Chemodosimeter-based fluorescent detection of L-cysteine after extracted by molecularly imprinted polymers

	Chemodosimeter-based fluorescent detection of L-cysteine after extracted by molecularly imprinted polymers
	Cai, Xiaoqiang 1,2,3; Li, Jinhua2,3 ; Zhang, Zhong 2,3,5; Wang, Gang2,3 ; Song, Xingliang 4; You, Jinmao 1; Chen, Lingxin1,2,3
发表期刊	TALANTA
ISSN	0039-9140
	2014-03-01
卷号	120 页码:297-303
关键词	Molecularly Imprinted Polymer Fluorescence Detection L-cysteine Chemodosimeter Rhodamine b
产权排序	[Cai, Xiaoqiang; You, Jinmao; Chen, Lingxin] Qufu Normal Univ, Coll Chem & Chem Engn, Qufu 273165, Peoples R China; [Cai, Xiaoqiang; Li, Jinhua; Zhang, Zhong; Wang, Gang; Chen, Lingxin] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Environm Proc & Ecol Remediat, Yantai 264003, Peoples R China; [Cai, Xiaoqiang; Li, Jinhua; Zhang, Zhong; Wang, Gang; Chen, Lingxin] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Shandong Prov Key Lab Coastal Environm Proc, Yantai 264003, Peoples R China; [Song, Xingliang] Linyi Univ, Sch Chem & Chem Engn, Linyi 276005, Peoples R China; [Zhang, Zhong] Univ Chinese Acad Sci, Beijing 100049, Peoples R China
通讯作者	Chen, LX (reprint author), Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China. [email protected]
英文摘要	A chemodosimeter-based fluorescent detection method coupled with molecularly imprinted polymers (MIPs) extraction was developed for determination of L-cysteine (L-Cys) by combining molecular imprinting technique with fluorescent chemodosimeter. The MIPs prepared by precipitation polymerization with L-Cys as template, possessed high specific surface area of 145 m(2)/g and good thermal stability without decomposition lower than 300 degrees C, and were successfully applied as an adsorbent with excellent selectivity for L-Cys over other amino acids, and enantioselectivity was also demonstrated. A novel chemodosimeter, rhodamine B1, was synthesized for discriminating L-Cys from its structurally similar homocysteine and glutathione as well as various possibly co-existing biospecies in aqueous solutions with notable fluorescence enhancement when adding L-Cys. As L-Cys was added with increasing concentrations, an emission band peaked at 580 nm occurred and significantly increased in fluorescence intensity, by which the L-Cys could be sensed optically. High detectability up to 12.5 nM was obtained. An excellent linearity was found within the wide range of 0.05-50 mu M (r=0.9996), and reasonable relative standard deviations ranging from 0.3% to 3.5% were attained. Such typical features as high selectivity, high sensitivity, easy operation and low cost enabled this MIPs-fluorometry to be potentially applicable for routine detection of trace L-Cys. (C) 2013 Elsevier B.V. All rights reserved.; A chemodosimeter-based fluorescent detection method coupled with molecularly imprinted polymers (MIPs) extraction was developed for determination of L-cysteine (L-Cys) by combining molecular imprinting technique with fluorescent chemodosimeter. The MIPs prepared by precipitation polymerization with L-Cys as template, possessed high specific surface area of 145 m(2)/g and good thermal stability without decomposition lower than 300 degrees C, and were successfully applied as an adsorbent with excellent selectivity for L-Cys over other amino acids, and enantioselectivity was also demonstrated. A novel chemodosimeter, rhodamine B1, was synthesized for discriminating L-Cys from its structurally similar homocysteine and glutathione as well as various possibly co-existing biospecies in aqueous solutions with notable fluorescence enhancement when adding L-Cys. As L-Cys was added with increasing concentrations, an emission band peaked at 580 nm occurred and significantly increased in fluorescence intensity, by which the L-Cys could be sensed optically. High detectability up to 12.5 nM was obtained. An excellent linearity was found within the wide range of 0.05-50 mu M (r=0.9996), and reasonable relative standard deviations ranging from 0.3% to 3.5% were attained. Such typical features as high selectivity, high sensitivity, easy operation and low cost enabled this MIPs-fluorometry to be potentially applicable for routine detection of trace L-Cys. (C) 2013 Elsevier B.V. All rights reserved.
文章类型	Article
资助机构	National Natural Science Foundation of China [21105117, 21275158, 21275068, 21275089]; Natural Science Foundation of Shandong Province of China [ZR2010BQ027]; Chinese Academy of Sciences
收录类别	SCI
语种	英语
关键词[WOS]	COLORIMETRIC SENSOR ; GOLD NANOPARTICLES ; AMINO-ACIDS ; RECOGNITION ; ELECTRODE ; DERIVATIZATION ; CHROMATOGRAPHY ; HOMOCYSTEINE ; PLASMA
研究领域[WOS]	Chemistry
WOS记录号	WOS:000331917700040
引用统计	被引频次：25[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.yic.ac.cn/handle/133337/6900
专题	中国科学院海岸带环境过程与生态修复重点实验室海岸带生物学与生物资源利用重点实验室_海岸带生物学与生物资源保护实验室
作者单位	1.Qufu Normal Univ, Coll Chem & Chem Engn, Qufu 273165, Peoples R China 2.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Environm Proc & Ecol Remediat, Yantai 264003, Peoples R China 3.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Shandong Prov Key Lab Coastal Environm Proc, Yantai 264003, Peoples R China 4.Linyi Univ, Sch Chem & Chem Engn, Linyi 276005, Peoples R China 5.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Cai, Xiaoqiang,Li, Jinhua,Zhang, Zhong,et al. Chemodosimeter-based fluorescent detection of L-cysteine after extracted by molecularly imprinted polymers[J]. TALANTA,2014,120:297-303.
APA	Cai, Xiaoqiang.,Li, Jinhua.,Zhang, Zhong.,Wang, Gang.,Song, Xingliang.,...&Chen, Lingxin.(2014).Chemodosimeter-based fluorescent detection of L-cysteine after extracted by molecularly imprinted polymers.TALANTA,120,297-303.
MLA	Cai, Xiaoqiang,et al."Chemodosimeter-based fluorescent detection of L-cysteine after extracted by molecularly imprinted polymers".TALANTA 120(2014):297-303.

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