In Vitro Antitumor Activities of Low Molecular Sea Cucumber Stichopus japonicus Peptides Sequentially Hydrolyzed by Proteases

	In Vitro Antitumor Activities of Low Molecular Sea Cucumber Stichopus japonicus Peptides Sequentially Hydrolyzed by Proteases
	Zhou, Xiaoqiu ; Wang, Changhai ; Jiang, Aili
通讯作者	Zhou, XQ (reprint author), Chinese Acad Sci, Yantai Inst Coastal Zone Res Sustainable Dev, Yantai 264003, Peoples R China.,[email protected] ; [email protected] ; [email protected]
	2012
会议名称	International Conference on Biotechnology, Chemical and Materials Engineering (CBCME 2011)
页码	1259_1262
会议日期	DEC 28-29, 2011
会议地点	Kunming, PEOPLES R CHINA
ISSN号	1022-6680
ISBN号	978-3-03785-307-8
产权排序	[Zhou, Xiaoqiu] Chinese Acad Sci, Yantai Inst Coastal Zone Res Sustainable Dev, Yantai 264003, Peoples R China
关键词	Stichopus Japonicus Peptides Ultrafiltration Antitumor Activities
摘要	The sea cucumber (Stichopus japonicus) was continuously hydrolyzed by pepsin, trypsin to get sea cucumber peptides (SCP), and low molecular peptides (LSCP), MW < 3000 Da, were collected through an ultrafiltration membrane with a molecular weight cut-off of 3000 Da Two main fractions, LSCP-1 and LSCP-2 were obtained by one step gel filtration column chromatography. Furthermore, the antitumor activities, in vitro, of LSCP-1 and LSCP-2 fractions were evaluated by MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide) assay. LSCP-2 showed significantly antitumor activity against the SGC-7901 and MCF-7 cell lines, LSCP-1 showed significantly antitumor activity only against SGC-7901 cell line, and two fractions did not show activity against A549 cell line. Overall, this study indicated that LSCP posseses potential to treat gastric cancer and breast cancer.
学科领域	Multidisciplinary ; Biotechnology & Applied Microbiology ; Chemical ; Engineering ; Materials Science
URL	查看原文
收录类别	CPCI（ISTP）
语种	英语
文献类型	会议论文
条目标识符	http://ir.yic.ac.cn/handle/133337/6257
专题	中国科学院烟台海岸带研究所知识产出
推荐引用方式 GB/T 7714	Zhou, Xiaoqiu,Wang, Changhai,Jiang, Aili. In Vitro Antitumor Activities of Low Molecular Sea Cucumber Stichopus japonicus Peptides Sequentially Hydrolyzed by Proteases[C]:TRANS TECH PUBLICATIONS LTD,2012:1259_1262.