| Induction of oxidative stress and related transcriptional effects of perfluorononanoic acid using an in vivo assessment | |
Yang, Shaoli1 ; Liu, Shengchen2; Ren, Zongming1; Jiao, Xudong1; Qin, Song1; Yang, SL (reprint author), Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China. [email protected]
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| 发表期刊 | COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY
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| ISSN | 1532-0456 |
| 2014-03-01 | |
| 卷号 | 160页码:60-65 |
| 关键词 | Apoptosis Fatty Acid Beta-oxidation Oxidative Stress Perfluorononanoic Acid Zebrafish |
| 产权排序 | [Yang, Shaoli; Ren, Zongming; Jiao, Xudong; Qin, Song] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China; [Liu, Shengchen] Shandong Oriental Ocean Sci Tech Co Ltd, Yantai 264003, Peoples R China |
| 英文摘要 | Perfluorononanoic acid (PFNA) is an organic pollutant ubiquitous in the environment. However, the potential toxicity of PFNA remains largely unknown in teleost fish. This study defined the oxidative stress and related transcriptional effects of PFNA at various concentrations on zebrafish larvae. Activities of superoxide dismutase were induced in PFNA-treated groups but attenuated with exposure to higher concentration. Catalase activity and lipid peroxidation were significantly inhibited or increased at the highest concentration, respectively. To test the apoptotic pathway, several genes related to cell apoptosis were examined using real-time PCR. The expression of p53, apoptosis-inducing factor (AIF) and c-Jun NH (2)-terminal kinase (JNK) was partially increased, while Bcl-2, an anti-apoptotic gene, was reduced, with no significant effects on Bax and caspase-3 during the exposure period. The effect of PFNA on lipid beta-oxidation system was investigated by examining the activity of peroxisome fatty acyl-COA oxidase (ACOX) and the expression of peroxisome proliferating activating receptors (PPARs). ACOX activity was moderately elevated with marginal significance and was not a significant consequence of PPAR alpha and PPAR gamma expression. The overall results suggest that turbulence of oxidative stress and apoptotic pathway is involved in PFNA-induced toxicity in zebrafish larvae, and the gene expression patterns are able to reveal some potential mechanisms of developmental toxicity. Crown Copyright (C) 2013 Published by Elsevier Inc. All rights reserved.; Perfluorononanoic acid (PFNA) is an organic pollutant ubiquitous in the environment. However, the potential toxicity of PFNA remains largely unknown in teleost fish. This study defined the oxidative stress and related transcriptional effects of PFNA at various concentrations on zebrafish larvae. Activities of superoxide dismutase were induced in PFNA-treated groups but attenuated with exposure to higher concentration. Catalase activity and lipid peroxidation were significantly inhibited or increased at the highest concentration, respectively. To test the apoptotic pathway, several genes related to cell apoptosis were examined using real-time PCR. The expression of p53, apoptosis-inducing factor (AIF) and c-Jun NH (2)-terminal kinase (JNK) was partially increased, while Bcl-2, an anti-apoptotic gene, was reduced, with no significant effects on Bax and caspase-3 during the exposure period. The effect of PFNA on lipid beta-oxidation system was investigated by examining the activity of peroxisome fatty acyl-COA oxidase (ACOX) and the expression of peroxisome proliferating activating receptors (PPARs). ACOX activity was moderately elevated with marginal significance and was not a significant consequence of PPAR alpha and PPAR gamma expression. The overall results suggest that turbulence of oxidative stress and apoptotic pathway is involved in PFNA-induced toxicity in zebrafish larvae, and the gene expression patterns are able to reveal some potential mechanisms of developmental toxicity. Crown Copyright (C) 2013 Published by Elsevier Inc. All rights reserved. |
| 文章类型 | Article |
| 资助机构 | National Natural Science Foundation of China [41001336] |
| 收录类别 | SCI |
| 语种 | 英语 |
| 关键词[WOS] | PERFLUOROOCTANE SULFONATE PFOS ; CARBON-CHAIN LENGTH ; ZEBRAFISH EMBRYOS ; GENE-EXPRESSION ; PERFLUORODODECANOIC ACID ; DEVELOPMENTAL TOXICITY ; LIPID-PEROXIDATION ; PROTEOMIC ANALYSIS ; REACTIVE OXYGEN ; APOPTOSIS |
| 研究领域[WOS] | Biochemistry & Molecular Biology ; Endocrinology & Metabolism ; Toxicology ; Zoology |
| WOS记录号 | WOS:000330917400008 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.yic.ac.cn/handle/133337/7012 |
| 专题 | 海岸带生物学与生物资源利用重点实验室_海岸带生物学与生物资源保护实验室 |
| 通讯作者 | Yang, SL (reprint author), Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China. [email protected] |
| 作者单位 | 1.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China 2.Shandong Oriental Ocean Sci Tech Co Ltd, Yantai 264003, Peoples R China |
| 推荐引用方式 GB/T 7714 | Yang, Shaoli,Liu, Shengchen,Ren, Zongming,et al. Induction of oxidative stress and related transcriptional effects of perfluorononanoic acid using an in vivo assessment[J]. COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY,2014,160:60-65. |
| APA | Yang, Shaoli,Liu, Shengchen,Ren, Zongming,Jiao, Xudong,Qin, Song,&Yang, SL .(2014).Induction of oxidative stress and related transcriptional effects of perfluorononanoic acid using an in vivo assessment.COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY,160,60-65. |
| MLA | Yang, Shaoli,et al."Induction of oxidative stress and related transcriptional effects of perfluorononanoic acid using an in vivo assessment".COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY 160(2014):60-65. |
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