| Effects of long-term chlorimuron-ethyl application on the diversity and antifungal activity of soil Pseudomonas spp. in a soybean field in Northeast China | |
Wang, Jingjing1,3; Zhang, Huiwen1; Zhang, Xiaoli2 ; Qin, Shenghong1,3; Tan, Huanbo1,3; Li, Xinyu1
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| 发表期刊 | ANNALS OF MICROBIOLOGY
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| ISSN | 1590-4261 |
| 2013-03-01 | |
| 卷号 | 63期号:1页码:335-341 |
| 关键词 | Soil Pseudomonas Antifungal Activity Antifungal Metabolites Chlorimuron-ethyl Chronic Effect Ardra Analysis |
| 产权排序 | [Wang, Jingjing; Zhang, Huiwen; Qin, Shenghong; Tan, Huanbo; Li, Xinyu] Chinese Acad Sci, Inst Appl Ecol, State Key Lab Forest & Soil Ecol, Shenyang 110016, Peoples R China; [Zhang, Xiaoli] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China; [Wang, Jingjing; Qin, Shenghong; Tan, Huanbo] Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China |
| 通讯作者 | Li, XY (reprint author), Chinese Acad Sci, Inst Appl Ecol, State Key Lab Forest & Soil Ecol, Shenyang 110016, Peoples R China. [email protected] |
| 作者部门 | 海岸带生物学与生物资源利用所重点实验室 |
| 英文摘要 | The herbicide chlorimuron-ethyl has been applied widely for weed control in farmland, especially in soybean fields in China over the past decade, but the chronic effects of this herbicide on soil microorganisms, particularly Pseudomonas spp., is not well understood. Taking a continuously cropped soybean field in the town of Fuyuan-a soybean production base of Heilongjiang Province in Northeast China-as a case study, soil samples were collected from plots having received 0-, 5-, and 10-year applications of chlorimuron-ethyl (30 g active component of chlorimuron-ethyl/ha/year) to study the abundance and diversity of Pseudomonas spp. Meanwhile, an in vitro assay was used to examine the antifungal activities of isolated Pseudomonas spp. against soil-borne pathogens (Fusarium graminearum, Fusarium oxysporum, and Rhizoctonia solani) causing soybean root rot disease. The production of siderophore, hydrogen cyanide (HCN), and lytic enzymes (cellulase, pectinase, and chitinase) by Pseudomonas spp. was also investigated. With 5- and 10- year chlorimuron-ethyl application, the numbers of soil Pseudomonas spp. decreased from 121 x 10(2) CFU/g dry soil in the control to 40 x 10(2) CFU/g dry soil and 13 x 10(2) CFU/g dry soil, and the Shannon index values decreased from 6.23 to 3.71 and 1.73, respectively. The numbers of antifungal Pseudomonas spp. also decreased, and the proportions of Pseudomonas spp. with antifungal activities against the different test pathogens altered. All the antifungal Pseudomonas spp. could produce siderophore and HCN but not lytic enzymes. The results suggest that long-term application of chlorimuron-ethyl in continuously cropped soybean field had negative effects on the abundance and diversity of soil Pseudomonas spp., including species with different antifungal activities against pathogens. Siderophore and HCN rather than lytic enzymes formed the antifungal metabolites of Pseudomonas spp., and the number of antifungal Pseudomonas that can produce siderophore and HCN decreased markedly under application of chlorimuron-ethyl, especially after 10-year application.; The herbicide chlorimuron-ethyl has been applied widely for weed control in farmland, especially in soybean fields in China over the past decade, but the chronic effects of this herbicide on soil microorganisms, particularly Pseudomonas spp., is not well understood. Taking a continuously cropped soybean field in the town of Fuyuan-a soybean production base of Heilongjiang Province in Northeast China-as a case study, soil samples were collected from plots having received 0-, 5-, and 10-year applications of chlorimuron-ethyl (30 g active component of chlorimuron-ethyl/ha/year) to study the abundance and diversity of Pseudomonas spp. Meanwhile, an in vitro assay was used to examine the antifungal activities of isolated Pseudomonas spp. against soil-borne pathogens (Fusarium graminearum, Fusarium oxysporum, and Rhizoctonia solani) causing soybean root rot disease. The production of siderophore, hydrogen cyanide (HCN), and lytic enzymes (cellulase, pectinase, and chitinase) by Pseudomonas spp. was also investigated. With 5- and 10- year chlorimuron-ethyl application, the numbers of soil Pseudomonas spp. decreased from 121 x 10(2) CFU/g dry soil in the control to 40 x 10(2) CFU/g dry soil and 13 x 10(2) CFU/g dry soil, and the Shannon index values decreased from 6.23 to 3.71 and 1.73, respectively. The numbers of antifungal Pseudomonas spp. also decreased, and the proportions of Pseudomonas spp. with antifungal activities against the different test pathogens altered. All the antifungal Pseudomonas spp. could produce siderophore and HCN but not lytic enzymes. The results suggest that long-term application of chlorimuron-ethyl in continuously cropped soybean field had negative effects on the abundance and diversity of soil Pseudomonas spp., including species with different antifungal activities against pathogens. Siderophore and HCN rather than lytic enzymes formed the antifungal metabolites of Pseudomonas spp., and the number of antifungal Pseudomonas that can produce siderophore and HCN decreased markedly under application of chlorimuron-ethyl, especially after 10-year application. |
| 文章类型 | Article |
| 资助机构 | National High Technology Research and Development Program (863 Program) [2012AA101403]; National Science Foundation [41071202] |
| 收录类别 | SCI |
| 语种 | 英语 |
| 关键词[WOS] | FLUORESCENT PSEUDOMONADS ; ANTIBIOTIC PRODUCTION ; MICROBIAL DIVERSITY ; RHIZOSPHERE ; PLANT ; PCR |
| 研究领域[WOS] | Biotechnology & Applied Microbiology ; Microbiology |
| WOS记录号 | WOS:000314692400036 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.yic.ac.cn/handle/133337/6432 |
| 专题 | 海岸带生物学与生物资源利用重点实验室_海岸带生物学与生物资源保护实验室 |
| 作者单位 | 1.Chinese Acad Sci, Inst Appl Ecol, State Key Lab Forest & Soil Ecol, Shenyang 110016, Peoples R China 2.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China 3.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Jingjing,Zhang, Huiwen,Zhang, Xiaoli,et al. Effects of long-term chlorimuron-ethyl application on the diversity and antifungal activity of soil Pseudomonas spp. in a soybean field in Northeast China[J]. ANNALS OF MICROBIOLOGY,2013,63(1):335-341. |
| APA | Wang, Jingjing,Zhang, Huiwen,Zhang, Xiaoli,Qin, Shenghong,Tan, Huanbo,&Li, Xinyu.(2013).Effects of long-term chlorimuron-ethyl application on the diversity and antifungal activity of soil Pseudomonas spp. in a soybean field in Northeast China.ANNALS OF MICROBIOLOGY,63(1),335-341. |
| MLA | Wang, Jingjing,et al."Effects of long-term chlorimuron-ethyl application on the diversity and antifungal activity of soil Pseudomonas spp. in a soybean field in Northeast China".ANNALS OF MICROBIOLOGY 63.1(2013):335-341. |
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