| A new model for electron flow during anaerobic digestion: direct interspecies electron transfer to Methanosaeta for the reduction of carbon dioxide to methane | |
Rotaru, Amelia-Elena; Shrestha, Pravin Malta; Liu, Fanghua ; Shrestha, Minita; Shrestha, Devesh; Embree, Mallory; Zengler, Karsten; Wardman, Colin; Nevin, Kelly P.; Lovley, Derek R.; Rotaru, AE (reprint author), Univ Massachusetts, Dept Microbiol, Amherst, MA 01003 USA. [email protected]
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| 发表期刊 | ENERGY & ENVIRONMENTAL SCIENCE
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| ISSN | 1754-5692 |
| 2014-01 | |
| 卷号 | 7期号:1页码:408-415 |
| 关键词 | Rice Field Soil Geobacter-sulfurreducens Acetate Rna Microorganisms Bacteria Hydrogen Formate Identification Association |
| 产权排序 | [Rotaru, Amelia-Elena; Shrestha, Pravin Malta; Liu, Fanghua; Shrestha, Minita; Shrestha, Devesh; Wardman, Colin; Nevin, Kelly P.; Lovley, Derek R.] Univ Massachusetts, Dept Microbiol, Amherst, MA 01003 USA; [Liu, Fanghua] Yantai Inst Coastal Res, Yantai, Peoples R China; [Embree, Mallory; Zengler, Karsten] Univ Calif San Diego, La Jolla, CA 92093 USA |
| 英文摘要 | Anaerobic conversion of organic wastes and biomass to methane is an important bioenergy strategy, which depends on poorly understood mechanisms of interspecies electron transfer to methanogenic microorganisms. Metatranscriptomic analysis of methanogenic aggregates from a brewery wastewater digester, coupled with fluorescence in situ hybridization with specific 16S rRNA probes, revealed that Methanosaeta species were the most abundant and metabolically active methanogens. Methanogens known to reduce carbon dioxide with H2 or formate as the electron donor were rare. Although Methanosaeta have previously been thought to be restricted to acetate as a substrate for methane production, Methanosaeta in the aggregates had a complete complement of genes for the enzymes necessary for the reduction of carbon to methane, and transcript abundance for these genes was high. Furthermore, Geobacter species, the most abundant bacteria in the aggregates, highly expressed genes for ethanol metabolism and for extracellular electron transfer via electrically conductive pili, suggesting that Geobacter and Methanosaeta species were exchanging electrons via direct interspecies electron transfer (DIET). This possibility was further investigated in defined co-cultures of Geobacter metallireducens and Methanosaeta harundinacea which stoichiometrically converted ethanol to methane. Transcriptomic, radiotracer, and genetic analysis demonstrated that M. harundinacea accepted electrons via DIET for the reduction of carbon dioxide to methane. The discovery that Methanosaeta species, which are abundant in a wide diversity of methanogenic environments, are capable of DIET has important implications not only for the functioning of anaerobic digesters, but also for global methane production. |
| 资助机构 | Office of Science (BER), U.S. Department of Energy [DE-SC0004485] |
| 收录类别 | SCI |
| 语种 | 英语 |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.yic.ac.cn/handle/133337/7026 |
| 专题 | 海岸带生物学与生物资源利用重点实验室_海岸带生物学与生物资源保护实验室 |
| 通讯作者 | Rotaru, AE (reprint author), Univ Massachusetts, Dept Microbiol, Amherst, MA 01003 USA. [email protected] |
| 推荐引用方式 GB/T 7714 | Rotaru, Amelia-Elena,Shrestha, Pravin Malta,Liu, Fanghua,et al. A new model for electron flow during anaerobic digestion: direct interspecies electron transfer to Methanosaeta for the reduction of carbon dioxide to methane[J]. ENERGY & ENVIRONMENTAL SCIENCE,2014,7(1):408-415. |
| APA | Rotaru, Amelia-Elena.,Shrestha, Pravin Malta.,Liu, Fanghua.,Shrestha, Minita.,Shrestha, Devesh.,...&Rotaru, AE .(2014).A new model for electron flow during anaerobic digestion: direct interspecies electron transfer to Methanosaeta for the reduction of carbon dioxide to methane.ENERGY & ENVIRONMENTAL SCIENCE,7(1),408-415. |
| MLA | Rotaru, Amelia-Elena,et al."A new model for electron flow during anaerobic digestion: direct interspecies electron transfer to Methanosaeta for the reduction of carbon dioxide to methane".ENERGY & ENVIRONMENTAL SCIENCE 7.1(2014):408-415. |
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