| Gene cloning and expression profile of a novel carotenoid hydroxylase (CYP97C) from the green alga Haematococcus pluvialis | |
Cui, Hongli1,2; Yu, Xiaona3; Wang, Yan1 ; Cui, Yulin1; Li, Xueqin4; Liu, Zhaopu3; Qin, Song1; Qin, S (reprint author), Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Biol & Biol Resources Utilizat, Yantai 264003, Peoples R China. [email protected]; [email protected]
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| 发表期刊 | JOURNAL OF APPLIED PHYCOLOGY
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| ISSN | 0921-8971 |
| 2014-02-01 | |
| 卷号 | 26期号:1页码:91-103 |
| 关键词 | Xanthophylls Biosynthesis Carotenoid Hydroxylase Haematococcus Pluvialis Gene Cloning High Light |
| 产权排序 | [Cui, Hongli; Wang, Yan; Cui, Yulin; Qin, Song] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Biol & Biol Resources Utilizat, Yantai 264003, Peoples R China; [Cui, Hongli] Univ Chinese Acad Sci, Beijing 100049, Peoples R China; [Yu, Xiaona; Liu, Zhaopu] Nanjing Agr Univ, Coll Resources & Environm Sci, Key Lab Marine Biol, Nanjing 210095, Jiangsu, Peoples R China; [Li, Xueqin] Shenzhen Univ, Coll Life Sci, Shenzhen Key Lab Marine Bioresource & Ecoenvironm, Shenzhen 518060, Peoples R China |
| 英文摘要 | A full-length complementary DNA (cDNA) sequence of epsilon-ring CHY (designated Haecyp97c) was cloned from the green alga Haematococcus pluvialis by reverse transcription polymerase chain reaction (RT-PCR) and rapid amplification of cDNA ends methods. The Haecyp97c cDNA sequence was 1,995 base pairs (bp) in length, which contained a 1,620-bp open reading frame, a 46-bp 5'-untranslated region (UTR), and a 329-bp 3'-UTR with the characteristic of the poly (A) tail. The deduced protein had a calculated molecular mass of 58.71 kDa with an estimated isoelectric point of 7.94. Multiple alignment analysis revealed that the deduced amino acid sequence of HaeCYP97C shared high identity of 72-85 % with corresponding CYP97Cs from other eukaryotes. The catalytic motifs of cytochrome P450s were detected in the amino acid sequence of HaeCYP97C. The transcriptional levels of Haecyp97c and xanthophylls accumulation under high light (HL) stress have been examined. The results revealed that Haecyp97c transcript was strongly increased after 13-28 h under HL stress. Meanwhile, the concentrations of chlorophylls, carotenes, and lutein were decreased, and zeaxanthin and astaxanthin concentrations were increased rapidly, respectively. These facts indicated that HaeCYP97C was perhaps involved in xanthophyll biosynthesis, which plays an important role in adaption to HL for H. pluvialis.; A full-length complementary DNA (cDNA) sequence of epsilon-ring CHY (designated Haecyp97c) was cloned from the green alga Haematococcus pluvialis by reverse transcription polymerase chain reaction (RT-PCR) and rapid amplification of cDNA ends methods. The Haecyp97c cDNA sequence was 1,995 base pairs (bp) in length, which contained a 1,620-bp open reading frame, a 46-bp 5'-untranslated region (UTR), and a 329-bp 3'-UTR with the characteristic of the poly (A) tail. The deduced protein had a calculated molecular mass of 58.71 kDa with an estimated isoelectric point of 7.94. Multiple alignment analysis revealed that the deduced amino acid sequence of HaeCYP97C shared high identity of 72-85 % with corresponding CYP97Cs from other eukaryotes. The catalytic motifs of cytochrome P450s were detected in the amino acid sequence of HaeCYP97C. The transcriptional levels of Haecyp97c and xanthophylls accumulation under high light (HL) stress have been examined. The results revealed that Haecyp97c transcript was strongly increased after 13-28 h under HL stress. Meanwhile, the concentrations of chlorophylls, carotenes, and lutein were decreased, and zeaxanthin and astaxanthin concentrations were increased rapidly, respectively. These facts indicated that HaeCYP97C was perhaps involved in xanthophyll biosynthesis, which plays an important role in adaption to HL for H. pluvialis. |
| 文章类型 | Article |
| 资助机构 | National Natural Science Foundation of China [40876082]; International Innovation Partnership Program: Typical Environmental Process and Effects on Resources in Coastal Zone Area; Public Science and Technology Research Funds Projects of the Ocean [200905021, 201205027]; Natural Science Foundation of Shandong Province [ZR2012DQ015]; Guangdong province comprehensive strategic cooperation project of the Chinese Academy of Sciences [2011A090100040]; Science and Technology Planning Project of Yantai, China [2010247] |
| 收录类别 | SCI |
| 语种 | 英语 |
| 关键词[WOS] | CHLORELLA-ZOFINGIENSIS CHLOROPHYTA ; ASTAXANTHIN BIOSYNTHETIC-PATHWAY ; FUNCTIONAL-ANALYSIS ; BETA-RING ; CLUSTAL-W ; ARABIDOPSIS ; ACCUMULATION ; LUTEIN ; PLANTS ; LIGHT |
| 研究领域[WOS] | Biotechnology & Applied Microbiology ; Marine & Freshwater Biology |
| WOS记录号 | WOS:000330969800012 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.yic.ac.cn/handle/133337/7016 |
| 专题 | 海岸带生物学与生物资源利用重点实验室_海岸带生物学与生物资源保护实验室 |
| 通讯作者 | Qin, S (reprint author), Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Biol & Biol Resources Utilizat, Yantai 264003, Peoples R China. [email protected]; [email protected] |
| 作者单位 | 1.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Biol & Biol Resources Utilizat, Yantai 264003, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Nanjing Agr Univ, Coll Resources & Environm Sci, Key Lab Marine Biol, Nanjing 210095, Jiangsu, Peoples R China 4.Shenzhen Univ, Coll Life Sci, Shenzhen Key Lab Marine Bioresource & Ecoenvironm, Shenzhen 518060, Peoples R China |
| 推荐引用方式 GB/T 7714 | Cui, Hongli,Yu, Xiaona,Wang, Yan,et al. Gene cloning and expression profile of a novel carotenoid hydroxylase (CYP97C) from the green alga Haematococcus pluvialis[J]. JOURNAL OF APPLIED PHYCOLOGY,2014,26(1):91-103. |
| APA | Cui, Hongli.,Yu, Xiaona.,Wang, Yan.,Cui, Yulin.,Li, Xueqin.,[email protected].(2014).Gene cloning and expression profile of a novel carotenoid hydroxylase (CYP97C) from the green alga Haematococcus pluvialis.JOURNAL OF APPLIED PHYCOLOGY,26(1),91-103. |
| MLA | Cui, Hongli,et al."Gene cloning and expression profile of a novel carotenoid hydroxylase (CYP97C) from the green alga Haematococcus pluvialis".JOURNAL OF APPLIED PHYCOLOGY 26.1(2014):91-103. |
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