/g DW), and 3,5-dicaffeoylquinic acid (4.900mg/g DW) were the major phenolic compounds in leaves of Helianthus tuberosus cultivar NanYu in maturity. The variations in phenolic concentrations and proportions in Helianthus tuberosus leaves were influenced by genotype and plant growth stage. Cultivar NanYu had the highest concentration of phenolic compounds, in particular 3-o-caffeoylquinic acid and 4,5-dicaffeoylquinic acid compared with the other genotypes (wild accession and QingYu). Considering various growth stages, the concentration of total phenolics in cultivar NanYu was higher at flowering stage (5.270 mg/g DW) than at budding and tuber swelling stages. Cultivar NanYu of Helianthus tuberosus is a potential source of natural phenolics that may play an important role in the development of pharmaceuticals. |
| 文章类型 | Article |
| 资助机构 | Science & Technology - Other Topics |
| 收录类别 | SCI |
| 语种 | 英语 |
| 关键词[WOS] | CHLOROGENIC ACIDS ; ANTIOXIDANT PROPERTIES ; RAT PLASMA ; IDENTIFICATION ; HPLC ; CONSTITUENTS ; DAD ; EXTRACT ; FRUIT ; MS |
| 研究领域[WOS] | Science & Technology - Other Topics |
| WOS记录号 | WOS:000343479100001 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.yic.ac.cn/handle/133337/8568 |
| 专题 | 海岸带生物学与生物资源利用重点实验室_海岸带生物学与生物资源保护实验室 |
| 通讯作者 | Shao, HB (reprint author), Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Biol & Bioresources Utilizat, Yantai 264003, Peoples R China. [email protected]; [email protected] |
| 作者单位 | 1.Nanjing Agr Univ, Coll Resources & Environm Sci, Key Lab Marine Biol Jiangsu Prov, Nanjing 210095, Jiangsu, Peoples R China 2.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Biol & Bioresources Utilizat, Yantai 264003, Peoples R China 3.Jiangsu Acad Agr Sci, Nanjing 210014, Jiangsu, Peoples R China |
| 推荐引用方式 GB/T 7714 | Chen, Fujia,Long, Xiaohua,Liu, Zhaopu,et al. Analysis of Phenolic Acids of Jerusalem Artichoke (Helianthus tuberosus L.) Responding to Salt-Stress by Liquid Chromatography/Tandem Mass Spectrometry[J]. SCIENTIFIC WORLD JOURNAL,2014:568043. |
| APA | Chen, Fujia.,Long, Xiaohua.,Liu, Zhaopu.,Shao, Hongbo.,Liu, Ling.,[email protected].(2014).Analysis of Phenolic Acids of Jerusalem Artichoke (Helianthus tuberosus L.) Responding to Salt-Stress by Liquid Chromatography/Tandem Mass Spectrometry.SCIENTIFIC WORLD JOURNAL,568043. |
| MLA | Chen, Fujia,et al."Analysis of Phenolic Acids of Jerusalem Artichoke (Helianthus tuberosus L.) Responding to Salt-Stress by Liquid Chromatography/Tandem Mass Spectrometry".SCIENTIFIC WORLD JOURNAL (2014):568043. |
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