Molecular Cloning, Sequence Analysis and Phylogeny of First Caudata G-type Lysozyme in Axolotl (Ambystoma mexicanum)

	Molecular Cloning, Sequence Analysis and Phylogeny of First Caudata G-type Lysozyme in Axolotl (Ambystoma mexicanum)
其他题名	Molecularly imprinted nanoparticles based potentiometric sensor with a nanomolar detection limit.pdf
	Yu, Haining 1,2; Gao, Jiuxiang 1; Lu, Yiling 1; Guang, Huijuan 1; Cai, Shasha 1; Zhang, Songyan 1; Wang, Yipeng3 ; Yu, HN (reprint author), Dalian Univ Technol, Dept Biosci & Biotechnol, Dalian 116023, Peoples R China. [email protected]
发表期刊	ZOOLOGICAL SCIENCE
ISSN	0289-0003
	2013-11-01
卷号	30 期号:11 页码:938-943
关键词	Ambystoma Mexicanum Caudate Amphibian Lysozyme Molecular Cloning Phylogeny
产权排序	[Yu, Haining; Gao, Jiuxiang; Lu, Yiling; Guang, Huijuan; Cai, Shasha; Zhang, Songyan] Dalian Univ Technol, Dept Biosci & Biotechnol, Dalian 116023, Peoples R China; [Yu, Haining] Hebei Normal Univ, Coll Life Sci, Shijiazhuang 050016, Peoples R China; [Wang, Yipeng] Chinese Acad Sci, Yantai Inst Coastal Zone Reseach, Yantai 264003, Shandong, Peoples R China
英文摘要	Lysozymes are key proteins that play important roles in innate immune defense in many animal phyla by breaking down the bacterial cell-walls. In this study, we report the molecular cloning, sequence analysis and phylogeny of the first caudate amphibian g-lysozyme: a full-length spleen cDNA library from axolotl (Ambystoma mexicanum). A goose-type (g-lysozyme) EST was identified and the full-length cDNA was obtained using RACE-PCR. The axolotl g-lysozyme sequence represents an open reading frame for a putative signal peptide and the mature protein composed of 184 amino acids. The calculated molecular mass and the theoretical isoelectric point (pI) of this mature protein are 21523.0 Da and 4.37, respectively. Expression of g-lysozyme mRNA is predominantly found in skin, with lower levels in spleen, liver, muscle, and lung. Phylogenetic analysis revealed that caudate amphibian g-lysozyme had distinct evolution pattern for being juxtaposed with not only anura amphibian, but also with the fish, bird and mammal. Although the first complete cDNA sequence for caudate amphibian g-lysozyme is reported in the present study, clones encoding axolotl's other functional immune molecules in the full-length cDNA library will have to be further sequenced to gain insight into the fundamental aspects of antibacterial mechanisms in caudate.; Lysozymes are key proteins that play important roles in innate immune defense in many animal phyla by breaking down the bacterial cell-walls. In this study, we report the molecular cloning, sequence analysis and phylogeny of the first caudate amphibian g-lysozyme: a full-length spleen cDNA library from axolotl (Ambystoma mexicanum). A goose-type (g-lysozyme) EST was identified and the full-length cDNA was obtained using RACE-PCR. The axolotl g-lysozyme sequence represents an open reading frame for a putative signal peptide and the mature protein composed of 184 amino acids. The calculated molecular mass and the theoretical isoelectric point (pI) of this mature protein are 21523.0 Da and 4.37, respectively. Expression of g-lysozyme mRNA is predominantly found in skin, with lower levels in spleen, liver, muscle, and lung. Phylogenetic analysis revealed that caudate amphibian g-lysozyme had distinct evolution pattern for being juxtaposed with not only anura amphibian, but also with the fish, bird and mammal. Although the first complete cDNA sequence for caudate amphibian g-lysozyme is reported in the present study, clones encoding axolotl's other functional immune molecules in the full-length cDNA library will have to be further sequenced to gain insight into the fundamental aspects of antibacterial mechanisms in caudate.
文章类型	Article
资助机构	Chinese National Natural Science Foundation [41076098, 41206153]
收录类别	SCI
语种	英语
关键词[WOS]	GOOSE-TYPE LYSOZYME ; TAPES-JAPONICA LYSOZYME ; C-TYPE ; RECOMBINANT PROTEIN ; ALPHA-LACTALBUMIN ; LYTIC ACTIVITY ; EXPRESSION ; EVOLUTION ; CDNA ; GENE
研究领域[WOS]	Zoology
WOS记录号	WOS:000326246300008
引用统计	被引频次：9[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.yic.ac.cn/handle/133337/7062
专题	中国科学院烟台海岸带研究所知识产出海岸带生物学与生物资源利用重点实验室_海岸带生物学与生物资源保护实验室
通讯作者	Yu, HN (reprint author), Dalian Univ Technol, Dept Biosci & Biotechnol, Dalian 116023, Peoples R China. [email protected]
作者单位	1.Dalian Univ Technol, Dept Biosci & Biotechnol, Dalian 116023, Peoples R China 2.Hebei Normal Univ, Coll Life Sci, Shijiazhuang 050016, Peoples R China 3.Chinese Acad Sci, Yantai Inst Coastal Zone Reseach, Yantai 264003, Shandong, Peoples R China
推荐引用方式 GB/T 7714	Yu, Haining,Gao, Jiuxiang,Lu, Yiling,et al. Molecular Cloning, Sequence Analysis and Phylogeny of First Caudata G-type Lysozyme in Axolotl (Ambystoma mexicanum)[J]. ZOOLOGICAL SCIENCE,2013,30(11):938-943.
APA	Yu, Haining.,Gao, Jiuxiang.,Lu, Yiling.,Guang, Huijuan.,Cai, Shasha.,...&Yu, HN .(2013).Molecular Cloning, Sequence Analysis and Phylogeny of First Caudata G-type Lysozyme in Axolotl (Ambystoma mexicanum).ZOOLOGICAL SCIENCE,30(11),938-943.
MLA	Yu, Haining,et al."Molecular Cloning, Sequence Analysis and Phylogeny of First Caudata G-type Lysozyme in Axolotl (Ambystoma mexicanum)".ZOOLOGICAL SCIENCE 30.11(2013):938-943.