| Accurate quantification of astaxanthin from Haematococcus crude extract spectrophotometrically | |
Li Yeguang1; Miao Fengping1,2 ; Geng Yahong1; Lu Dayan1; Zhang Chengwu3; Zeng Mingtao4
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| 发表期刊 | CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY
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| ISSN | 0254-4059 |
| 2012-07-01 | |
| 卷号 | 30期号:4页码:627-637 |
| 关键词 | Haematococcus Pluvialis Astaxanthin Quantification Spectrophotometry |
| 产权排序 | [Li Yeguang; Miao Fengping; Geng Yahong; Lu Dayan] Chinese Acad Sci, Wuhan Bot Garden, Key Lab Plant Germplasm Enhancement & Special Agr, Wuhan 430074, Peoples R China; [Miao Fengping] Chinese Acad Sci, Yantai Inst Costal Zone Res, Lab Coastal Zone Bioresources, Yantai 264003, Peoples R China; [Zhang Chengwu] Jinan Univ, Inst Hydrobiol, Guangzhou 510632, Guangdong, Peoples R China; [Zeng Mingtao] Texas Tech Univ, Dept Biomed Sci, Hlth Sci Ctr, Paul L Foster Sch Med, El Paso, TX 79905 USA |
| 通讯作者 | Li, YG (reprint author), Chinese Acad Sci, Wuhan Bot Garden, Key Lab Plant Germplasm Enhancement & Special Agr, Wuhan 430074, Peoples R China.,[email protected] |
| 作者部门 | 海岸带生物学与生物资源利用所重点实验室 |
| 英文摘要 | The influence of alkali on astaxanthin and the optimal working wave length for measurement of astaxanthin from Haematococcus crude extract were investigated, and a spectrophotometric method for precise quantification of the astaxanthin based on the method of Boussiba et al. was established. According to Boussiba's method, alkali treatment destroys chlorophyll. However, we found that: 1) carotenoid content declined for about 25% in Haematococcus fresh cysts and up to 30% in dry powder of Haematococcus broken cysts after alkali treatment; and 2) dimethyl sulfoxide (DMSO)-extracted chlorophyll of green Haematococcus bares little absorption at 520-550 nm. Interestingly, a good linear relationship existed between absorbance at 530 nm and astaxanthin content, while an unknown interference at 540-550 nm was detected in our study. Therefore, with 530 nm as working wavelength, the alkali treatment to destroy chlorophyll was not necessary and the influence of chlorophyll, other carotenoids, and the unknown interference could be avoided. The astaxanthin contents of two samples were measured at 492 nm and 530 nm; the measured values at 530 nm were 2.617 g/100 g and 1.811 g/100 g. When compared with the measured values at 492 nm, the measured values at 530 nm decreased by 6.93% and 11.96%, respectively. The measured values at 530 nm are closer to the true astaxanthin contents in the samples. The data show that 530 nm is the most suitable wave length for spectrophotometric determination to the astaxanthin in Haematococcus crude extract.; The influence of alkali on astaxanthin and the optimal working wave length for measurement of astaxanthin from Haematococcus crude extract were investigated, and a spectrophotometric method for precise quantification of the astaxanthin based on the method of Boussiba et al. was established. According to Boussiba's method, alkali treatment destroys chlorophyll. However, we found that: 1) carotenoid content declined for about 25% in Haematococcus fresh cysts and up to 30% in dry powder of Haematococcus broken cysts after alkali treatment; and 2) dimethyl sulfoxide (DMSO)-extracted chlorophyll of green Haematococcus bares little absorption at 520-550 nm. Interestingly, a good linear relationship existed between absorbance at 530 nm and astaxanthin content, while an unknown interference at 540-550 nm was detected in our study. Therefore, with 530 nm as working wavelength, the alkali treatment to destroy chlorophyll was not necessary and the influence of chlorophyll, other carotenoids, and the unknown interference could be avoided. The astaxanthin contents of two samples were measured at 492 nm and 530 nm; the measured values at 530 nm were 2.617 g/100 g and 1.811 g/100 g. When compared with the measured values at 492 nm, the measured values at 530 nm decreased by 6.93% and 11.96%, respectively. The measured values at 530 nm are closer to the true astaxanthin contents in the samples. The data show that 530 nm is the most suitable wave length for spectrophotometric determination to the astaxanthin in Haematococcus crude extract. |
| 文章类型 | Article |
| 资助机构 | Yunnan Provincial Sciences and Technology Department, China[2007AD009]; National Natural Science Foundation of China[CNSF30570183]; Knowledge Innovation Program of Chinese Academy of Sciences[KSCX2-YW-G-060] |
| 收录类别 | SCI |
| 语种 | 英语 |
| 关键词[WOS] | FATTY-ACIDS ; PLUVIALIS ; ACCUMULATION ; GREEN ; ESTERS ; HYDROXYLASE ; NITROGEN ; STAGE |
| 研究领域[WOS] | Marine & Freshwater Biology ; Oceanography |
| WOS记录号 | WOS:000305906700015 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.yic.ac.cn/handle/133337/6090 |
| 专题 | 海岸带生物学与生物资源利用重点实验室_海岸带生物学与生物资源保护实验室 |
| 作者单位 | 1.Chinese Acad Sci, Wuhan Bot Garden, Key Lab Plant Germplasm Enhancement & Special Agr, Wuhan 430074, Peoples R China 2.Chinese Acad Sci, Yantai Inst Costal Zone Res, Lab Coastal Zone Bioresources, Yantai 264003, Peoples R China 3.Jinan Univ, Inst Hydrobiol, Guangzhou 510632, Guangdong, Peoples R China 4.Texas Tech Univ, Dept Biomed Sci, Hlth Sci Ctr, Paul L Foster Sch Med, El Paso, TX 79905 USA |
| 推荐引用方式 GB/T 7714 | Li Yeguang,Miao Fengping,Geng Yahong,et al. Accurate quantification of astaxanthin from Haematococcus crude extract spectrophotometrically[J]. CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY,2012,30(4):627-637. |
| APA | Li Yeguang,Miao Fengping,Geng Yahong,Lu Dayan,Zhang Chengwu,&Zeng Mingtao.(2012).Accurate quantification of astaxanthin from Haematococcus crude extract spectrophotometrically.CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY,30(4),627-637. |
| MLA | Li Yeguang,et al."Accurate quantification of astaxanthin from Haematococcus crude extract spectrophotometrically".CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY 30.4(2012):627-637. |
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