| Undecylprodigiosin Induced Apoptosis in P388 Cancer Cells Is Associated with Its Binding to Ribosome | |
| Liu, Ping1,2; Wang, Yuan-yuan1; Qi, Xin1; Gu, QianQun1; Geng, Meiyu3; Li, Jing1 | |
| 发表期刊 | PLOS ONE
 |
| ISSN | 1932-6203 |
| 2013-06-14 | |
| 卷号 | 8期号:6页码:e65381 |
| 关键词 | Cycloprodigiosin Hydrochloride h+/cl-symporter Hl-60 Cells h+-atpase In-vitro Prodigiosin Lines Differentiation Proliferation Inhibition |
| 产权排序 | [Liu, Ping; Wang, Yuan-yuan; Qi, Xin; Gu, QianQun; Li, Jing] Ocean Univ China, Sch Med & Pharm, Minist Educ, Key Lab Marine Drugs, Qingdao, Peoples R China; [Liu, Ping] Chinese Acad Sci, Anal & Testing Ctr, Yantai Inst Coastal Zone Res, Yantai, Peoples R China; [Geng, Meiyu] Chinese Acad Sci, Shanghai Inst Mat Med, Key Lab Drug Res, Shanghai 200031, Peoples R China |
| 通讯作者 | Geng, MY (reprint author), Chinese Acad Sci, Shanghai Inst Mat Med, Key Lab Drug Res, Shanghai 200031, Peoples R China. [email protected] ; [email protected] |
| 作者部门 | 海岸带生物学与生物资源利用所重点实验室 |
| 英文摘要 | Prodigiosins (PGs) are a family of natural red pigments with anticancer activity, and one member of the family has entered clinical phase II trials. However, the anticancer mechanisms of PGs remain largely unclear. This study was designed to investigate the molecular basis of anticancer activity of UP, a derivative of PGs, in P388 cells. By introducing pharmacological inhibitors and utilizing a variety of analytical approaches including western blotting, flow cytometry and confocal laser microscopy, we found that UP inhibited proliferation of P388 via arresting cells at G2/M phase and inducing cells apoptosis, which was related to the activation of P38, JNK rather than ERK1/2 signaling. ROS regeneration and acidification in cells appear not involved in UP induced apoptosis. Furthermore, utilizing mass spectrometry, sucrose density gradient fractionation and immunofluorescence staining, we discovered that UP was apparently located at ribosome. These results together indicate that ribosome may be the potential target of UP in cancer cells, which opened a new avenue in delineating the anticancer mechanism of PGs.; Prodigiosins (PGs) are a family of natural red pigments with anticancer activity, and one member of the family has entered clinical phase II trials. However, the anticancer mechanisms of PGs remain largely unclear. This study was designed to investigate the molecular basis of anticancer activity of UP, a derivative of PGs, in P388 cells. By introducing pharmacological inhibitors and utilizing a variety of analytical approaches including western blotting, flow cytometry and confocal laser microscopy, we found that UP inhibited proliferation of P388 via arresting cells at G2/M phase and inducing cells apoptosis, which was related to the activation of P38, JNK rather than ERK1/2 signaling. ROS regeneration and acidification in cells appear not involved in UP induced apoptosis. Furthermore, utilizing mass spectrometry, sucrose density gradient fractionation and immunofluorescence staining, we discovered that UP was apparently located at ribosome. These results together indicate that ribosome may be the potential target of UP in cancer cells, which opened a new avenue in delineating the anticancer mechanism of PGs. |
| 文章类型 | Article |
| 资助机构 | National High-tech R&D Program of China [2011AA09070104]; Program for Changjiang Scholars and Innovative Research Team in University [IRT0944] |
| 收录类别 | SCI |
| 语种 | 英语 |
| 关键词[WOS] | CYCLOPRODIGIOSIN HYDROCHLORIDE ; H+/CL-SYMPORTER ; HL-60 CELLS ; H+-ATPASE ; IN-VITRO ; PRODIGIOSIN ; LINES ; DIFFERENTIATION ; PROLIFERATION ; INHIBITION |
| 研究领域[WOS] | Science & Technology - Other Topics |
| WOS记录号 | WOS:000320363300018 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.yic.ac.cn/handle/133337/6474 |
| 专题 | 海岸带生物学与生物资源利用重点实验室_海岸带生物学与生物资源保护实验室 |
| 作者单位 | 1.Ocean Univ China, Sch Med & Pharm, Minist Educ, Key Lab Marine Drugs, Qingdao, Peoples R China 2.Chinese Acad Sci, Anal & Testing Ctr, Yantai Inst Coastal Zone Res, Yantai, Peoples R China 3.Chinese Acad Sci, Shanghai Inst Mat Med, Key Lab Drug Res, Shanghai 200031, Peoples R China |
| 推荐引用方式 GB/T 7714 | Liu, Ping,Wang, Yuan-yuan,Qi, Xin,et al. Undecylprodigiosin Induced Apoptosis in P388 Cancer Cells Is Associated with Its Binding to Ribosome[J]. PLOS ONE,2013,8(6):e65381. |
| APA | Liu, Ping,Wang, Yuan-yuan,Qi, Xin,Gu, QianQun,Geng, Meiyu,&Li, Jing.(2013).Undecylprodigiosin Induced Apoptosis in P388 Cancer Cells Is Associated with Its Binding to Ribosome.PLOS ONE,8(6),e65381. |
| MLA | Liu, Ping,et al."Undecylprodigiosin Induced Apoptosis in P388 Cancer Cells Is Associated with Its Binding to Ribosome".PLOS ONE 8.6(2013):e65381. |
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