Bacterial and archaeal assemblages in sediments of a large shallow freshwater lake, Lake Taihu, as revealed by denaturing gradient gel electrophoresis

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	Bacterial and archaeal assemblages in sediments of a large shallow freshwater lake, Lake Taihu, as revealed by denaturing gradient gel electrophoresis
	Liu, F. H. ; Lin, G. H. ; Gao, G. ; Qin, B. Q. ; Zhang, J. S. ; Zhao, G. P. ; Zhou, Z. H. ; Shen, J. H.
部门归属	海岸带生物学与生物资源利用所重点实验室
通讯作者	Shen, JH (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Plant Physiol & Ecol, Key Lab Synthet Biol, 300 Fenglin Rd, Shanghai 200032, Peoples R China.,[email protected]
学科主题	Biotechnology & Applied Microbiology ; Microbiology
关键词	16s Ribosomal-rna Bacterioplankton Community Composition Cyanobacterial Blooms Recent Eutrophication Microbial Community Diversity China Pcr Dynamics Plankton
出处	JOURNAL OF APPLIED MICROBIOLOGY
ISSN号	1364-5072
	2009
卷	106 期:3 页:1022-1032
收录类别	SCI
产权排序	[Liu, F. H.; Lin, G. H.; Zhang, J. S.; Zhao, G. P.; Zhou, Z. H.; Shen, J. H.] Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Plant Physiol & Ecol, Key Lab Synthet Biol, Shanghai 200032, Peoples R China; [Liu, F. H.] Chinese Acad Sci, Grad Sch, Beijing, Peoples R China; [Gao, G.; Qin, B. Q.] Chinese Acad Sci, Nanjing Inst Geog & Limnol, Nanjing, Peoples R China
资助信息	Taihu Laboratory for Lake Ecosystem Research (TLLER); Chinese Academy of Sciences
英文摘要	To explore the association of microbial community structure with the development of eutrophication in a large shallow freshwater lake, Lake Taihu. The bacterial and archaeal assemblages in sediments of different lake areas were analysed using denaturing gradient gel electrophoresis (DGGE) of amplified 16S rDNA fragments. The bacterial DGGE profiles showed that eutrophied sites, grass-bottom areas and relatively clean sites with a eutrophic (albeit dredged) site are three respective clusters. Fifty-one dominant bacterial DGGE bands were detected and 92 corresponding clones were sequenced, most of which were affiliated with bacterial phylotypes commonly found in freshwater ecosystems. Actinobacteria were detected in the centre of the lake and not at eutrophied sites whereas the opposite was found with respect to Verrucomicrobiales. Twenty-five dominant archaeal DGGE bands were detected and 31 corresponding clones were sequenced, most of which were affiliated with freshwater archaeal phylotypes. The bacterial community structures in the sediments of different areas with similar water quality and situation tend to be similar in Taihu Lake. This study may expand our knowledge on the relationship between the overall microbial assemblages and the development of eutrophication in the shallow freshwater lake.
语种	英语
文献类型	共享期刊论文
条目标识符	http://ir.yic.ac.cn/handle/133337/6322
专题	科研共享资源
推荐引用方式 GB/T 7714	Liu, F. H.,Lin, G. H.,Gao, G.,et al. Bacterial and archaeal assemblages in sediments of a large shallow freshwater lake, Lake Taihu, as revealed by denaturing gradient gel electrophoresis. 2009.

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