A Potentiometric Flow Biosensor Based on Ammonia-Oxidizing Bacteria for the Detection of Toxicity in Water

	A Potentiometric Flow Biosensor Based on Ammonia-Oxidizing Bacteria for the Detection of Toxicity in Water
	Zhang, Qianyu 1,2; Ding, Jiawang 1; Kou, Lijuan 1,2; Qin, Wei1 ; Qin, W (reprint author), Chinese Acad Sci, Yantai Inst Coastal Zone Res YIC,YICCAS, Key Lab Coastal Zone Environm Proc & Ecol Remedia, Shandong Prov Key Lab Coastal Zone Environm Proc, Yantai 264003, Peoples R China. [email protected]; [email protected]; [email protected]; [email protected]
发表期刊	SENSORS
ISSN	1424-8220
	2013-06-01
卷号	13 期号:6 页码:6936-6945
关键词	Potentiometry Flow Biosensors Ion-selective Electrodes Toxicity Nitrosomonas Europaea
产权排序	[Zhang, Qianyu; Ding, Jiawang; Kou, Lijuan; Qin, Wei] Chinese Acad Sci, Yantai Inst Coastal Zone Res YIC,YICCAS, Key Lab Coastal Zone Environm Proc & Ecol Remedia, Shandong Prov Key Lab Coastal Zone Environm Proc, Yantai 264003, Peoples R China; [Zhang, Qianyu; Kou, Lijuan] Univ Chinese Acad Sci, Beijing 100049, Peoples R China
英文摘要	A flow biosensor for the detection of toxicity in water using the ammonia-oxidizing bacterium (AOB) Nitrosomonas europaea as a bioreceptor and a polymeric membrane ammonium-selective electrode as a transducer is described. The system is based on the inhibition effects of toxicants on the activity of AOB, which can be evaluated by measuring the ammonium consumption rates with the ammonium-selective membrane electrode. The AOB cells are immobilized on polyethersulfone membranes packed in a holder, while the membrane electrode is placed downstream in the flow cell. Two specific inhibitors of the ammonia oxidation. allylthiourea and thioacetamide. have been tested. The IC50 values defined as the concentration of an inhibitor causing a 50% reduction in the ammonia oxidation activity have been measured as 0.17 mu M and 0.46 mu M for allylthiourea and thioacetamide, respectively. The proposed sensor offers advantages of simplicity, speed and high sensitivity for measuring toxicity in water.; A flow biosensor for the detection of toxicity in water using the ammonia-oxidizing bacterium (AOB) Nitrosomonas europaea as a bioreceptor and a polymeric membrane ammonium-selective electrode as a transducer is described. The system is based on the inhibition effects of toxicants on the activity of AOB, which can be evaluated by measuring the ammonium consumption rates with the ammonium-selective membrane electrode. The AOB cells are immobilized on polyethersulfone membranes packed in a holder, while the membrane electrode is placed downstream in the flow cell. Two specific inhibitors of the ammonia oxidation. allylthiourea and thioacetamide. have been tested. The IC50 values defined as the concentration of an inhibitor causing a 50% reduction in the ammonia oxidation activity have been measured as 0.17 mu M and 0.46 mu M for allylthiourea and thioacetamide, respectively. The proposed sensor offers advantages of simplicity, speed and high sensitivity for measuring toxicity in water.
文章类型	Article
资助机构	Instrument Developing Project of the Chinese Academy of Sciences [YZ201161]; National Natural Science Foundation of China [41176081, 21207156]; Taishan Scholar Program of Shandong Province [TS20081159]
收录类别	SCI
语种	英语
关键词[WOS]	NITROSOMONAS-EUROPAEA ; NITRIFICATION ; INHIBITORS ; BIOASSAY
研究领域[WOS]	Chemistry ; Electrochemistry ; Instruments & Instrumentation
WOS记录号	WOS:000320767600004
引用统计	被引频次：19[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.yic.ac.cn/handle/133337/6986
专题	中国科学院海岸带环境过程与生态修复重点实验室
通讯作者	Qin, W (reprint author), Chinese Acad Sci, Yantai Inst Coastal Zone Res YIC,YICCAS, Key Lab Coastal Zone Environm Proc & Ecol Remedia, Shandong Prov Key Lab Coastal Zone Environm Proc, Yantai 264003, Peoples R China. [email protected]; [email protected]; [email protected]; [email protected]
作者单位	1.Chinese Acad Sci, Yantai Inst Coastal Zone Res YIC,YICCAS, Key Lab Coastal Zone Environm Proc & Ecol Remedia, Shandong Prov Key Lab Coastal Zone Environm Proc, Yantai 264003, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Zhang, Qianyu,Ding, Jiawang,Kou, Lijuan,et al. A Potentiometric Flow Biosensor Based on Ammonia-Oxidizing Bacteria for the Detection of Toxicity in Water[J]. SENSORS,2013,13(6):6936-6945.
APA	Zhang, Qianyu.,Ding, Jiawang.,Kou, Lijuan.,Qin, Wei.,Qin, W .,[email protected].(2013).A Potentiometric Flow Biosensor Based on Ammonia-Oxidizing Bacteria for the Detection of Toxicity in Water.SENSORS,13(6),6936-6945.
MLA	Zhang, Qianyu,et al."A Potentiometric Flow Biosensor Based on Ammonia-Oxidizing Bacteria for the Detection of Toxicity in Water".SENSORS 13.6(2013):6936-6945.

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