| Regulation of Metabolites, Gene Expression, and Antioxidant Enzymes to Environmentally Relevant Lead and Zinc in the Halophyte Suaeda salsa | |
Wu, Huifeng1 ; Liu, Xiaoli1,2; Zhao, Jianmin1; Yu, Junbao1
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| 发表期刊 | JOURNAL OF PLANT GROWTH REGULATION
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| ISSN | 0721-7595 |
| 2013-06-01 | |
| 卷号 | 32期号:2页码:353-361 |
| 关键词 | Lead Zinc Biomarker Suaeda Salsa Metabolomics Antioxidant Enzyme |
| 产权排序 | [Wu, Huifeng; Liu, Xiaoli; Zhao, Jianmin; Yu, Junbao] Chinese Acad Sci, Key Lab Coastal Zone Environm Proc, Shandong Prov Key Lab Coastal Zone Environm Proc, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China; [Liu, Xiaoli] Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China |
| 通讯作者 | Wu, HF (reprint author), Chinese Acad Sci, Key Lab Coastal Zone Environm Proc, Shandong Prov Key Lab Coastal Zone Environm Proc, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China. [email protected] |
| 作者部门 | 中科院海岸带环境过程与生态修复重点实验室 |
| 英文摘要 | As a pioneer halophyte, Suaeda salsa can grow in the intertidal zones, which are often polluted by heavy metals from both terrigenous wastewater and tidewater containing high concentrations of heavy metals. Therefore S. salsa is potentially suitable as a biomonitor for heavy-metal pollution in the intertidal zones. In this study, regulation of metabolites, gene expression, and antioxidant status of environmentally relevant lead and zinc were characterized using NMR-based metabolomics, real-time quantitative reverse transcription polymerase chain reaction, and antioxidant enzyme activities. In Pb-exposed S. salsa samples, only decreased tyrosine was observed, with statistical significance approaching 0.05. Metabolic biomarkers in Zn-exposed S. salsa samples included increased amino acids (valine, isoleucine, leucine, threonine, asparagine, and phenylalanine), and decreased acetate, glucose, ferulate, and fumarate. Increased succinate, aspartate, and malonate and decreased fructose were uniquely found in mixed Pb- and Zn-exposed samples in addition to the similar metabolic changes such as alanine, glucose, fumarate, and ferulate in Zn-exposed samples. Based on the metabolic biomarkers, gene expressions, and antioxidant enzyme activities, both Zn and mixed Pb and Zn induced significant oxidative stress and disturbances in energy metabolism, photosynthesis/glucogenesis, and protein biodegradation in S. salsa. However, environmentally relevant Pb could induce slight oxidative stress in S. salsa as indicated by increased catalase gene expression levels and catalase activities.; As a pioneer halophyte, Suaeda salsa can grow in the intertidal zones, which are often polluted by heavy metals from both terrigenous wastewater and tidewater containing high concentrations of heavy metals. Therefore S. salsa is potentially suitable as a biomonitor for heavy-metal pollution in the intertidal zones. In this study, regulation of metabolites, gene expression, and antioxidant status of environmentally relevant lead and zinc were characterized using NMR-based metabolomics, real-time quantitative reverse transcription polymerase chain reaction, and antioxidant enzyme activities. In Pb-exposed S. salsa samples, only decreased tyrosine was observed, with statistical significance approaching 0.05. Metabolic biomarkers in Zn-exposed S. salsa samples included increased amino acids (valine, isoleucine, leucine, threonine, asparagine, and phenylalanine), and decreased acetate, glucose, ferulate, and fumarate. Increased succinate, aspartate, and malonate and decreased fructose were uniquely found in mixed Pb- and Zn-exposed samples in addition to the similar metabolic changes such as alanine, glucose, fumarate, and ferulate in Zn-exposed samples. Based on the metabolic biomarkers, gene expressions, and antioxidant enzyme activities, both Zn and mixed Pb and Zn induced significant oxidative stress and disturbances in energy metabolism, photosynthesis/glucogenesis, and protein biodegradation in S. salsa. However, environmentally relevant Pb could induce slight oxidative stress in S. salsa as indicated by increased catalase gene expression levels and catalase activities. |
| 文章类型 | Article |
| 资助机构 | Project of National Science and Technology Pillar Program [2011BAC02B01]; Chinese Academy of Sciences |
| 收录类别 | SCI |
| 语种 | 英语 |
| 关键词[WOS] | V-H+-ATPASE ; OSMOTIC-STRESS ; METABOLOMICS ; LEAVES ; NACL ; TOLERANCE ; EXPOSURE ; PPASE |
| 研究领域[WOS] | Plant Sciences |
| WOS记录号 | WOS:000319165500012 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.yic.ac.cn/handle/133337/6660 |
| 专题 | 海岸带生物学与生物资源利用重点实验室_海岸带生物学与生物资源保护实验室 中国科学院海岸带环境过程与生态修复重点实验室_滨海湿地实验室 中国科学院海岸带环境过程与生态修复重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Key Lab Coastal Zone Environm Proc, Shandong Prov Key Lab Coastal Zone Environm Proc, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wu, Huifeng,Liu, Xiaoli,Zhao, Jianmin,et al. Regulation of Metabolites, Gene Expression, and Antioxidant Enzymes to Environmentally Relevant Lead and Zinc in the Halophyte Suaeda salsa[J]. JOURNAL OF PLANT GROWTH REGULATION,2013,32(2):353-361. |
| APA | Wu, Huifeng,Liu, Xiaoli,Zhao, Jianmin,&Yu, Junbao.(2013).Regulation of Metabolites, Gene Expression, and Antioxidant Enzymes to Environmentally Relevant Lead and Zinc in the Halophyte Suaeda salsa.JOURNAL OF PLANT GROWTH REGULATION,32(2),353-361. |
| MLA | Wu, Huifeng,et al."Regulation of Metabolites, Gene Expression, and Antioxidant Enzymes to Environmentally Relevant Lead and Zinc in the Halophyte Suaeda salsa".JOURNAL OF PLANT GROWTH REGULATION 32.2(2013):353-361. |
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