| Eichhornia crassipes Cleans Wetlands by Enhancing the Nitrogen Removal and Modulating Denitrifying Bacteria Community | |
| Yi, Neng1,2; Gao, Yan2; Long, Xiao-hua1; Zhang, Zhi-yong2; Guo, Jun-yao2; Shao, Hong-bo1,3; Zhang, Zhen-hua2; Yan, Shao-hua1,2; Yan, SH (reprint author), Nanjing Agr Univ, Key Lab Marine Biol Jiangsu Prov, Nanjing 210095, Jiangsu, Peoples R China. [email protected]; [email protected] | |
| 发表期刊 | CLEAN-SOIL AIR WATER
 |
| ISSN | 1863-0650 |
| 2014-05-01 | |
| 卷号 | 42期号:5页码:664-673 |
| 关键词 | Denitrification Gene Dgge 15n Stable Isotope Tracing Qpcr Water Hyacinth Cleaning |
| DOI | 10.1002/clen.201300211 |
| 产权排序 | [Yi, Neng; Long, Xiao-hua; Shao, Hong-bo; Yan, Shao-hua] Nanjing Agr Univ, Key Lab Marine Biol Jiangsu Prov, Nanjing 210095, Jiangsu, Peoples R China; [Yi, Neng; Gao, Yan; Zhang, Zhi-yong; Guo, Jun-yao; Zhang, Zhen-hua; Yan, Shao-hua] Jiangsu Acad Agr Sci, Inst Agr Resources & Environm, Nanjng, Peoples R China; [Shao, Hong-bo] Chinese Acad Sci, Yantai Inst Coastal Zone Res YIC, Key Lab Coastal Biol & Bioresources Utilizat, Yantai, Peoples R China |
| 作者部门 | 海岸带生物学与生物资源利用所重点实验室 |
| 英文摘要 | The role of floating macrophytes on modulating the microbial nitrogen removal is not well understood. In this study, the cultivation of Eichhornia crassipes in eutrophic water may affect the nitrogen (N) fate by modulating the denitrifying bacteria diversity and abundance. The gaseous N losses via denitrification were estimated by 15N stable isotope tracing and the diversity and abundance of denitrifying genes (nirS, nirK, and nosZ) were investigated by molecular tools. The denaturing gradient gel electrophoresis (DGGE) profiles showed that the diversity of denitrifying genes in the treatments with E. crassipes was significantly higher than that in the treatment without E. crassipes. The real-time PCR (qPCR) results showed the trend of denitrifier abundance in the entire system was in the order of N-ER (nitrate with just root of E. crassipes) and A-ER (ammonia with just root of E. crassipes)>N-R (nitrate with E. crassipes) and A-R (ammonia with E. crassipes)>N-W (nitrate without plant) and A-W (ammonia without plant). The gaseous 15N losses via denitrification were significantly and positively related to the abundance of nirK, nirS, and nosZ genes. The results indicated that cultivation of E. crassipes in eutrophic water could increase the diversity and abundance of denitrifying bacteria, resulting in more N being removed as gases via denitrification.; The role of floating macrophytes on modulating the microbial nitrogen removal is not well understood. In this study, the cultivation of Eichhornia crassipes in eutrophic water may affect the nitrogen (N) fate by modulating the denitrifying bacteria diversity and abundance. The gaseous N losses via denitrification were estimated by 15N stable isotope tracing and the diversity and abundance of denitrifying genes (nirS, nirK, and nosZ) were investigated by molecular tools. The denaturing gradient gel electrophoresis (DGGE) profiles showed that the diversity of denitrifying genes in the treatments with E. crassipes was significantly higher than that in the treatment without E. crassipes. The real-time PCR (qPCR) results showed the trend of denitrifier abundance in the entire system was in the order of N-ER (nitrate with just root of E. crassipes) and A-ER (ammonia with just root of E. crassipes)>N-R (nitrate with E. crassipes) and A-R (ammonia with E. crassipes)>N-W (nitrate without plant) and A-W (ammonia without plant). The gaseous 15N losses via denitrification were significantly and positively related to the abundance of nirK, nirS, and nosZ genes. The results indicated that cultivation of E. crassipes in eutrophic water could increase the diversity and abundance of denitrifying bacteria, resulting in more N being removed as gases via denitrification. |
| 文章类型 | Article |
| 资助机构 | Environmental Sciences & Ecology ; Marine & Freshwater Biology ; Water Resources |
| 收录类别 | SCI |
| 语种 | 英语 |
| 关键词[WOS] | CONSTRUCTED WETLANDS ; OXIDE REDUCTASE ; WATER-QUALITY ; SUBMERGED MACROPHYTES ; DENITRIFICATION GENES ; AGRICULTURAL SOIL ; N2O EMISSIONS ; FRESH-WATER ; NOSZ GENES ; NIRK |
| 研究领域[WOS] | Environmental Sciences & Ecology ; Marine & Freshwater Biology ; Water Resources |
| WOS记录号 | WOS:000337514900017 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.yic.ac.cn/handle/133337/8551 |
| 专题 | 海岸带生物学与生物资源利用重点实验室_海岸带生物学与生物资源保护实验室 |
| 通讯作者 | Yan, SH (reprint author), Nanjing Agr Univ, Key Lab Marine Biol Jiangsu Prov, Nanjing 210095, Jiangsu, Peoples R China. [email protected]; [email protected] |
| 作者单位 | 1.Nanjing Agr Univ, Key Lab Marine Biol Jiangsu Prov, Nanjing 210095, Jiangsu, Peoples R China 2.Jiangsu Acad Agr Sci, Inst Agr Resources & Environm, Nanjng, Peoples R China 3.Chinese Acad Sci, Yantai Inst Coastal Zone Res YIC, Key Lab Coastal Biol & Bioresources Utilizat, Yantai, Peoples R China |
| 推荐引用方式 GB/T 7714 | Yi, Neng,Gao, Yan,Long, Xiao-hua,et al. Eichhornia crassipes Cleans Wetlands by Enhancing the Nitrogen Removal and Modulating Denitrifying Bacteria Community[J]. CLEAN-SOIL AIR WATER,2014,42(5):664-673. |
| APA | Yi, Neng.,Gao, Yan.,Long, Xiao-hua.,Zhang, Zhi-yong.,Guo, Jun-yao.,[email protected].(2014).Eichhornia crassipes Cleans Wetlands by Enhancing the Nitrogen Removal and Modulating Denitrifying Bacteria Community.CLEAN-SOIL AIR WATER,42(5),664-673. |
| MLA | Yi, Neng,et al."Eichhornia crassipes Cleans Wetlands by Enhancing the Nitrogen Removal and Modulating Denitrifying Bacteria Community".CLEAN-SOIL AIR WATER 42.5(2014):664-673. |
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