滨海盐渍化土壤中蓝细菌多样性及分布

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	滨海盐渍化土壤中蓝细菌多样性及分布
其他题名	Diversity and distribution of cyanobacteria in coastal saline soils
	李寒; 张晓黎; 郭晓红; 宋延静; 龚骏; 龚骏,[email protected]
发表期刊	微生物学通报
ISSN	0253-2654
	2015
卷号	42 期号:5 页码:957-967
关键词	蓝细菌盐度梯度群落结构多样性丰度
产权排序	中国科学院烟台海岸带研究所微型生物生态与物质循环实验室;中国科学院大学;
作者部门	海岸带生物学与生物资源利用所重点实验室
英文摘要	【目的】蓝细菌在贫瘠土壤的固氮、固碳中发挥着重要作用,然而土壤盐渍化对蓝细菌多样性及群落结构的影响还不清楚。本研究以莱州湾南岸及黄河口海水入侵盐渍化土壤为例,研究蓝细菌的多样性、群落结构及丰度沿盐度梯度的分布情况。【方法】利用自动核糖体间隔基因分析(ARISA)技术与群落相似性分析(ANOSIM)探究蓝细菌群落的差异与空间分布格局;通过16S r RNA基因克隆文库、测序与系统进化分析解析了3个典型盐度梯度样品中蓝细菌的群落组成;实时定量PCR测定蓝细菌16S r RNA拷贝数(丰度);BEST多元分析探寻影响蓝细菌分布的主要环境因子。【结果】蓝细菌在莱州湾南岸盐土中分布广泛,其群落结构在低(...
中文摘要	Little is known about the diversity and distribution of cyanobacteria in salinized soils. In this study, we determined soil properties （pH, salinity and water contents, ammonium, nitrite, nitrate, total organic carbon and nitrogen）, and investigated the moleculardiversity and distribution pattems of soil cyanobacteria at 12 sites near the mouth of Yellow River and in the southem coastal plain of Laizhou Bay （Shandong province）, typical salinized coastal regions in China. Salinity gradients were devided into three ranges： low （0.63%-1.27%）, medium （1.55%-2.00%）, and high saline （2.39%-5.11%）. [Methods] Automated ribosomal intergenic spacer analysis （ARISA）, clone libraries and real-time quantitative PCR were used to determine the cyanobacterial community structure, composition and abundance, respectively. [Results] Based on ARISA fingerprints, we found cyanobacterial phylotype richness increased with salinity; multiple dimensional scaling and hypothesis testing （ANOSIM） indicated that the community structure of cyanobacteria generally clustered by salinity （P=-0.03） more than by water content （P=0.09）. Biota-Environment correlation （BEST） analysis further revealed that a combination of two factors, salinity and water content of soils, could mostly explain the changes in cyanobacterial community structure （P=0.02）. Clone libraries of 16S rRNA gene were constructed for 3 representative samples. Sequencing and classification following phylogenetic analysis showed that Halomicronema and Acaryochloris dominated in the low saline samples, whereas Leptolyngbya was the most abundant in both medium and high saline soils. Another two taxa, Arthrospira and Geitlerinema, were only found in the samples of low salinity, while Oscillatoria was detected once from the high salinity. Cyanbacterial 16S rRNA genes were much more abundant in low salinity （2.14×10^5 copies/g dry soil） than in medium （1.25×10^5 copies/g dry soil） and high salinity samples （1.20× 10^5 copies/g dry soil）. [Conclusion] This study demonstrates that salinity shapes the diversity, community structure and abundance of cyanobacteria in the saline soils, which has implications for carbon and nitrogen cycling contributed by these microorganisms in coastal terrestrial ecosystems.
文章类型	期刊论文
资助机构	中科院百人计划项目 ; 国家自然科学基金青年科学基金项目(No.41206155) ; 烟台市双百计划人才项目
收录类别	CSCD
语种	中文
CSCD记录号	CSCD:5436702
引用统计
文献类型	期刊论文
条目标识符	http://ir.yic.ac.cn/handle/133337/8365
专题	海岸带生物学与生物资源利用重点实验室_海岸带生物学与生物资源保护实验室
通讯作者	龚骏,[email protected]
推荐引用方式 GB/T 7714	李寒,张晓黎,郭晓红,等. 滨海盐渍化土壤中蓝细菌多样性及分布[J]. 微生物学通报,2015,42(5):957-967.
APA	李寒,张晓黎,郭晓红,宋延静,龚骏,&龚骏,[email protected].(2015).滨海盐渍化土壤中蓝细菌多样性及分布.微生物学通报,42(5),957-967.
MLA	李寒,et al."滨海盐渍化土壤中蓝细菌多样性及分布".微生物学通报 42.5(2015):957-967.

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