| 大型海藻内共生真菌次生代谢产物中抑制水华蓝藻物质的分离与活性 |
| 其他题名 | Identification of Compounds with Allelopathy on Bloom Microalgae from the Secondary Metabolites of Endophytic Fungi Isolated from Marine Macroalgae and the Algicidal Activities
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| 左进城; 苗凤萍 ; 王爱云; 胡德昌
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| 发表期刊 | 植物科学学报
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| ISSN | 2095-0837
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| 2014
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| 卷号 | 32期号:5页码:509-516 |
| 关键词 | 内共生真菌
抑藻物质
水华鱼腥藻
铜绿微囊藻
化感作用
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| 产权排序 | 鲁东大学生命科学学院; 中国科学院烟台海岸带研究所 |
| 作者部门 | 海岸带生物学与生物资源利用所重点实验室
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| 英文摘要 | 为获得能明显抑制水华蓝藻的化感物质,本研究从28株大型海藻内共生真菌中筛选出了4株菌株,其粗提物能显著抑制水华鱼腥藻(Anabaena flos-aquae)和铜绿微囊藻(Microcystis aeruginosa)的生长。对这4株真菌扩大培养后,检测了从其次生代谢产物中分离出的32种单体化合物的抑藻活性。其中,化合物sterigmatocystin、(2'E,4'E,6'E)-6-(1'-carboxyocta-2',4',6'-triene)-11,12-epoxy-9,11-dihydroxydrim-7-ene、 6,8-di-O-methylnidurufin和aversin对水华鱼腥藻的抑制率显著大于50%(P < 0.05),24 h半数有效浓度(EC_(50,24h))分别为6.865、4.302、1.824、2.319 μg/mL;化合物sterigmatocystin、indol-3-carboxaldehyde和(2'E,4'E,6'E)-6-(1'-carboxyocta-2',4',6'-triene)-11,12-epoxy-9,11-dihydroxydrim-7-ene对铜绿微囊藻的抑制率显著大于50%(P < 0.05),EC_(50,24h)分别为4.167、5.374、8.386 μg/mL。 |
| 中文摘要 | To obtain compounds with allelopathy on bloom microalgae, 28 strains of marine macroalgae-endophytic fungi were tested, and four strains in which crude extracts of the secondary metabolites showed apparent allelopathy on Anabaena flos-aquae and Microcystis aeruginosa were obtained. The four strains were cultured in 15 L of PDB culture and the algicidal activities of 32 compounds isolated from the secondary metabolites were examined. The inhibition ratios of sterigmatocystin, (2'E,4'E,6'E)-6-(1'-carboxyocta-2',4',6'-triene)-11,12-epoxy-9,11-dihydroxydrim-7-ene, 6,8-di-O-methylnidurufin and aversin on A. flos-aquae were all significantly higher than 50% (P < 0.05), and the EC_(50,24h )values were 6.865 mug/mL, 4.302 mug/mL, 1.824 mug/mL and 2.319 mug/mL, respectively. The inhibition ratios of sterigmatocystin,(2'E,4'E,6'E)-6-(1'-carboxyocta-2',4',6'-triene)-11,12-epoxy-9,11-dihydroxydrim-7-ene and indol-3-carboxaldehyde on M. aeruginosa were all significantly higher than 50% (P < 0.05), and the EC_(50,24h) values were 4.167 mug/mL, 8.386 mug/mL and 5.374 mug/mL, respectively. |
| 文章类型 | 期刊论文
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| 资助机构 | 国家"十二五"水专项,国家青年科学基金项目,山东省自然科学基金,鲁东大学博士基金
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| 收录类别 | CSCD
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| 语种 | 中文
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| CSCD记录号 | CSCD:5272673
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| 引用统计 |
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| 文献类型 | 期刊论文
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| 条目标识符 | http://ir.yic.ac.cn/handle/133337/8349
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| 专题 | 海岸带生物学与生物资源利用重点实验室_海岸带生物学与生物资源保护实验室
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推荐引用方式
GB/T 7714 |
左进城,苗凤萍,王爱云,等. 大型海藻内共生真菌次生代谢产物中抑制水华蓝藻物质的分离与活性[J]. 植物科学学报,2014,32(5):509-516.
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| APA |
左进城,苗凤萍,王爱云,&胡德昌.(2014).大型海藻内共生真菌次生代谢产物中抑制水华蓝藻物质的分离与活性.植物科学学报,32(5),509-516.
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| MLA |
左进城,et al."大型海藻内共生真菌次生代谢产物中抑制水华蓝藻物质的分离与活性".植物科学学报 32.5(2014):509-516.
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